Tissue-specific knockout of TSHr in white adipose tissue increases adipocyte size and decreases TSH-induced lipolysis

Biochemical and Biophysical Research Communications

Volumn 393, Issue 3, 2010, Pages 526-530

  Elgadi, Aziz ^a   Zemack, Helen ^a   Marcus, Claude ^a   Norgren, Svante ^b  

^a KAROLINSKA INSTITUTE   (Sweden)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Adipocyte; Cre lox recombinant; Thyroid stimulating hormone receptor (TSHr); Tissue specific knockout

Indexed keywords

CATECHOLAMINE; INSULIN; THYROTROPIN; THYROTROPIN RECEPTOR;

ADIPOCYTE; ANIMAL TISSUE; ARTICLE; CELL METABOLISM; CONTROLLED STUDY; HISTOLOGY; KNOCKOUT GENE; LIPOLYSIS; LOXP SITE; MALE; MOUSE; NEWBORN; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; THYROID FUNCTION; THYROID GLAND; WHITE ADIPOSE TISSUE;

ADIPOCYTES; ADIPOSE TISSUE, WHITE; ANIMALS; CELL SIZE; LIPOLYSIS; MALE; MICE; MICE, KNOCKOUT; RECEPTORS, THYROTROPIN; THYROTROPIN;

ANIMALIA; MUS;

EID: 77949328379     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.02.042     Document Type: Article

References (43)

