Separation of recombinant β-glucuronidase from transgenic tobacco by aqueous two-phase extraction

Biochemical Engineering Journal

Volumn 49, Issue 3, 2010, Pages 343-350

  Ross, Kristin C ^a   Zhang, Chenming ^a  

^a State University   (United States)

Author keywords

Aqueous two phase extraction; Aqueous two phase system; Downstream processing; Recombinant glucuronidase; Recombinant protein; Transgenic tobacco

Indexed keywords

AQUEOUS TWO PHASE SYSTEM; AQUEOUS TWO-PHASE EXTRACTION; DOWNSTREAM-PROCESSING; GLUCURONIDASE; RECOMBINANT PROTEIN; TRANSGENIC TOBACCO;

IONIC STRENGTH; PLANTS (BOTANY); POLYETHYLENE GLYCOLS; POTASSIUM; PROTEINS; PURIFICATION; TOBACCO;

SOLVENT EXTRACTION;

BETA GLUCURONIDASE; PHOSPHATE; POTASSIUM; RECOMBINANT BETA GLUCURONIDASE; RECOMBINANT PROTEIN; RIBULOSEBISPHOSPHATE CARBOXYLASE; UNCLASSIFIED DRUG;

AQUEOUS TWO PHASE EXTRACTION; ARTICLE; DRUG SCREENING; ECONOMICS; EXTRACTION; FACTORIAL DESIGN; HOST; INTERPHASE; IONIC STRENGTH; NONHUMAN; PRIORITY JOURNAL; PURIFICATION; RESPONSE SURFACE METHOD; TOBACCO; TRANSGENIC PLANT;

NICOTIANA TABACUM;

EID: 77949490189     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2010.01.009     Document Type: Article

References (41)

