A search for Trypanosoma brucei rhodesiense diagnostic antigens by proteomic screening and targeted cloning

PLoS ONE

Volumn 5, Issue 3, 2010, Pages

  Manful, Theresa ^a   Mulindwa, Julius ^a,b   Frank, Fernanda M ^c   Clayton, Christine E ^a   Matovu, Enock ^b  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b MAKERERE UNIVERSITY   (Uganda)

^c UNIVERSIDAD DE BUENOS AIRES   (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN; HEAT SHOCK PROTEIN 70; HISTONE H2B; HISTONE H3; NUCLEOSIDE TRANSPORTER; PARAFLAGELLAR ROD PROTEIN 1; PHOSPHOGLYCERATE KINASE; UNCLASSIFIED DRUG; HISTONE;

AFRICAN TRYPANOSOMIASIS; ARTICLE; CLINICAL ARTICLE; CLONING; CONTROLLED STUDY; CYTOSKELETON; CYTOSOL; ESCHERICHIA COLI; HUMAN; IMMUNOPRECIPITATION; IN VITRO STUDY; NONHUMAN; PROTEIN ANALYSIS; PROTEOMICS; SCREENING; SENSITIVITY AND SPECIFICITY; SEROLOGY; TRYPANOSOMA RHODESIENSE; WESTERN BLOTTING; ANALYTICAL EQUIPMENT; BIOSYNTHESIS; BLOOD; CELL FRACTIONATION; CHEMISTRY; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PARASITOLOGY; PREDICTIVE VALUE; TRYPANOSOMIASIS;

TRYPANOSOMA BRUCEI; TRYPANOSOMA BRUCEI BRUCEI; TRYPANOSOMA BRUCEI RHODESIENSE;

CLONING, MOLECULAR; HISTONES; HSP70 HEAT-SHOCK PROTEINS; HUMANS; PHOSPHOGLYCERATE KINASE; PREDICTIVE VALUE OF TESTS; PROTEOMICS; REAGENT KITS, DIAGNOSTIC; SENSITIVITY AND SPECIFICITY; SEROLOGIC TESTS; SUBCELLULAR FRACTIONS; TRYPANOSOMA BRUCEI RHODESIENSE; TRYPANOSOMIASIS;

EID: 77949798151     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0009630     Document Type: Article

References (30)

