Loop-mediated isothermal amplification method for rapid detection of Vibrio alginolyticus, the causative agent of vibriosis in mariculture fish

Letters in Applied Microbiology

Volumn 50, Issue 5, 2010, Pages 480-485

  Cai, S H ^a,b   Lu, Y S ^a,b   Wu, Z H ^a,b   Jian, J C ^a,b   Wang, B ^a,b   Huang, Y C ^a,b  

^a GUANGDONG OCEAN UNIVERSITY   (China)

^b Guangdong Provincial Key Lab of Pathogenic Biology Epidemiology for Aquatic Economic Animals   (China)

Author keywords

Loop mediated isothermal amplification; PCR; Rapid detection; Vibrio alginolyticus

Indexed keywords

DNA; GENES; MARINE BIOLOGY; MEATS;

AGAR GEL; BACTERIAL STRAINS; CAUSATIVE AGENTS; CROSS REACTION; DETECTION SENSITIVITY; DIAGNOSTIC TOOLS; DNA POLYMERASE; FISH TISSUE; HIGH SPECIFICITY; LAMP-PRODUCTS; NAKED-EYE; RAPID DETECTION; SYBR GREEN; VIBRIO ALGINOLYTICUS; WATER BATHS;

AMPLIFICATION;

DNA POLYMERASE; BACTERIAL PROTEIN; PRIMER DNA;

BACTERIUM; DETECTION METHOD; DNA; FISH; MARICULTURE; POLYMERASE CHAIN REACTION;

ANIMAL TISSUE; ARTICLE; BACTERIUM DETECTION; CONTROLLED STUDY; DNA EXTRACTION; FISH DISEASE; GENE TARGETING; HEART; INCUBATION TEMPERATURE; KIDNEY PARENCHYMA; LIVER; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; MARICULTURE; MUSCLE TISSUE; NONHUMAN; NUCLEOTIDE SEQUENCE; REACTION TIME; SENSITIVITY AND SPECIFICITY; TEMPERATURE; VIBRIO ALGINOLYTICUS; VIBRIOSIS; ANIMAL; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; NUCLEIC ACID AMPLIFICATION; PROCEDURES; VETERINARY;

BACTERIA (MICROORGANISMS); VIBRIO ALGINOLYTICUS;

ANIMALS; BACTERIAL PROTEINS; DNA PRIMERS; FISH DISEASES; NUCLEIC ACID AMPLIFICATION TECHNIQUES; VIBRIO ALGINOLYTICUS; VIBRIO INFECTIONS;

EID: 77950481239     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2010.02823.x     Document Type: Article

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