High-yield expression and purification of the transmembrane region of ion channel-forming amyloid-β protein for NMR structural studies

Process Biochemistry

Volumn 45, Issue 5, 2010, Pages 682-688

  Park, Tae Joon ^a   Im, Sooyeon ^a   Kim, Ji Sun ^a   Kim, Yongae ^a  

^a Hankuk University of Foreign Studies   (South Korea)

Author keywords

Alzheimer's disease; Amyloid channel; Integral membrane protein; Multimeric coding sequence; NMR; Transmembrane protein

Indexed keywords

ALZHEIMER'S DISEASE; CODING SEQUENCES; INTEGRAL MEMBRANE PROTEINS; MULTIMERIC; TRANS-MEMBRANE PROTEINS;

ACTIVATION ANALYSIS; AFFINITY CHROMATOGRAPHY; BIOLOGICAL MEMBRANES; CALCIUM; CELL MEMBRANES; CYTOLOGY; GENE EXPRESSION; GLYCOPROTEINS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MEMBRANES; NUCLEAR MAGNETIC RESONANCE; PUMPING PLANTS; PURIFICATION;

PROTEINS;

EUKARYOTA;

EID: 77950300278     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2010.01.002     Document Type: Article

References (33)

1. Bowie J.U. Membrane proteins. Are we destined to repeat history? Editorial overview. Curr Opin Struct Biol 10 (2000) 435-437
2. Russell R.B., and Eggleston D.S. New roles for structure in biology and drug discovery. Nat Struct Biol 7 Suppl. (2000) 928-930
3. Wilken J., and Kent S.B. Chemical protein synthesis. Curr Opin Biotechnol 9 (1998) 412-426
4. Dawson P.E., and Kent S.B. Synthesis of native proteins by chemical ligation. Annu Rev Biochem 69 (2000) 923-960
5. Kochendoerfer G.G. Chemical protein synthesis methods in drug discovery. Curr Opin Drug Discov Devel 4 (2001) 205-214
6. Braun P., and LaBaer J. High throughput protein production for functional proteomics. Trends Biotechnol 21 (2003) 383-388
7. Farid S.S. Process economics of industrial monoclonal antibody manufacture. J Chromatogr 848 (2007) 8-18
8. Peti W., and Page R. Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost. Protein Expr Purif 51 (2007) 1-10
9. Glenner G.G., and Wong C.W. Alzheimer's disease and down's syndrome sharing of a unique cerebrovascular amyloid fibril protein. Biochem Biophys Res Commun 122 (1984) 1131-1135
10. Masters C.L., Simms G., Weinmann N.A., Multhaup G., McDonald B.L., and Beyreuther K. Amyloid plaque core protein in Alzheimer disease and down syndrome. Proc Natl Acad Sci USA 82 (1985) 4245-4249
11. Hartley D.M., Walsh D.M., Ye C.P., Diehl T., Vasquez S., Vassilev P.M., et al. Protofibrillar intermediates of amyloid β-protein induce acute electrophysiological changes and progressive neurotoxicity in cortical neurons. J Neurosci 19 (1999) 8876-8884
12. 1-42 are potent central nervous system neurotoxins. Proc Natl Acad Sci USA 95 (1998) 6448-6453
13. Walsh D.M., Klyubin I., Fadeeva J.V., Cullen W.K., Anwyl R., Wolfe M.S., et al. Naturally secreted oligomers of amyloid beta protein potently inhibit hippocampal long-term potentiation in vivo. Nature 416 (2002) 535-539
14. McLean C.A., Cherny R.A., Fraser F.W., Fuller S.J., Smith M.J., Beyreuther K., et al. Soluble pool of A beta amyloid as a determinant of severity of neurodegeneration in Alzheimer's disease. Ann Neurol 46 (1990) 860-866
15. Lin H., Bhatia R., and Lal R. Amyloid β protein forms ion channels: implications for Alzheimer's disease pathophysiology. FASEB J 15 (2001) 2433-2444
16. Yun Z., Charles M., Thomas D., Brian N., Barrett C.F., Alexander B., et al. Identification of genes expressed in C. elegans touch receptor neurons. Nature 418 (2002) 331-335
17. Khachaturian Z.S. The role of calcium regulation in brain aging: reexamination of a hypothesis. Aging (Milano) 1 (1989) 17-34
18. Mattson M.P., Tomaselli K.J., and Rydel R.E. Calcium-destabilizing and neurodegenerative effects of aggregated 13-amyloid peptide are attenuated by basic FGF. Brain Res 621 (1993) 35-49
19. 2+ homeostasis in Alzheimer's disease. Trends Neurosci 16 (1993) 409-414
20. Mattson M.P., Cheng B., Davis D., Bryant K., Lieberburg I., and Rydel R. 13-Amyloid peptides destabilize calcium homeostasis and render human cortical neurons vulnerable to excitotoxicity. J Neurosci 12 (1992) 376-386
21. Sisodia S.S. 13-Amyloid precursor protein cleavage by a membrane-bound protease. Proc Natl Acad Sci USA 89 (1992) 6075-6079
22. De Strooper B., and Annaert W. Proteolytic processing and cell biological functions of the amyloid precursor protein. J Cell Sci 113 (2000) 1857-1870
23. Selkoe D.J. Alzheimer's disease: genes, proteins, and therapy. Physiol Rev 81 (2001) 741-766
24. Delaglio F., Grzesiek S., Vuister G.W., Zhu G., Pfeifer J., and Bax A. NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6 (1995) 277-293
25. Jang H., Zheng J., and Nussinov R. Models of beta-amyloid ion channels in the membrane suggest that channel formation in the bilayer is a dynamic process. Biophys J 93 (2007) 1938-1949
26. Jang H., Zheng J., Lal R., and Nussinov R. New structures help the modeling of toxic amyloidbeta ion channels. Trends Biochem Sci 33 (2008) 91-100
27. Jang H., Ma B., Lal R., and Nussinov R. Models of toxic beta-sheet channels of protegrin-1 suggest a common subunit organization motif shared with toxic Alzheimer beta-amyloid ion channels. Biophys J 95 (2008) 4631-4642
28. Jiao Y., and Yang P. Molecular modeling of the ion channel-like nanotube structure of amyloid β-peptide. Chinese Sci Bull 52 (2007) 1576-1580
29. Khang T., Choi J., Franzin C.M., and Marassi F.M. Bcl-XL as a fusion protein for the high-level expression of membrane-associated proteins. Protein Sci 14 (2005) 948-955
30. Park T.J., Kim J.S., Choi S.S., and Kim Y. Cloning, expression, isotope labeling, purification, and characterization of bovine antimicrobial peptide, lactophoricin in Escherichia coli. Protein Expr Purif 65 (2009) 23-29
31. Park T.J., Choi S.S., Gang G.A., and Kim Y. High-level expression and purification of the second transmembrane domain of wild-type and mutant human melanocortin-4 receptor for solid-state NMR structural studies. Protein Expr Purif 62 (2008) 139-145
32. Opella S.J., and Marassi F.M. Structure determination of membrane proteins by NMR spectroscopy. Chem Rev 104 (2004) 3587-3606
33. Franzin C.M., Gong X.M., Thai K., Yu J., and Marassi F.M. NMR of membrane proteins in micelles and bilayers: the FXYD family proteins. Methods 41 (2007) 398-408