The use of biochemical and biophysical tools for triage of high-throughput screening hits - aa case study with escherichia coli phosphopantetheine adenylyltransferase

Chemical Biology and Drug Design

Volumn 75, Issue 5, 2010, Pages 444-454

  Miller, J Richard ^a,b   Thanabal, Venkataraman ^a   Melnick, Michael M ^a,c   Lall, Manjinder ^a   Donovan, Charles ^a   Sarver, Ronald W ^a,d   Lee, Doh Yeel ^a   Ohren, Jeff ^a   Emerson, Don ^a,e  

^a PFIZER INC   (United States)

^b MERCK RESEARCH LABORATORIES   (United States)

^c Kalexsyn Inc   (United States)

^d Neogen Corporation   (United States)

^e Resonance Technologies   (United States)

Author keywords

19F NMR; Antibacterials; Biophysics; Calorimetry; Phosphopantetheine; Screening; Triage

Indexed keywords

9 OXO 4,9 DIHYDROPYRAZOLO[5,1 B]QUINAZOLINE 2 CARBOXYLIC ACID DERIVATIVE; ADENOSINE TRIPHOSPHATE; ANTIINFECTIVE AGENT; CARBOXYLIC ACID DERIVATIVE; PANTETHEINE PHOSPHATE; PANTETHEINE PHOSPHATE ADENYLYLTRANSFERASE; TRANSFERASE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BACTERIAL STRAIN; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; DRUG STRUCTURE; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME KINETICS; ESCHERICHIA COLI; FLUORINE NUCLEAR MAGNETIC RESONANCE; HIGH THROUGHPUT SCREENING; IC 50; ISOTHERMAL TITRATION CALORIMETRY; MOLECULAR INTERACTION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; ORTHOGONAL ASSAY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SATURATION TRANSFER DIFFERENCE NUCLEAR MAGNETIC RESONANCE; STRUCTURE ACTIVITY RELATION; THERMODYNAMICS;

ANTI-BACTERIAL AGENTS; BINDING, COMPETITIVE; BIOPHYSICAL PHENOMENA; CALORIMETRY; CATALYTIC DOMAIN; ESCHERICHIA COLI; FLUORINE; HIGH-THROUGHPUT SCREENING ASSAYS; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; NUCLEOTIDYLTRANSFERASES; QUINAZOLINES; STRUCTURE-ACTIVITY RELATIONSHIP;

ESCHERICHIA COLI;

EID: 77949996564     PISSN: 17470277     EISSN: 17470285     Source Type: Journal    
DOI: 10.1111/j.1747-0285.2010.00957.x     Document Type: Article

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