1. Larrick J.W., Yua L., Naftzgera C., Jaiswala S., and Wycoffa K. Production of secretory IgA antibodies in plants. Biomol. Eng. 18 3 (2001) 87-94
2. Fischer R., and Emans N. Molecular farming of pharmaceutical proteins. Transgenic Res. 9 (2000) 279-299
3. Fischer R., Stoger E., Schillberg S., Christou P., and Twyman R.M. Plant-based production of biopharmaceuticals. Curr. Opin. Plant Biol. 7 (2004) 1-7
4. Menkhaus T.J., Bai Y., Zhang C., Nikolov Z.L., and Glatz C.E. Considerations for the recovery of recombinant proteins from plants. Biotechnol. Prog. 20 (2004) 1001-1014
5. Davis D.L., and Nielson M.T. Tobacco: Production, chemistry and technology (1999), Blackwell Science, Oxford
6. Cheryan M. Phytic acid interactions in food systems. Crit. Rev. Food Sci. Nutr. 13 4 (1980) 297-335
7. Jervis L., and Pierpoint W.S. Purification technologies for plant-proteins. J. Biotechnol. 11 2-3 (1989) 161-198
8. Witcher D.R., Hood E.E., Peterson D., Bailey M., Bond D., Kusnadi A., Evangelista R., Nikolov Z., Wooge C., Mehigh R., Kappe W., Register J., and Howard J.A. Commercial production of beta-glucuronidase (GUS): a model system for the production of proteins in plants. Mol. Breeding 4 4 (1998) 301-312
9. S.J. Garger, R.B. Holtz, M.J. McCulloch, T.H. Turpen, Process for isolating and purifying viruses, soluble proteins, and peptides from plant sources, U.S. Patent 6,033,895, 2000.
10. Kung S.D. Tobacco fraction 1 protein: a unique genetic marker. Science 191 4226 (1976) 429-434
11. Holler C., Vaughan D., and Zhang C. Polyethyleneimine precipitation versus anion exchange chromatography in fractionating recombinant β-glucuronidase from transgenic tobacco extract. J. Chromatogr. A 1142 (2007) 98-105
12. Kusnadi A.R., Nikolov Z.L., and Howard J.A. Production of recombinant proteins in transgenic plants: practical considerations. Biotechnol. Bioeng. 56 5 (1997) 473-484
13. Spieß A.C., Eberhard W., Peters M., Eckstein M.F., Greiner L., and Büchs J. Prediction of partition coefficients using COSMO-RS: solvent screening for maximum conversion in biocatalytic two-phase reaction systems. Chem. Eng. Process. 47 (2008) 1034-1041
14. Kroner K.H., Schutte H., Stach W., and Kula M.-R. Scale up of formate dehydrogenase by partition. J. Chem. Technol. Biotechnol. 32 (1982) 130-137
15. Hart R.A., Lester P.M., Riefsnyder H., Ogez J.R., and Builder S.E. Large scale, in situ isolation of periplasmic IGF-I from E. coli. Biotechnology 12 (1994) 1113-1117
16. X. Li, J. Wan, X. Cao, Preliminary application of light-pH sensitive recycling aqueous two-phase systems to purification of lipase, Process. Biochem., in press.
17. Silva M.E., and Franco T.T. Liquid-liquid extraction of biomolecules in downstream processing-a review paper. Braz. J. Chem. Eng. 17 1 (2000) 1-17
18. Salabat. The influence of salts on the phase compositions in aqueous two-phase systems: experiments and predictions. Fluid Phase Equilibr. 187-188 (2001) 489-498
19. van Berlo M., Luyben K.C.A.M., and van der Wielen L.A.M. Poly(ethylene glycol)-salt aqueous two-phase systems with easily recyclable volatile salts. J. Chromatogr. B: Biomed. Sci. Appl. 711 1-2 (1998) 61-68
20. Marcos J.C., Fonseca L.P., Ramalho M.T., and Cabral J.M. Partial purification of penicillin acylase from Escherichia coli in poly(ethylene glycol)-sodium citrate aqueous two-phase systems. J. Chromatogr. B: Biomed. Sci. Appl. 734 1 (1999) 15-22
21. Azevedo A.M., Rosa P.A., Ferreira I.F., and Aires-Barros M.R. Optimization of aqueous two-phase extraction of human antibodies. J. Biotechnol. 132 2 (2007) 209-217
22. Gu Z., and Glatz C.E. Aqueous two-phase extraction for protein recovery from corn extracts. J. Chromatogr. B 845 1 (2007) 38-50
23. Zhang C., Medina-Bolivarb F., Buswell S., and Cramer C.L. Purification and stabilization of ricin B from tobacco hairy root culture medium by aqueous two-phase extraction. J. Biotechnol. 117 1 (2005) 39-48
24. Balasubramaniam D., Wilkinson C., Van Cott K., and Zhang C. Tobacco protein separation by aqueous two-phase extraction. J. Chromatogr. A 989 1 (2003) 119-129
25. Platis D., and Labrou N.E. Development of an aqueous two-phase partitioning system for fractionating therapeutic proteins from tobacco extract. J. Chromatogr. A 1128 (2006) 114-124
26. Jefferson R.A., Burgess S.M., and Hirsh D. β-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc. Natl. Acad. Sci. U.S.A. 83 (1986) 8447-8451
27. Gallagher S.R. GUS Protocols: Using the GUS Gene as a Reporter of Gene Expression (1992), Academic Press, San Diego, CA
28. Pessela B.C.C., Fuentes M., Mateo C., Munilla R., Carrascosa A.V., Fernandez-Lafuente R., and Guisan J.M. Purification and very strong reversible immobilization of large proteins on anionic exchangers by controlling the support and the immobilization conditions. Enzyme Microb. Technol. 39 (2006) 909-915
29. Ni M., Cui D., Einstein J., Narasimhulu S., Vergara C.E., and Gelvin S.B. Strength and tissue specificity of chimeric promoters derived from the octopine and mannopine synthase genes. Plant J. 7 (1995) 661-676
30. Neve M.D., Houdt H.V., Bruyns H.-M., Montagu M.V., and Depicker A. Screening for transgenic lines with stable and suitable accumulation levels of a heterologous protein. In: Cunningham C., and Porter A.J.R. (Eds). Recombinant Proteins from Plants: Production and Isolation of Clinically Useful Compounds (1998), Humana Press, Totawa, NJ 203-227
31. Murashige T., and Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15 (1962) 473-497
32. Aich S., Delbaere L.T., and Chen R. Continuous spectrophotometric assay for β-glucuronidase. Biotechniques 30 (2001) 846-850
33. Jefferson R.A., and Wilson K.J. β-Glucuronidase as a gene fusion marker in agricultural molecular biology in plant molecular biology manual. In: Gelvin S., Schilperoort R., and Verma D.P. (Eds). Plant Molecular Biology Manual (1991), Kluwer Academic Publishers, Dordrecht, Netherlands 1-33
34. Holler C., and Zhang C. Purification of an acidic recombinant protein from transgenic tobacco. Biotechnol. Bioeng. 99 4 (2008) 902-909
35. Zaslavsky B.Y. Aqueous Two-phase Partitioning (1995), Marcel Dekker, Inc., New York
36. Albertsson P.A. Partition of Cell Particles and Macromolecules. second ed. (1986), John Wiley & Sons Inc., New York
37. Snyder S.M., Cole K.D., and Sziag D.C. Phase compositions, viscosities, and densities for aqueous two-phase systems composed of polyethylene glycol and various salts at 25 °C. J. Chem. Eng. Data 37 (1992) 268-274
38. Köhler K., Veide A., and Enfors S.-O. Partitioning of β-galactosidase fusion proteins in PEG-phosphate aqueous two-phase systems. Enzyme Microb. Technol. 13 (1991) 204-209
39. Kula M.-R., Kroner K.L., and Hustedt H. Purification of enzymes by liquid-liquid extraction. Adv. Biochem. Eng. 24 (1982) 73-118
40. Andrews B., and Asenjo J.A. Protein Purification Methods: A Practical Approach (1989), IRL Press, Oxford
41. Fernandez-Lafuente R. Stabilization of multimeric enzymes: strategies to prevent subunit dissociation. Enzyme Microb. Technol. 45 (2009) 405-418