Assessment of siRNA pharmacokinetics using ELISA-based quantification

Journal of Controlled Release

Volumn 143, Issue 1, 2010, Pages 80-87

  Kim, Eun Joong ^a   Park, Tae Gwan ^b   Oh, Yu Kyoung ^a   Shim, Chang Koo ^a  

^a Seoul National University   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Chemical modification; ELISA; Lipoplexes; Pharmacokinetics; Quantification; SiRNA

Indexed keywords

BACKGROUND LEVEL; BLOOD CONCENTRATIONS; CATIONIC LIPOSOMES; CHEMICALLY MODIFIED; CLEARANCE RATES; COEFFICIENT OF VARIATION; DELIVERY SYSTEMS; DINITROPHENOLS; ELISA; ELISA-BASED ASSAYS; HELA CELL; IN-VIVO; LIPOPLEXES; PHARMACOKINETIC PROFILES; QUANTIFICATION METHODS;

BLOOD; CHEMICAL MODIFICATION; LIPOSOMES; NUCLEIC ACIDS; PHARMACOKINETICS; PHENOLS; PHOSPHOLIPIDS;

RNA;

1,2 DIOLEOYL 3 TRIMETHYLAMMONIOPROPANE; 2,4 DINITROPHENOL; BIOTIN; DOUBLE STRANDED RNA; SINGLE STRANDED RNA; SMALL INTERFERING RNA;

ACCURACY; ANIMAL EXPERIMENT; ARTICLE; CELL STRAIN; CONTROLLED STUDY; DRUG BLOOD LEVEL; DRUG CLEARANCE; DRUG DELIVERY SYSTEM; DRUG STABILITY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPERIMENTAL MOUSE; FEMALE; FLUOROMETRY; GENETIC TRANSFECTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE STUDY; RNA METHYLATION; SENSITIVITY AND SPECIFICITY; SIGNAL DETECTION;

ANIMALS; BIOTINYLATION; CATIONS; DINITROPHENOLS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FATTY ACIDS, MONOUNSATURATED; FEMALE; FLUOROMETRY; HELA CELLS; HUMANS; INJECTIONS, INTRAVENOUS; LIPOSOMES; METHYLATION; MICE; MICE, INBRED BALB C; NUCLEIC ACID CONFORMATION; OBSERVER VARIATION; QUATERNARY AMMONIUM COMPOUNDS; REPRODUCIBILITY OF RESULTS; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 77950519511     PISSN: 01683659     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jconrel.2009.12.004     Document Type: Article

References (23)

