Optimized expression of an acid xylanase from Aspergillus usamii in Pichia pastoris and its biochemical characterization

World Journal of Microbiology and Biotechnology

Volumn 24, Issue 8, 2008, Pages 1393-1401

  Zhou, Chenyan ^a   Li, Dongfeng ^a   Wu, Minchen ^a   Wang, Wu ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Aspergillus usamii; Characterization; Expression; Pichia pastoris; Xylanase; Xylooligosaccharides

Indexed keywords

HEALTH;

ALCOHOL OXIDASE (AOD); BIOCHEMICAL CHARACTERIZATIONS; ELECTROPORATION; METHYLOTROPHIC YEASTS; P. PASTORIS; PICHIA PASTORIS (PPPFK); XYLANASE;

BIOCHEMICAL ENGINEERING;

ASPERGILLUS; ASPERGILLUS USAMII; PICHIA PASTORIS;

EID: 45849137846     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-007-9622-1     Document Type: Article

References (45)

1. Akira M, Yumiko TA (2001) Comparison of three signals for secretory expression of recombinant human midkine in Pichia pastoris. Biosci Biotechnol Biochem 65:2291-2293
2. Baumgartner P, Raemaekers RJ, Durieux A, Gatehouse A, Davies H, Taylor M (2002) Large-scale production, purification, and characterization of recombinant Phaseolus vulgaris phytohemagglutinin E-form expressed in the methylotrophic yeast Pichia pastoris. Protein Expr Purif 26:394-405
3. Beg QK, Kapoor M, Mahajan L, Hoondal GS (2001) Microbial xylanases and their industrial applications: a review. Appl Microbiol Biotechnol 56:326-338
4. Belancic A, Scarpa J, Peirano A, Diaz R, Steiner J, Eyzayuirre J (1995) Penicillium purpurogenum produces several xylanases:purification and properties of two of the enzymes. J Biotechnol 41:71-79
5. Biely P (1985) Microbial xylanolytic enzymes. Trends Biotechnol 11:286-290
6. Collin T, Gerday C, Feller G (2005) Xylanase, xylanase families and extremophilic xylanases. FEMS Microbiol Rev 29:3-23
7. Damaso MC, Almedia MS, Kurtenbach E, Martins OB, Pereira NJ, Andrade CM (2003) Optimized expression of a thermostable xylanase from Thermomyces lanuginosus in Pichia pastoris. Appl Environ Microbiol 69:6064-6072
8. Delroisse JM, Marie D, Gilsoul JJ, Thami EM, Jacqueline D, Daniel P, Philippe T, Eric H, Micheline V (2005) Expression of a synthetic gene encoding a Tribolium castaneum carbosylesterase in Pichia pastoris. Protein Expr Purif 42:286-294
9. Farooq L, Muhammad A, Rabia S, Ahmed A, Legge RL (2006) Purification and characterization of a xylanase produced by Chaetomium thermophile NIBGE. World J Microbiol Biotechnol 22:45-50
10. Fu DD, Xie H, Wu MC (2005) Solid fermentation for xylanase production by Aspergillus usamii. Food Ferment Indus (Chin J) 31:50-53
11. Gorgenes JF, Van Zye WH, Kuoetze JH, Han-Hageral B (2005) Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium. Appl Microbiol Biotechnol 67:684-69l
12. Higgins DR, Busser K, Comiskey J, Whittier PS, Purcell TJ, Hoeffler JP (1998) Small vectors for expression based on dominant drug resistance with direct multicopy selection. In: Higgins DR, Cregg JM (eds) Methods in molecular biology: Pichia protocols. Humana Press, New Jersey, pp 28-41
13. Inagaki K, Nakahira K, Mukai K, Tamura T, Tanaka H (1998) Gene cloning and characterization of an acidic xylanase from Acidobacterium capsulatum. Biosci Biotechnol Biochem 62:1061-1067
14. Jesus MP, Maria JB, Xin GL (2002) Functional expression of keratinase (kerA) gene from Bacillus licheniformis in Pichia pastoris. Biotechnol Lett 24:631-636
15. Kimura I, Sasahar H, Tajima S (1995) Purification and characterization of two xylanases and an arabinofuranosidase from Aspergillus sojae. J Ferment Bioeng 80:334-339
16. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
17. Laetitia B, Virginie N, Laure L, Helene B, Guy M, Frederic B, Eric D (2004) High-level expression of Candida parapsilosis lipase/acyltransferase in Pichia pastoris. J Biotechnol 111:41-50
18. Layla F, Ignacio PV, Alberto Z, Pedro LB, Juan CM, Javier V, Jose LA (2006) High-level expression and characterization of Galactomyces geotrichum (BT107) lipase I in Pichia pastoris. Protein Expr Purif 49:256-264
19. Manuel M, Cecilia C, Lester H, Jose P, Laura V, Jorge V, Yanet T, Abdel H, Liuba P, Vladimir B, Liudys G, Emilio L, Lila C, Eduardo M (2005) Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris. Biotechnol Lett 27:339-345
20. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 31:426-428
21. Nicolas P, Dieter K, Francois S, Rolf M (1996) Increased xylanase yield in Streptomyces lividans:dependence on number of ribosome-binding sites. Nat Biotechnol 14:756-759
