Weak Evidence of Thyrotropin Receptors in Primary Cultures of Human Osteoblast-like Cells

Calcified Tissue International

Volumn 74, Issue 5, 2004, Pages 486-491

  Tsai, J A ^a   Janson, A ^a   Bucht, E ^a   Kindmark, H ^b   Marcus, C ^a   Stark, A ^c   Zemack, H Rawet ^a   Torring, O ^c  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^b UPPSALA UNIVERSITY HOSPITAL   (Sweden)

^c KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Free cytoplasmic calcium; hTSHR; Human osteoblasts; Intracellular cAMP

Indexed keywords

CALCIUM; CYCLIC AMP; MESSENGER RNA; THYROTROPIN I 125; THYROTROPIN RECEPTOR;

ANIMAL CELL; ARTICLE; BONE METABOLISM; CALCIUM CELL LEVEL; CELL CULTURE; CELL STIMULATION; CONTROLLED STUDY; CYTOPLASM; GENE EXPRESSION; GENETIC TRANSFECTION; HORMONE RECEPTOR INTERACTION; HUMAN; HUMAN CELL; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RIBONUCLEASE PROTECTION ASSAY; SIGNAL TRANSDUCTION;

ANIMALS; BASE SEQUENCE; BONE AND BONES; CALCIUM; CELLS, CULTURED; CHO CELLS; CRICETINAE; CRICETULUS; CYCLIC AMP; HUMANS; OSTEOBLASTS; RATS; RECEPTORS, THYROTROPIN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; THYROTROPIN; TRANSFECTION;

EID: 2442505089     PISSN: 0171967X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00223-003-0108-3     Document Type: Article

References (27)

1. Endo T, Ohno M, Kotani S, Gunji K, Onaya T (1993) Thyrotropin receptor in non-thyroid tissues. Biochem Biophys Res Commun 190:774-779
2. Nagayama Y, Rapoport B (1992) The thyrotropin receptor 25 years after its discovery: new insight after its molecular cloning. Mol Endocrinol 6:145-156
3. Smith BR, Hall R (1974) Thyroid-stimulating immunoglobulins in Graves' disease. Lancet 2:427-431
4. Nagayama Y, Kaufman KD, Seto P, Rapoport B (1989) Molecular cloning, sequence and functional expression of the cDNA for the human thyrotropin receptor. Biochem Biophys Res Commun 165:1184-1190
5. Feliciello A, Porcellini A, Ciullo I, Bonavolonta G, Avvedimento EV, et al. (1993) Expression of thyrotropin-receptor mRNA in healthy and Graves' disease retro-orbital tissue. Lancet 342:337-338
6. Stadlmayr W, Spitzweg C, Bichlmair AM, Heufelder AE (1997) TSH receptor transcripts and TSH receptor-like immunoreactivity in orbital and pretibial fibroblasts of patients with Graves' ophthalmopathy and pretibial myxedema. Thyroid 7:3-12
7. Heufelder AE, Dutton CM, Sarkar G, Donovan KA, Bahn RS (1993) Detection of TSH receptor RNA in cultured fibroblasts from patients with Graves' ophthalmopathy and pretibial dermopathy. Thyroid 3:297-300
8. Agretti P, Chiovato L, De Marco G, Marcocci C, Mazzi B, et al. (2002) Real-time PCR provides evidence for thyrotropin receptor mRNA expression in orbital as well as in extraorbital tissues. Eur J Endocrinol 147:733-739
9. Marcus C, Ehren H, Bolme P, Arner P (1988) Regulation of lipolysis during the neonatal period. Importance of thyrotropin. J Clin Invest 82:1793-1797
10. Janson A, Karlsson FA, Micha-Johansson G, Bolme P, Bronnegard M (1995) Effects of stimulatory and inhibitory thyrotropin receptor antibodies on lipolysis in infant adipocytes. J Clin Endocrinol Metab 80:1712-1716
11. Janson A, Rawet H, Perbeck L, Marcus C (1998) Presence of thyrotropin receptor in infant adipocytes. Pediatr Res 43:555-558
12. Inoue M, Tawata M, Yokomori N, Endo T, Onaya T (1998) Expression of thyrotropin receptor on clonal osteoblast-like rat osteosarcoma cells. Thyroid 8:1059-1064
13. Tsai JA, Bucht E, Stark A, Sjostedt U, Torring O (1998) Parathyroid hormone-related protein (1-37) induces cAMP response in human osteoblast-like cells. Calcif Tissue Int 62:250-254
14. Tsai JA, Rong H, Matsushita H, Torring O, Bucht E (2000) Interleukin-1beta upregulate PTHrP-mRNA expression and protein production and decreases TRF-β in normal human osteoblast-like cells. Calcif Tissue Int 66:363-369
15. Torring O, Firek AF, Heath 3d H, Conover CA (1991) Parathyroid hormone and parathyroid hormone-related peptide stimulate insulin-like growth factor-binding protein secretion by rat osteoblast-like cells through a adenosine 3′,5′-monophosphate-dependent mechanism. Endocrinology 128:1006-1014
16. Van Sande J, Raspe E, Perret J, Lejeune C, Maenhaut C, et al. (1990) Thyrotropin activates both the cyclic AMP and the PIP2 cascades in CHO cells expressing the human cDNA of TSH receptor. Mol Cell Endocrinol 74:R1-6
17. 2+ in normal human osteoblast-like cells: aspects of their regulation. Biochem Biophys Res Commun pp 206-212
18. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159
19. Drvota V, Janson A, Norman C, Sylven C, Haggblad J, et al. (1995) Evidence for the presence of functional thyrotropin receptor in cardiac muscle. Biochem Biophys Res Commun 211:426-431
20. Dixon CJ, Bowler WB, Walsh CA, Gallagher JA (1997) Effects of extracellular nucleotides on single cells and populations of human osteoblasts: contribution of cell heterogeneity to relative potencies. Br J Pharmacol 120:777-780
21. Hidaka A, Minegishi T, Kohn LD (1993) Thyrotropin, like luteinizing hormone (LH) and chorionic gonadotropin (CG), increases cAMP and inositol phosphate levels in cells with recombinant human LH/CG receptor. Biochem Biophys Res Commun 196:187-195
22. Wakasugi M, Wakao R, Tawata M, Gan N, Koizumi K, et al. (1993) Bone mineral density in patients with hyperthyroidism measured by dual energy X-ray absorptiometry. Clin Endocrinol 38:283-286
23. Torring O, Tallstedt L, Wallin G, Lundell G, Ljunggren JG, et al. (1996) Graves' hyperthyroidism: treatment with antithyroid drugs, surgery, or radioiodine - a prospective, randomized study. Thyroid Study Group. J Clin Endocrinol Metab 81:2986-2993
24. Vestergaard P, Mosekilde L (2002) Fracture risk in patients with hyperthyroidism and hypothyroidism: a nation-wide study of 16,249 patients. Thyroid 12:411-419
25. Vestergaard P, Rejnmark L, Weeke J, Mosekilde L (2000) Fracture risk in patients treated for hyperthyroidism. Thyroid 10:341-348
26. Kumeda Y, Inaba M, Tahara H, Kurioka Y, Ishikawa T, et al. (2000) Persistent increase in bone turnover in Graves' patients with subclinical hyperthyroidism. J Clin Endocrinol Metab 85:4157-4161
27. Bauer DC, Nevitt MC, Ettinger B, Stone K (1997) Low thyrotropin levels are not associated with bone loss in older women: a prospective study. J Clin Endocrinol Metab 82:2931-2936