1. Carthew R.W., Sontheimer E.J. Origins and mechanisms of miRNAs and siRNAs. Cell 2009, 136:642-655.
2. Bumcrot D., Manoharan M., Koteliansky V., Sah D.W. RNAi therapeutics: a potential new class of pharmaceutical drugs. Nat. Chem. Biol. 2006, 2:711-719.
3. Melnikova I. RNA-based therapies. Nat. Rev. Drug Dis. 2007, 6:863-864.
4. Haussecker D. The business of RNAi therapeutics. Hum. Gene Ther. 2008, 19:451-462.
5. Watts J.K., Deleavey G.F., Damha M.J. Chemically modified siRNA: tools and applications. Drug Discov. Today 2008, 13:842-855.
6. Judge A.D., Bola G., Lee A.C., MacLachlan I. Design of noninflammatory synthetic siRNA mediating potent gene silencing in vivo. Mol. Ther. 2006, 13:494-505.
7. Cekaite L., Furset G., Hovig E., Sioud M. Gene expression analysis in blood cells in response to unmodified and 2'-modified siRNAs reveals TLR-dependent and independent effects. J. Mol. Biol. 2007, 365:90-108.
8. Zhang S., Zhao B., Jiang H., Wang B., Ma B. Cationic lipids and polymers mediated vectors for delivery of siRNA. J. Control. Release 2007, 123:1-10.
9. de Fougerolles A.R. Delivery vehicles for small interfering RNA in vivo. Hum. Gene Ther. 2008, 19:125-132.
10. Whitehead K.A., Langer R., Anderson D.G. Knocking down barriers: advances in siRNA delivery. Nat. Rev. Drug Discov. 2009, 8:129-138.
11. Raymond C.K., Roberts B.S., Garrett-Engele P., Lim L.P., Johnson J.M. Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAs. RNA 2005, 11:1737-1744.
12. Chen C., Ridzon D.A., Broomer A.J., Zhou Z., Lee D.H., Nguyen J.T., Barbisin M., Xu N.L., Mahuvakar V.R., Andersen M.R., Lao K.Q., Livak K.J., Guegler K.J. Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res. 2005, 33:e179.
13. Liu W.L., Stevenson M., Seymour L.W., Fisher K.D. Quantification of siRNA using competitive qPCR. Nucleic Acids Res. 2009, 37:e4.
14. Jeong G.J., Byun H.M., Kim J.M., Yoon H., Choi H.G., Kim W.K., Kim S.J., Oh Y.K. Biodistribution and tissue expression kinetics of plasmid DNA complexed with polyethylenimines of different molecular weight and structure. J. Control. Release 2007, 118:118-125.
15. Merkel O.M., Librizzi D., Pfestroff A., Schurrat T., Buyens K., Sanders N.N., De Smedt S.C., Béhé M., Kissel T. Stability of siRNA polyplexes from poly(ethylenimine) and poly(ethylenimine)-g-poly(ethylene glycol) under in vivo conditions: effects on pharmacokinetics and biodistribution measured by Fluorescence Fluctuation Spectroscopy and Single Photon Emission Computed Tomography (SPECT) imaging. J. Control. Release 2009, 138:148-159.
16. Veldhoen S., Laufer S.D., Trampe A., Restle T. Cellular delivery of small interfering RNA by a non-covalently attached cell-penetrating peptide: quantitative analysis of uptake and biological effect. Nucleic Acids Res. 2006, 34:6561-6573.
17. Merkel O.M., Librizzi D., Pfestroff A., Schurrat T., Béhé M., Kissel T. In vivo SPECT and real-time gamma camera imaging of biodistribution and pharmacokinetics of siRNA delivery using an optimized radiolabeling and purification procedure. Bioconjug. Chem. 2009, 20:174-182.
18. Chen A.A., Derfus A.M., Khetani S.R., Bhatia S.N. Quantum dots to monitor RNAi delivery and improve gene silencing. Nucleic Acids Res. 2005, 33:e190.
19. Mikhaylova M., Stasinopoulos I., Kato Y., Artemov D., Bhujwalla Z.M. Imaging of cationic multifunctional liposome-mediated delivery of COX-2 siRNA. Cancer Gene Ther. 2009, 16:217-226.
20. Kim S.H., Kim E.J., Hou J.H., Kim J.M., Choi H.G., Shim C.K., Oh Y.K. Opsonized erythrocyte ghosts for liver-targeted delivery of antisense oligodeoxynucleotides. Biomaterials 2009, 30:959-967.
21. Ocker M., Neureiter D., Lueders M., Zopf S., Ganslmayer M., Hahn E.G., Herold C., Schuppan D. Variants of bcl-2 specific siRNA for silencing antiapoptotic bcl-2 in pancreatic cancer. Gut 2005, 54:1298-1308.
22. Choung S., Kim Y.J., Kim S., Park H.O., Choi Y.C. Chemical modification of siRNAs to improve serum stability without loss of efficacy. Biochem. Biophys. Res. Commun. 2006, 342:919-927.
23. de Wolf H.K., Snel C.J., Verbaan F.J., Schiffelers R.M., Hennink W.E., Storm G. Effect of cationic carriers on the pharmacokinetics and tumor localization of nucleic acids after intravenous administration. Int. J. Pharm. 2007, 331:167-175.