Integration of exogenous DNA into mouse embryonic stem cell chromosomes shows preference into genes and frequent modification at junctions

Chromosome Research

Volumn 18, Issue 2, 2010, Pages 191-201

  Suzuki, Keiichiro ^a   Ohbayashi, Fumi ^a   Nikaido, Itoshi ^a   Okuda, Akihiko ^a   Takaki, Haruyoshi ^b   Okazaki, Yasushi ^a   Mitani, Kohnosuke ^a  

^a SAITAMA MEDICAL UNIVERSITY   (Japan)

^b TEIKYO UNIVERSITY   (Japan)

Author keywords

Chromosomal integration ; Exogenous dna ; Integration sites ; Mouse embryonic stem cells

Indexed keywords

GENOMIC DNA;

ANIMAL CELL; ARTICLE; CHROMOSOME DELETION; CHROMOSOME INSERTION; CODON; COMPUTER SIMULATION; CONTROLLED STUDY; CPG ISLAND; DNA DETERMINATION; DNA INTEGRATION; DNA MODIFICATION; ELECTROPORATION; EMBRYONIC STEM CELL; GAP JUNCTION; GENE EXPRESSION; MICROARRAY ANALYSIS; MOUSE; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYADENYLATION; PRIORITY JOURNAL; PROTEIN MODIFICATION; TRANSCRIPTION INITIATION SITE;

ANIMALS; CHROMOSOMES, MAMMALIAN; ELECTROPORATION; EMBRYONIC STEM CELLS; MICE; MUTAGENESIS, INSERTIONAL; PLASMIDS; REPETITIVE SEQUENCES, NUCLEIC ACID; TRANSCRIPTION, GENETIC;

MAMMALIA;

EID: 77950460776     PISSN: 09673849     EISSN: 15736849     Source Type: Journal    
DOI: 10.1007/s10577-010-9111-5     Document Type: Article

References (34)

