The ribosome-bound initiation factor 2 recruits initiator tRNA to the 30S initiation complex

EMBO Reports

Volumn 11, Issue 4, 2010, Pages 312-316

  Milon, Pohl ^a   Carotti, Marcello ^b   Konevega, Andrey L ^a,c   Wintermeyer, Wolfgang ^a   Rodnina, Marina V ^a   Gualerzi, Claudio O ^b  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b UNIVERSITY OF CAMERINO   (Italy)

^c PETERSBURG NUCLEAR PHYSICS INSTITUTE   (Russian Federation)

Author keywords

FRET; GTPase; Rapid filtration; Stopped flow fluorescence

Indexed keywords

INITIATION FACTOR 2; TRANSFER RNA;

ARTICLE; BINDING AFFINITY; BINDING KINETICS; CELL ANCHORAGE; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; GENE LOCATION; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN RNA BINDING; RIBOSOME SUBUNIT;

FLUORESCENCE RESONANCE ENERGY TRANSFER; KINETICS; MODELS, BIOLOGICAL; PROKARYOTIC INITIATION FACTOR-2; RIBOSOME SUBUNITS, SMALL, BACTERIAL; RIBOSOMES; RNA, TRANSFER, MET;

BACTERIA (MICROORGANISMS); EUKARYOTA;

EID: 77950371305     PISSN: 1469221X     EISSN: 14693178     Source Type: Journal    
DOI: 10.1038/embor.2010.12     Document Type: Article

References (21)

1. Acker MG, Shin BS, Nanda JS, Saini AK, Dever TE, Lorsch JR (2009) Kinetic analysis of late steps of eukaryotic translation initiation. J Mol Biol 385: 491-506
2. Antoun A, Pavlov MY, Lovmar M, Ehrenberg M (2006a) How initiation factors maximize the accuracy of tRNA selection in initiation of bacterial protein synthesis. Mol Cell 23: 183-193
3. Antoun A, Pavlov MY, Lovmar M, Ehrenberg M (2006b) How initiation factors tune the rate of initiation of protein synthesis in bacteria. EMBO J 25: 2539-2550
4. Brandi L, Fabbretti A, Milon P, Carotti M, Pon CL, Gualerzi CO (2007) Methods for identifying compounds that specifically target translation. Methods Enzymol 431: 229-267
5. Canonaco MA, Calogero RA, Gualerzi CO (1986) Mechanism of translational initiation in prokaryotes. Evidence for a direct effect of IF2 on the activity of the 30S ribosomal subunit. FEBS Lett 207: 198-204
6. Caserta E, Tomsic J, Spurio R, La Teana A, Pon CL, Gualerzi CO (2006) Translation initiation factor IF2 interacts with the 30S ribosomal subunit via two separate binding sites. J Mol Biol 362: 787-799
7. Grunberg-Manago M, Dessen P, Pantaloni D, Godefroy-Colburn T, Wolfe AD, Dondon J (1975) Light-scattering studies showing the effect of initiation factors on the reversible dissociation of Escherichia coli ribosomes. J Mol Biol 94: 461-478
8. Gualerzi CO, Wintermeyer W (1986) Prokaryotic initiation factor 2 acts at the level of the 30S ribosomal subunit. A fluorescence stopped-flow study. FEBS Lett 202: 1-6
9. Gualerzi CO, Brandi L, Caserta E, Garofalo C, Lammi M, La Teana A, Petrelli D, Spurio R, Tomsic J, Pon CL (2001) Initiation factors in the early events of mRNA translation in bacteria. Cold Spring Harb Symp Quant Biol 66: 363-376
10. Guillon L, Schmitt E, Blanquet S, Mechulam Y (2005) Initiator tRNA binding by e/aIF5B, the eukaryotic/archaeal homologue of bacterial initiation factor IF2. Biochemistry 44: 15594-15601
11. Hershey JW, Merrick WC (2000) Pathway and mechanism of initiation of protein synthesis. In Translational Control of Gene Expression, Sonenberg N, Hershey JW, Mathew MB (eds) pp33-88. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory
12. Kapp LD, Lorsch JR (2004) GTP-dependent recognition of the methionine moiety on initiator tRNA by translation factor eIF2. J Mol Biol 335: 923-936
13. Laursen BS, Sorensen HP, Mortensen KK, Sperling-Petersen HU (2005) Initiation of protein synthesis in bacteria. Microbiol Mol Biol Rev 69: 101-123
14. Milon P, Konevega AL, Peske F, Fabbretti A, Gualerzi CO, Rodnina MV (2007) Transient kinetics, fluorescence, and FRET in studies of initiation of translation in bacteria. Methods Enzymol 430: 1-30
15. Milon P, Konevega AL, Gualerzi CO, Rodnina MV (2008) Kinetic checkpoint at a late step in translation initiation. Mol Cell 30: 712-720
16. Paleskava A, Konevega AL, Rodnina MV (2009) Thermodynamic and kinetic framework of selenocysteyl-tRNASec recognition by elongation factor SelB. J Biol Chem 285: 3014-3020
17. Rodnina MV, Wintermeyer W (1995) GTP consumption of elongation factor Tu during translation of heteropolymeric mRNAs. Proc Natl Acad Sci USA 92: 1945-1949
18. Shin BS, Maag D, Roll-Mecak A, Arefin MS, Burley SK, Lorsch JR, Dever TE (2002) Uncoupling of initiation factor eIF5B/IF2 GTPase and translational activities by mutations that lower ribosome affinity. Cell 111: 1015-1025
19. Simonetti A, Marzi S, Myasnikov AG, Fabbretti A, Yusupov M, Gualerzi CO, Klaholz BP (2008) Structure of the 30S translation initiation complex. Nature 455: 416-420
20. Phe binding to 30S ribosomal subunits. A fluorescence stopped-flow study. Biochemistry 22: 690-694
21. Wu XQ, RajBhandary UL (1997) Effect of the amino acid attached to Escherichia coli initiator tRNA on its affinity for the initiation factor IF2 and on the IF2 dependence of its binding to the ribosome. J Biol Chem 272: 1891-1895