1. Blum J, Nkunku S, Burri C (2001) Clinical description of encephalopathic syndromes and risk factors for their occurrence and outcome during melarsoprol treatment of human African trypanosomiasis. Trop Med Int Health 6: 390-400.
2. Burri C, Nkunku S, Merolle A, Smith T, Blum J, et al. (2000) Efficacy of new, concise schedule for melarsoprol in treatment of sleeping sickness caused by Trypanosoma brucei gambiense: a randomised trial. Lancet 355: 1419-1425.
3. Barrett M (2006) The rise and fall of sleeping sickness. Lancet 367: 1377-1378.
4. Chappuis F, Loutan L, Simarro P, Lejon V, Buscher P (2005) Options for field diagnosis of human african trypanosomiasis. Clin Microbiol Rev 18: 133-146.
5. Jannin J, Cattand P (2004) Treatment and control of human African trypanosomiasis. Curr Opin Infect Dis 17: 565-571.
6. Radwanska M, Chamekh M, Vanhamme L, Claes F, Magez S, et al. (2002) The serum resistance-associated gene as a diagnostic tool for the detection of Trypanosoma brucei rhodesiense. Am J Trop Med Hyg 67: 684-690.
7. Njiru Z, Mikosza A, Matovu E, Enyaru J, Ouma J, et al. (2008) African trypanosomiasis: Sensitive and rapid detection of the sub-genus Trypanozoon by loop-mediated isothermal amplification (LAMP) of parasite DNA. Int J Parasitol 38: 589-599.
8. Akol MN, Olaho-Mukani W, Odiit M, Enyaru JC, Matovu E, et al. (1999) Trypanosomosis agglutination card test for Trypanosoma brucei rhodesiense sleeping sickness. East Afr Med J 76: 38-41.
9. Desquesnes M, Kamyingkird K, Pruvot M, Kengradomkij C, Bossard G, et al. (2009) Antibody-ELISA for Trypanosoma evansi: application in a serological survey of dairy cattle, Thailand, and validation of a locally produced antigen. Prev Vet Med 90: 233-241.
10. Desquesnes M, Bossard G, Thévenon S, Patrel D, Ravel S, et al. (2009) Development and application of an antibody-ELISA to follow up a Trypanosoma evansi outbreak in a dromedary camel herd in France. Vet Parasitol 162: 214-220.
11. Hutchinson OC, Webb H, Picozzi K, Welburn S, Carrington M (2004) Candidate protein selection for diagnostic markers of African trypanosomiasis. Trends Parasitol 20: 519-523.
12. Muller N, Hemphill A, Imboden M, Duvallet G, Dwinger R, et al. (1992) Identification and characterization of two repetitive non-variable antigens from African trypanosomes which are recognized early during infection. Parasitology 104: 111-120.
13. Muller N, Imboden M, Detmer E, Mansfield JM, Seebeck T (1993) Cytoskeleton-associated antigens from African trypanosomes are recognized by self-reactive antibodies of uninfected mice. Parasitology 107: 411-417.
14. Umezawa E, Bastos S, Coura J, Levin M, Gonzalez A, et al. (2003) An improved serodiagnostic test for Chagas' disease employing a mixture of Trypanosoma cruzi recombinant antigens. Transfusion 43: 91-97.
15. Cooley G, Etheridge R, Boehlke C, Bundy B, Weatherly D, et al. (2008) High Throughput Selection of Effective Serodiagnostics for Trypanosoma cruzi infection. PLoS Negl Trop Dis 2: e316.
16. Nantulya V (1997) TrypTect CIATT-a card indirect agglutination trypanosomiasis test for diagnosis of Trypanosoma brucei gambiense and T. b. rhodesiense infections. Trans R Soc Trop Med Hyg 91: 551-553.
17. Asonganyi T, Doua F, Kibona S, Nyasulu Y, Masake R, et al. (1998) A multicentre evaluation of the card indirect agglutination test for trypanosomiasis (TrypTect CIATT). Ann Trop Med Parasitol 92: 837-844.
18. Forgber M, Basu R, Roychoudhury K, Theinert S, Roy S, et al. (2006) Mapping the Antigenicity of the Parasites in Leishmania donovani Infection by Proteome Serology. PLoS ONE 1: e40.
19. Gupta SK, Sisodia BS, Sinha S, Hajela K, Naik S, et al. (2007) Proteomic approach for identification and characterization of novel immunostimulatory proteins from soluble antigens of Leishmania donovani promastigotes. Proteomics 7: 816-823.
20. Maina NW, Oberle M, Otieno C, Kunz C, Maeser P, et al. (2007) Isolation and propagation of Trypanosoma brucei gambiense from sleeping sickness patients in south Sudan. Trans R Soc Trop Med Hyg.
21. Clayton CE (1987) Import of fructose bisphosphate aldolase into the glycosomes of Trypanosoma brucei. J Cell Biol.
22. Opperdoes FR, Baudhuin P, Coppens I, De Rose C, Edwards SW, et al. (1984) Purification, morphometric analysis, and characterization of the glycosomes (microbodies) of the protozoan hemoflagellate Trypanosoma brucei. J Cell Biol 98: 1178-1184.
23. Clayton CE (1987) Import of fructose bisphosphate aldolase into the glycosomes of Trypanosoma brucei. J Cell Biol 105: 2649-2653.
24. Robinson D, Beattie P, Sherwin T, Gull K (1991) Microtubules, tubulin, and microtubule-associated proteins of trypanosomes. Methods Enzymol 196: 285-299.
25. Caffrey C, Hansell E, Lucas K, Brinen L, Alvarez Hernandez A, et al. (2001) Active site mapping, biochemical properties and subcellular localization of rhodesain, the major cysteine protease of Trypanosoma brucei rhodesiense. Mol Biochem Parasitol 118: 61-73.
26. Brockmöller J, Kamp R (1985) Two-dimensional polyacrylamide gel electrophoresis of ribosomal proteins in the nanogram range. Biol Chem Hoppe Seyler 366: 901-906.
27. Heukeshoven J, Dernick R (1988) Increased sensitivity for Coomassie staining of sodium dodecyl sulfate-polyacrylamide gels using PhastSystem Development Unit. Electrophoresis 9: 60-61.
28. Landfear SM, Ullman B, Carter NS, Sanchez MA (2004) Nucleoside and Nucleobase Transporters in Parasitic Protozoa. Euk Cell 3: 245-254.
29. Lalmanach G, Boulangé A, Serveau C, Lecaille F, Scharfstein J, et al. (2002) Congopain from Trypanosoma congolense: drug target and vaccine candidate. Biol Chem 383: 739-749.
30. Flechas I, Cuellar A, Cucunubá Z, Rosas F, Velasco V, et al. (2009) Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients. BMC Infect Dis 9: 186.