1. Bodley AL, Huang HC, Yu C, Liu LF (1993) Integration of simian, virus 40 into cellular DNA occurs at or near topoisomerase II cleavage hot spots induced by VM-26 (teniposide). MoI Cell Biol 13:6190-6200
2. Bushman F, Lewinski M, Ciuffi A et al (2005) Genome-wide analysis of retroviral DNA integration. Nat Rev Microbiol 3:848-858 (Pubitemid 41693180)
3. Chen ZY, Yant SR, He CY, Meuse L, Shen S, Kay MA (2001) Linear DNAs concatemerize in vivo and. result in sustained transgene expression in mouse liver. Mol Ther 3:403-410 (Pubitemid 32514657)
4. Dellaire G, Chartrand P (1998) Direct evidence that transgene integration is random in murine cells, implying that naturally occurring double-strand breaks may be distributed similarly within the genome. Radiat Res 149:325-329 (Pubitemid 28150924)
5. Dellaire G, Yan J, Little KC, Drouin R, Chartrand P (2002) Evidence that extrachromosomal double-strand break repair can be coupled to the repair of chromosomal double-strand breaks in mammalian cells. Chromosoma 111:304-312 (Pubitemid 36067403)
6. Derse D, Crise B, Li Y et al (2007) Human T-cell leukemia virus type 1 integration target sites in the human genome: comparison with those of other retroviruses. J Virol 81:6731-6741 (Pubitemid 46878077)
7. Fujimaki K, Aratani Y, Fujisawa S, Motomura S, Okubo T, Koyama H (1996) DNA topoisomerase II inhibitors enhance random integration of transfected vectors into human chromosomes. Somat Cell Mol Genet 22:279-290 (Pubitemid 27043733)
8. Garrick D, Fiering S, Martin DI, Whitelaw E (1998) Repeatinduced gene silencing in mammals. Nat Genet 18:56-59
9. Hacein-Bey-Abina S, Von Kalle C, Schmidt. M et al (2003) LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-Xl. Science 302: 415-419
10. Hu WS, Pathak VK (2000) Design of retroviral vectors and helper cells for gene therapy. Pharmacol Rev 52:493-511
11. Inagaki K, Lewis SM, Wu X et al (2007) DNA palindromes with a modest arm length, of greater, similar 20 base pairs are a significant target for recombinant adeno-associated virus vector integration in the liver, muscles, and heart in mice. J Virol 81:11290-11303
12. Kabotyanski EB, Gomelsky L, Han JO, Stamato TD, Roth DB (1998) Double-strand break repair in Ku86- and XRCC4deficient cells. Nucleic Acids Res 26:5333-5342
13. Kay MA, Glorioso JC, Naldini L (2001) Viral vectors for gene therapy: the art of turning infectious agents into vehicles of therapeutics. Nat Med 7:33-40
14. Lin Y, Waldman AS (2001) Capture of DNA sequences at double-strand breaks in mammalian chromosomes. Genetics 158:1665-1674 (Pubitemid 32783147)
15. Merrihew RV, Marburger K, Pennington SL, Roth. DB, Wilson JH (1996) High-frequency illegitimate integration of transfected DNA at preintegrated target sites in a mammalian genome. Mol Cell Biol 16:10-18 (Pubitemid 126506945)
16. Miller DG, Petek LM, Russell DW (2004) Adeno-associated virus vectors integrate at chromosome breakage sites. Nat Genet 36:767-773
17. Miller DG, Trobridge GD, Petek LM, Jacobs MA, Kaul R, Russell DW (2005) Large-scale analysis of adenoassociated virus vector integration sites in normal human cells. J Virol 79:11434-11442 (Pubitemid 41170684)
18. Mitchell RS, Beitzel BF, Schroder AR et al (2004) Retroviral DNA integration: ASLV, HIV, and MLV show distinct target site preferences. PLoS Biol 2:E234
19. Nakai H, Montmi E, Fuess S et al (2003) Helper-independent and AAV-ITR.-independent chromosomal integration of double-stranded linear DNA vectors in mice. Mol Ther 7:101-111 (Pubitemid 36312924)
20. Nakai H, Wu X, Fuess S et al (2005) Large-scale molecular characterization of adeno-associated virus vector integration in mouse liver. J Virol 79:3606-3614 (Pubitemid 40327949)
21. Ohbayashi F, Balamotis MA, Kishimoto A et al (2005) Correction of chromosomal mutation and random integration in embryonic stem cells with helper-dependent adenoviral vectors. Proc Natl Acad Sci USA 102:1362813633 (Pubitemid 41420899)
22. Ramirez-Solis R, Davis AC, Bradley A. (1993) Gene targeting in embryonic stem cells. Methods Enzymol 225:855-878
23. Rassool FV, McKeithan TW, Neilly ME, van Melle E, Espinosa R 3 rd, Le Beau MM (1991) Preferential integration of marker DNA into the chromosomal fragile site at 3pl4: an approach to cloning fragile sites. Proc Natl Acad. Sci USA 88:6657-6661
24. Rijkers T, Peetz A, Ruther U (1994) Insertional mutagenesis in transgenic mice. Transgenic Res 3:203-215
25. Roth DB, Porter TN, Wilson JH (1985) Mechanisms of nonhomologous recombination in mammalian cells. Mol Cell Biol 5:2599-2607
26. Secretan MB, Scuric Z, Oshima J et al (2004) Effect of Ku86 and DNA-PKcs deficiency on non-homologous endjoining and homologous recombination using a transient transfection assay. Mutat Res 554:351-364 (Pubitemid 39140841)
27. Smith. K (2001) Theoretical mechanisms in targeted and random integration of transgene DNA. Reprod Nutr Dev 41:465-485
28. Soriano P, Montgomery C, Geske R, Bradley A. (1991) Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice. Cell 64:693-702
29. Takata. M, Sasaki MS, Sonoda E et al (1998) Homologous recombination and non-homologous end-joining pathways of DNA double-strand break, repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells. Embo J 17:5497-5508 (Pubitemid 28427063)
30. Thrasher A, Gaspar B (2007) Severe Adverse Event in Clinical Trial of Gene Therapy for X-SCID. http://www.asgt.org/UserFiles/XSCID statement.pdf
31. Toneguzzo F, Keating A, Glynn S, McDonald K (1988) Electric field-mediated gene transfer: characterization of DNA transfer and patterns of integration in lymphoid cells. Nucleic Acids Res 16:5515-5532
32. Wang H, Perrault AR, Takeda Y, Qin W, Iliakis G (2003) Biochemical evidence for Ku-ind.epend.ent http://Ku-ind.epend.ent backup pathways ofNHEJ. Nucleic Acids Res 31:5377-5388
33. Wang H, Shayakhmetov DM, Leege T et al (2005) A capsidmodified helper-dependent adenovirus vector containing the beta-globin locus control region displays a nonrandom integration pattern and allows stable, erythroid-specific gene expression. J Virol 79:10999-11013 (Pubitemid 41170641)
34. Wurtele H, Little KC, Chartrand. P (2003) Illegitimate DNA integration in mammalian cells. Gene Ther 10:1791-1799 (Pubitemid 37185136)