1. Kershaw E.E., and Flier J.S. Adipose tissue as an endocrine organ. J. Clin. Endocrinol. Metab. 89 (2004) 2548-2556
2. Rajala M.W., and Scherer P.E. Minireview: the adipocyte - at the crossroads of energy homeostasis, inflammation, and atherosclerosis. Endocrinology 144 (2003) 3765-3773
3. Qatanani M., and Lazar M.A. Mechanisms of obesity-associated insulin resistance: many choices on the menu. Genes Dev. 21 (2007) 1443-1455
4. Carmen G.Y., and Victor S.M. Signalling mechanisms regulating lipolysis. Cell Signal. 18 (2006) 401-408
5. Arner P., and Langin D. The role of neutral lipases in human adipose tissue lipolysis. Curr. Opin. Lipidol. 18 (2007) 246-250
6. Arner P. Human fat cell lipolysis: biochemistry, regulation and clinical role. Best Pract. Res. Clin. Endocrinol. Metab. 19 (2005) 471-482
7. Tavernier G., Barbe P., Galitzky J., Berlan M., Caput D., Lafontan M., and Langin D. Expression of beta3-adrenoceptors with low lipolytic action in human subcutaneous white adipocytes. J. Lipid Res. 37 (1996) 87-97
8. Ohisalo J.J., Ranta S., and Huhtaniemi I.T. Attenuated adenosine R-site effect in adipocytes in obesity. Metabolism 35 (1986) 143-146
9. Davies J.I., and Souness J.E. The mechanisms of hormone and drug actions on fatty acid release from adipose tissue. Rev. Pure Appl. Pharmacol. Sci. 2 (1981) 1-112
10. Marcus C., Karpe B., Bolme P., Sonnenfeld T., and Arner P. Changes in catecholamine-induced lipolysis in isolated human fat cells during the first year of life. J. Clin. Invest. 79 (1987) 1812-1818
11. Castan I., Valet P., Quideau N., Voisin T., Ambid L., Laburthe M., Lafontan M., and Carpene C. Antilipolytic effects of alpha 2-adrenergic agonists, neuropeptide Y, adenosine, and PGE1 in mammal adipocytes. Am. J. Physiol. 266 (1994) R1141-R1147
12. Lafontan M., and Berlan M. Fat cell alpha 2-adrenoceptors: the regulation of fat cell function and lipolysis. Endocr. Rev. 16 (1995) 716-738
13. Lafontan M., Bousquet-Melou A., Galitzky J., Barbe P., Carpene C., Langin D., Berlan M., Valet P., Castan I., Bouloumie A., et al. Adrenergic receptors, fat cells: differential recruitment by physiological amines and homologous regulation. Obes. Res. 4 Suppl. 3 (1995) 507S-514S
14. Langin D. Adipose tissue lipolysis as a metabolic pathway to define pharmacological strategies against obesity and the metabolic syndrome. Pharmacol. Res. 53 (2006) 482-491
15. Marcus C., Ehren H., Bolme P., and Arner P. Regulation of lipolysis during the neonatal period. Importance of thyrotropin. J. Clin. Invest. 82 (1988) 1793-1797
16. Janson A., Karlsson F.A., Micha-Johansson G., Bolme P., Bronnegard M., and Marcus C. Effects of stimulatory and inhibitory thyrotropin receptor antibodies on lipolysis in infant adipocytes. J. Clin. Endocrinol. Metab. 80 (1995) 1712-1716
17. Drvota V., Janson A., Norman C., Sylven C., Haggblad J., Bronnegard M., and Marcus C. Evidence for the presence of functional thyrotropin receptor in cardiac muscle. Biochem. Biophys. Res. Commun. 211 (1995) 426-431
18. Haraguchi K., Shimura H., Lin L., Saito T., Endo T., and Onaya T. Functional expression of thyrotropin receptor in differentiated 3T3-L1 cells: a possible model cell line of extrathyroidal expression of thyrotropin receptor. Biochem. Biophys. Res. Commun. 223 (1996) 193-198
19. Haraguchi K., Shimura H., Lin L., Endo T., and Onaya T. Differentiation of rat preadipocytes is accompanied by expression of thyrotropin receptors. Endocrinology 137 (1996) 3200-3205
20. Tsai J.A., Janson A., Bucht E., Kindmark H., Marcus C., Stark A., Zemack H.R., and Torring O. Weak evidence of thyrotropin receptors in primary cultures of human osteoblast-like cells. Calcif. Tissue Int. 74 (2004) 486-491
21. Marcus C., Sonnenfeld T., Karpe B., Bolme P., and Arner P. Inhibition of lipolysis by agents acting via adenylate cyclase in fat cells from infants and adults. Pediatr. Res. 26 (1989) 255-259
22. Marcus C., Bolme P., Karpe B., Bronnegard M., Sellden H., and Arner P. Expression of beta 1- and beta 2-receptor genes and correlation to lipolysis in human adipose tissue during childhood. J. Clin. Endocrinol. Metab. 76 (1993) 879-884
23. Nannipieri M., Cecchetti F., Anselmino M., Camastra S., Niccolini P., Lamacchia M., Rossi M., Iervasi G., and Ferrannini E. Expression of thyrotropin and thyroid hormone receptors in adipose tissue of patients with morbid obesity and/or type 2 diabetes: effects of weight loss. Int. J. Obes. (Lond.) 33 (2009) 1001-1006
24. Laugwitz K.L., Allgeier A., Offermanns S., Spicher K., Van Sande J., Dumont J.E., and Schultz G. The human thyrotropin receptor: a heptahelical receptor capable of stimulating members of all four G protein families. Proc. Natl. Acad. Sci. USA 93 (1996) 116-120
25. Stein S.A., Oates E.L., Hall C.R., Grumbles R.M., Fernandez L.M., Taylor N.A., Puett D., and Jin S. Identification of a point mutation in the thyrotropin receptor of the hyt/hyt hypothyroid mouse. Mol. Endocrinol. 8 (1994) 129-138
26. Beamer W.J., Eicher E.M., Maltais L.J., and Southard J.L. Inherited primary hypothyroidism in mice. Science 212 (1981) 61-63
27. Gu W.X., Du G.G., Kopp P., Rentoumis A., Albanese C., Kohn L.D., Madison L.D., and Jameson J.L. The thyrotropin (TSH) receptor transmembrane domain mutation (Pro556-Leu) in the hypothyroid hyt/hyt mouse results in plasma membrane targeting but defective TSH binding. Endocrinology 136 (1995) 3146-3153
28. Cordier A.C., Denef J.F., and Haumont S.M. Thyroid gland in dwarf mice: a stereological study. Cell Tissue Res. 171 (1976) 449-457
29. Li S., Crenshaw III E.B., Rawson E.J., Simmons D.M., Swanson L.W., and Rosenfeld M.G. Dwarf locus mutants lacking three pituitary cell types result from mutations in the POU-domain gene pit-1. Nature 347 (1990) 528-533
30. Kendall S.K., Samuelson L.C., Saunders T.L., Wood R.I., and Camper S.A. Targeted disruption of the pituitary glycoprotein hormone alpha-subunit produces hypogonadal and hypothyroid mice. Genes Dev. 9 (1995) 2007-2019
31. Rodbell M. Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis. J. Biol. Chem. 239 (1964) 375-380
32. Tchoukalova Y.D., Harteneck D.A., Karwoski R.A., Tarara J., and Jensen M.D. A quick, reliable, automated method for fat cell sizing. J. Lipid Res. 44 (2003) 1795-1801
33. Bjorkhem I., Arner P., Thore A., and Ostman J. Sensitive kinetic bioluminescent assay of glycerol release from human fat cells. J. Lipid Res. 22 (1981) 1142-1147
34. Johansson S.M., Salehi A., Sandstrom M.E., Westerblad H., Lundquist I., Carlsson P.O., Fredholm B.B., and Katz A. A1 receptor deficiency causes increased insulin and glucagon secretion in mice. Biochem. Pharmacol. 74 (2007) 1628-1635
35. Lu M., and Lin R.Y. TSH stimulates adipogenesis in mouse embryonic stem cells. J. Endocrinol. 196 (2008) 159-169
36. Ramsay T.G., Hausman G.J., and Martin R.J. Pre-adipocyte proliferation and differentiation in response to hormone supplementation of decapitated fetal pig sera. J. Anim. Sci. 64 (1987) 735-744
37. Hausman G.J., Hentges E.J., and Thomas G.B. Differentiation of adipose tissue and muscle in hypophysectomized pig fetuses. J. Anim. Sci. 64 (1987) 1255-1261
38. Hausman G.J., and Martin R.J. The influence of human growth hormone on preadipocyte development in serum-supplemented and serum-free cultures of stromal-vascular cells from pig adipose tissue. Domest. Anim. Endocrinol. 6 (1989) 331-337
39. Tsien J.Z., Chen D.F., Gerber D., Tom C., Mercer E.H., Anderson D.J., Mayford M., Kandel E.R., and Tonegawa S. Subregion- and cell type-restricted gene knockout in mouse brain. Cell 87 (1996) 1317-1326
40. Shao X., Somlo S., and Igarashi P. Epithelial-specific Cre/lox recombination in the developing kidney and genitourinary tract. J. Am. Soc. Nephrol. 13 (2002) 1837-1846
41. Engfeldt P., and Arner P. Lipolysis in human adipocytes, effects of cell size, age and of regional differences. Horm. Metab. Res. Suppl. 19 (1988) 26-29
42. Despres J.P., Fong B.S., Julien P., Jimenez J., and Angel A. Regional variation in HDL metabolism in human fat cells: effect of cell size. Am. J. Physiol. 252 (1987) E654-E659
43. Weyer C., Foley J.E., Bogardus C., Tataranni P.A., and Pratley R.E. Enlarged subcutaneous abdominal adipocyte size, but not obesity itself, predicts type II diabetes independent of insulin resistance. Diabetologia 43 (2000) 1498-1506