Partial purification of penicillin acylase from Escherichia coli in poly(ethylene glycol)-sodium citrate aqueous two-phase systems

Journal of Chromatography B: Biomedical Sciences and Applications

Volumn 734, Issue 1, 1999, Pages 15-22

  Marcos, J C ^a   Fonseca, L P ^b   Ramalho, M T ^a   Cabral, J M S ^b  

^a UNIVERSITY OF MINHO   (Portugal)

^b INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

Aqueous two phase systems; Partitioning; Penicillin acylase; Poly(ethylene glycol) sodium citrate

Indexed keywords

CITRATE SODIUM; MACROGOL; PENICILLIN AMIDASE; SODIUM CHLORIDE;

ANALYTIC METHOD; AQUEOUS SOLUTION; ARTICLE; ENZYME PURIFICATION; ESCHERICHIA COLI; HYDROPHOBICITY; MOLECULAR WEIGHT; NONHUMAN; PARTITION COEFFICIENT; PH; PRIORITY JOURNAL;

CHEMISTRY, PHYSICAL; CITRATES; COLD; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; INDICATORS AND REAGENTS; MOLECULAR WEIGHT; OSMOTIC PRESSURE; PENICILLIN AMIDASE; PHOSPHATES; POLYETHYLENE GLYCOLS; SODIUM CHLORIDE;

EID: 0032877729     PISSN: 13872273     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-4347(99)00319-9     Document Type: Article

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