22. Polizeli MLTM, Rizzattim ACS, Monti R, Terenzi HF, Jorge JA, Amorim DS (2005) Xylanases from fungi: properties and industrial applications. Appl Microbiol Biotechnol 67:577-591
23. Radionova NA, Dubovaya NV, Eneiskaya EV, Matrinovich LI, Gracheva IM, Bezborodov AM (2000) Purification and characterization of endo-1,4-β- xylanase from Geotrichum candidum 3C. Appl Biochem Microbiol 36:460-465
24. Raj KC, Chandra TS (1996) Purification and characterization of xylanase from alkalitolerant Aspergillus fischeri Fxn 1. FEMS Microbiol Lett 145:457-461
25. Rose SH, Van Zyl W (2002) Constitutive expression of the Trichoderma reesei beta-1,4-xylanase gene (xyn2) and the beta-1,4-entoglucanase gene (egl) in Aspergillus niger in molasses and definded glucose media. Appl Microbiol Biotechnol 58:461-468
26. Ruiz-Arribas A, Fernandez-Abalos JM, Sanchez P, Garda AL, Santamaria RI (1997) Overproduction, purification, and biochemical characterization of a xylanase (Xysl) from Streptomyces halstedii JM8. Appl Environ Microbiol 61:2414-2419
27. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2 edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
28. Sapre MP, Jha H, Patil MB (2005) Purification and characterization of a thermoalkaliphilic xylanase from Bacillus sp. World J Microbiol Biotechnol 21:649-654
29. Shah AR, Madamwar D (2005) Xylanase production by a newly isolated Aspergillus foetidus strain and its characterization. Process Biochem 40:1763-1771
30. Shao W, Wiegel J (1992) Purification and characterization of a thermostable β-xylosidase from Thermoanaerobacter ethanolicus. J Bacteriol 174:5848-5853
31. Sharma A, Adhikari S, Satyanarayana T (2007) Alkali-thermostable and cellulase-free xylanase production by an extreme thermophile Geobacillus thermoleovorans. World J Microbiol Biotechnol 23:483-490
32. Subramaniyan S, Prema P (2002) Biotechnology of microbial xylanases: enzymology, molecular biology, and application. Crit Rev Biotechnol 22:33-64
33. Sue MP, Mariana LF, Brian M, Linda MH (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast 22:249-270
34. Techapun C, Poosaran N, Watanabe M, Sasaki K (2003) Thermostable and alkaline-tolerant microbial cellulose-free xylanases produced from agricultural wastes and the properties required for use in pulp bleaching bioprocesses: a review. Process Biochem 38:1327-1340
35. Vasimon R, Rutchadaporn S, Nakul R, Pacawadee T, Duriya C, Sutipa T, Kusol P, Lily E (2007) Cloning, expression, characterization, and high cell-density production of recombinant endo-1,4-β-xylanase from Aspergillus niger in Pichia pastoris. Enzyme Microb Technol 41:19-25
36. Viikari L, Kantelinen A, Sundquist J, Linko M (1994) Xylanases in bleaching: from an idea to the industry. FEMS Microbiol Rev 13:335-350
37. Villatte F, Hussein AS, Bachmann TT (2001) Expression level of heterologous protein in Pichia pastoris is influenced by flask design. Appl Microbiol Biotechnol 55:463-465
38. Wang Q, Xia T (2007) Enhancement of the thermostability and hydrolytic activity of GH10 xylanase by module shuffling between Cellulomonas fimi Cex and Thermomonospora alba XylA. World J Microbiol Biotechnol 23:1047-1055
39. Wang YR, Zhang HL, He YZ, Luo HY, Yao B (2007) Characterization, gene cloning, and expression of a novel xylanase XYNB from Streptomyces olivaceoviridis Al. Aquaculture 267:328-334
40. Wong KKY, Saddler JN (1993) Applications of hemicellulases in the food, feed and pulp and paper industries. In: Coughlan MP, Hazlewood GP (eds) Hemicellulose and hemicellulases. Portland Press, London, pp 127-143
41. Wu MC, Fu DD, Zhu J, Xia MF (2005) Purification and some properties of two xylanases from Aspergillus usamii. J Food Sci Biotechnol (Chin J) 24:29-33
42. Xiong H, Weymarn N, Leisola M, Turunen O (2004) Influence of pH on the production of xylanase by Trichoderma reesei C-30. Process Biochem 39:731-736
43. Xu ZH, Bai YL, Xu X, Shi JS, Tao WY (2005) Production of alkali-tolerant cellulase-free xylanase by Pseudomonas sp. WLUN024 with wheat bran as the main substrate. World J Microbiol Biotechnol 21:575-581
44. Zhijian S, Xiaoping W, Ya F, Changcai D, Yechen X, Lu C, Wenke F, Xiaokun L (2007) High level expression of human endostatin in Pichia pastoris using a synthetic gene construct. Appl Microbiol Biotechnol 73:1355-1362
45. Zhou CY, Bai JY, Deng SS, Wang J, Zhu J, Wu MC, Wang W (2008) Cloning of a xylanase gene from Aspergillus usamii and its expression in Escherichia coli. Bioresour Technol 99:831-838