Squalene-based oil-in-water emulsion adjuvants perturb metabolism of neutral lipids and enhance lipid droplet formation

Biochemical and Biophysical Research Communications

Volumn 393, Issue 3, 2010, Pages 350-355

  Kalvodova, Lucie ^a  

^a INFECTIOUS DISEASE RESEARCH INSTITUTE   (United States)

Author keywords

Acylglycerols; Cholesteryl ester; Lipid droplets; Lipid metabolism; Oil in water emulsion adjuvants; Squalene

Indexed keywords

CHOLESTEROL ESTER; DIACYLGLYCEROL; FAT DROPLET; FATTY ACID; IMMUNOLOGICAL ADJUVANT; LIPID; LIPOSOME; POLOXAMER; SQUALENE; TRIACYLGLYCEROL; WATER OIL CREAM;

ANIMAL CELL; ARTICLE; CELL METABOLISM; CELL TYPE; CONTROLLED STUDY; CYTOPLASM; DENDRITIC CELL; DRUG DELIVERY SYSTEM; ENDOCYTOSIS; FIBROBLAST; INTERNALIZATION; LIPID METABOLISM; MACROPHAGE; MONOCYTE; MOUSE; MUSCLE CELL; NONHUMAN; PRIORITY JOURNAL;

ADJUVANTS, IMMUNOLOGIC; ANIMALS; CELL LINE; EMULSIONS; ENDOCYTOSIS; FIBROBLASTS; LIPID METABOLISM; MICE; MUSCLE FIBERS, SKELETAL; OILS; PHAGOCYTES; SQUALENE; VACCINES; WATER;

EID: 77949264892     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.12.062     Document Type: Article

References (30)

