De novo synthesis, constitutive expression of Aspergillus sulphureus β-xylanase gene in Pichia pastoris and partial enzymic characterization

Applied Microbiology and Biotechnology

Volumn 76, Issue 3, 2007, Pages 579-585

  Cao, Yunhe ^a   Qiao, Jiayun ^a   Li, Yihang ^a   Lu, Wenqing ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Aspergillus sulphureus; Constitutive expression; De novo synthesis; Endo 1, 4 xylanase; Pichia pastoris

Indexed keywords

AMINO ACIDS; BIOSYNTHESIS; CATALYST ACTIVITY; FERMENTATION; GENE EXPRESSION;

ASPERGILLUS SULPHUREUS; CONSTITUTIVE EXPRESSION; DE NOVO SYNTHESIS; ENDO-Β-1, 4-XYLANASE; PICHIA PASTORIS;

BACTERIA;

BUFFER; CITRIC ACID; EDETIC ACID; ENDO 1,4 BETA XYLANASE; GLUCOSE; MATING HORMONE ALPHA FACTOR; METAL ION; NUCLEOTIDE; PEPTONE; RECOMBINANT ENZYME; SIGNAL PEPTIDASE; SYNTHETIC DNA;

AMINO ACID; DNA; ENZYME; FUNGUS; GENE EXPRESSION; PEPTIDE; PH; POLYMERASE CHAIN REACTION; PROTEIN;

ARTICLE; ASPERGILLUS; ASPERGILLUS SULPHUREUS; CODON; CONTROLLED STUDY; DIALYSIS MEMBRANE; ELECTROTRANSFORMATION; ENZYME ACTIVITY; ENZYME RELEASE; FERMENTATION MEDIUM; FUNGAL GENE; FUNGAL STRAIN; GENE EXPRESSION; GENE SYNTHESIS; LIQUID CULTURE; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; XYNA GENE; YEAST;

ASPERGILLUS; BASE SEQUENCE; BIOTECHNOLOGY; CLONING, MOLECULAR; DNA PRIMERS; DNA, FUNGAL; ENDO-1,4-BETA XYLANASES; ENZYME STABILITY; FERMENTATION; GENE EXPRESSION; GENES, FUNGAL; MOLECULAR SEQUENCE DATA; PICHIA; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, NUCLEIC ACID;

ASPERGILLUS SULPHUREUS; PICHIA PASTORIS;

EID: 34548012364     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-0978-9     Document Type: Article

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