1. Higgins D., Carlson J., and Van Nest G. MF59 adjuvant enhances the immunogenicity of influenza vaccine in both young and old mice. Vaccine 14 (1996) 478-484
2. Ott G., Barchfeld G., and Van Nest G. Enhancement of humoral response against human influenza vaccine with the simple submicron oil/water emulsion adjuvant MF59. Vaccine 13 (1995) 1557-1562
3. Allison A. Squalene and squalane emulsions as adjuvants. Methods 19 (1999) 87-93
4. Yang Y.W., and Shen S.S. Enhanced antigen delivery via cell death induced by the vaccine adjuvants. Vaccine 25 (2007) 7763-7772
5. Dupuis M., McDonald D., and Ott G. Distribution of adjuvant MF59 and antigen gD2 after intramuscular injection in mice. Vaccine 18 (1999) 434-439
6. Seubert A., Monaci E., Pizza M., O'Hagan D., and Wack A. The adjuvants aluminum hydroxide and MF59 induce monocyte and granulocyte chemoattractants and enhance monocyte differentiation toward dendritic cells. J. Immunol. 180 (2008) 5402-5412
7. Dupuis M., Denis-Mize K., LaBarbara A., Peters W., Charo I., McDonald D., and Ott G. Immunization with the adjuvant MF59 induces macrophage trafficking and apoptosis. Eur. J. Immunol. 31 (2001) 2910-2918
8. Mosca F., Tritto E., Muzzi A., Monaci E., Bagnoli F., Iavarone C., O'Hagan D., Rappuoli R., and De Gregorio E. Molecular and cellular signatures of human vaccine adjuvants. PNAS 105 (2008) 10501-10506
9. Björkbacka H., Kunjathoor V., Moore K., Koehn S., Ordija C., Lee M., Means T., Halmen K., Luster A., Golenbock D., and Freeman M. Reduced atherosclerosis in MyD88-null mice links elevated serum cholesterol levels to activation of innate immunity signaling pathways. Nat. Med. 10 (2004) 416-421
10. Pacheco P., Bozza F., Gomes R., Bozza M., Weller P., Castro-Faria-Neto H., and Bozza P. Lipopolysaccharide-induced leukocyte lipid body formation in vivo: innate immunity elicited intracellular loci involved in eicosanoid metabolism. J. Immunol. 169 (2002) 6498-6506
11. D'Avila H., Maya-Monteiro C., and Bozza P. Lipid bodies in innate immune response to bacterial and parasite infections. Int. Immunopharmacol. 8 (2008) 1308-1315
12. Bozza P., Magalhaes K., and Weller P. Leukocyte lipid bodies - biogenesis and functions in inflammation. Biochim. Biophys. Acta 1791 (2009) 540-551
13. Fox C., Anderson R., Dutill T., Goto Y., Reed S., and Vedvick T. Monitoring the effects of component structure and source on formulation stability and adjuvant activity of oil-in-water emulsions. Colloids Surf. B Biointerfaces 65 (2008) 98-105
14. Tangirala R., Mahlberg F., Glick J., Jerome W., and Rothblat G. Lysosomal accumulation of unesterified cholesterol in model macrophage foam cells. J. Biol. Chem. 268 (1993) 9653-9660
15. Grimard V., Massier J., Richter D., Schwidke D., Kalaidzidis Y., Fava E., Hermetter A., and Thiele C. SiRNA screening reveals JNK2 as an evolutionary conserved regulator of triglyceride homeostasis. J. Lipid Res. 49 (2008) 2427-2440
16. Yao J. Metabolic turnover of fatty acids and acylglycerols in rat sciatic nerve. J. Neurochem. 45 (1985) 589-595
17. Dupuis M., Murphy T., Higgins D., Ugozzoli M., van Nest G., Ott G., and McDonald D. Dendritic cells internalize vaccine adjuvant after intramuscular injection. Cell. Immunol. 186 (1998) 18-27
18. van Tellingen O., Beijnen J., Verweij J., Scherrenburg E., Nooijen W., and Sparreboom A. Rapid esterase-sensitive breakdown of polysorbate 80 and its impact on the plasma pharmacokinetics of docetaxel and metabolites in mice. Clin. Cancer Res. 5 (1999) 2918-2924
19. Carvalho M., Harada L., Gidlund M., Ketelhuth D., Boschcov P., and Quintao E. Macrophages take up triacylglycerol-rich emulsions at a faster rate upon co-incubation with native and modified LDL: an investigation on the role of natural chylomicrons in atherosclerosis. J. Cell. Biochem. 84 (2002) 309-323
20. Daher C.F., Baroody G., and Howland R. Effect of a surfactant, Tween 80, on the formation and secretion of chylomicrons in the rat. Food Chem. Toxicol. 41 (2003) 575-582
21. Plou F., Ferrer M., Nuero O., Calvo M., Alcalde M., Reyes F., and Ballesteros A. Analysis of Tween 80 as an esterase/lipase substrate for lipolytic activity assay. Biotechnol. Tech. 12 (1998) 183-186
22. Listenberger L., Han X., Lewis S., Cases S., Farese R., Ory D., and Schaffer J. Triglyceride accumulation protects against fatty acid-induced lipotoxicity. Proc. Natl. Acad. Sci. USA 100 (2003) 3077-3082
23. Hodgkin M., Pettitt T., Martin A., Michell R., Pemberton A., and Wakelam M. Diacylglycerols and phosphatidates: which molecular species are intracellular messengers?. Trends Biochem. Sci. 23 (1998) 200-204
24. Murphy R., James P., McAnoy A., Krank J., Duchoslav E., and Barkley R. Detection of the abundance of diacylglycerol and triacylglycerol molecular species in cells using neutral loss mass spectrometry. Anal. Biochem. 366 (2007) 59-70
25. Prieur X., Röszer T., and Ricote M. Lipotoxicity in macrophages: evidence from diseases associated with the metabolic syndrome. BBA - Mol. Cell Biol. Lipids 1801 (2010) 327-337
26. Cox B., Griffin E., Ullery J., and Jerome W. Effects of cellular cholesterol loading on macrophage foam cell lysosome acidification. J. Lipid Res. 48 (2007) 1012-1021
27. Boya P., and Kroemer G. Lysosomal membrane permeabilization in cell death. Oncogene 27 (2008) 6434-6451
28. Hornung V., Bauernfeind F., Halle A., Samstad E., Kono H., Rock K., Fitzgerald K., and Latz E. Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization. Nat. Immunol. 9 (2008) 847-856
29. Schweitzer S., Reding A., Patton H., Sullivan T., Stubbs C., Villalobos-Menuey E., Huber S., and Newell M. Endogenous versus exogenous fatty acid availability affects lysosomal acidity and MHC class II expression. J. Lipid Res. 47 (2006) 2525-2537
30. Zhou Q., Leeman S., and Amar S. Signaling mechanisms involved in altered function of macrophages from diet-induced obese mice affect immune responses. PNAS 106 (2009) 10740-10745