Absence of the aflatoxin biosynthesis gene, norA, allows accumulation of deoxyaflatoxin B1 in Aspergillus flavus cultures

FEMS Microbiology Letters

Volumn 305, Issue 1, 2010, Pages 65-70

  Ehrlich, Kenneth C ^a   Chang, Perng Kuang ^a   Scharfenstein Jr , Leslie L ^a   Cary, Jeffrey W ^a   Crawford, Jason M ^b   Townsend, Craig A ^b  

^a SOUTHERN REGIONAL RESEARCH CENTER   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

Aflatoxicol; Aflatoxin biosynthesis; Aryl alcohol dehydrogenase; Aspergillus flavus; Gene disruption; MS

Indexed keywords

AFLATOXICOL; AFLATOXIN; AFLATOXIN B1; ARYLALCOHOL DEHYDROGENASE; DEOXYAFLATOXIN B1; OXIDOREDUCTASE; UNCLASSIFIED DRUG;

ACIDITY; ARTICLE; ASPERGILLUS FLAVUS; BIOSYNTHESIS; CONTROLLED STUDY; CULTURE MEDIUM; FUNGAL GENE; FUNGUS CULTURE; FUNGUS MUTANT; NONHUMAN; NORA GENE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; WILD TYPE;

AFLATOXINS; ALCOHOL DEHYDROGENASE; ASPERGILLUS FLAVUS; BIOSYNTHETIC PATHWAYS; FUNGAL PROTEINS; GENE KNOCKOUT TECHNIQUES; GENES, FUNGAL; MULTIGENE FAMILY; OXIDATION-REDUCTION;

ASPERGILLUS; ASPERGILLUS FLAVUS;

EID: 77749328042     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.01914.x     Document Type: Article

References (22)

1. Bhatnagar D, McCormick SP Lee LS (1987a) Averufanin is an aflatoxin B1 precursor between averantin and averufin in the biosynthetic pathway. Appl Environ Microb 53 : 14 16.
2. Bhatnagar D, McCormick SP, Lee LS Hill RA (1987b) Identification of O-methylsterigmatocystin as an aflatoxin B1 and G1 precursor in Aspergillus parasiticus. Appl Environ Microb 53 : 1028 1033. (Pubitemid 17068249)
3. Brown DW, Yu JH, Kelkar HS et al 1996) Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans. P Natl Acad Sci USA 93 : 1418 1422.
4. Cary JW, Wright M, Bhatnagar D, Lee R Chu FS (1996) Molecular characterization of an Aspergillus parasiticus gene, norA, located on the aflatoxin biosynthesis gene cluster. Appl Environ Microb 62 : 360 366. (Pubitemid 26051560)
5. Chang P-K, Ehrlich KC, Linz JE, Bhatnagar D, Cleveland TE Bennett JW (1996) Characterization of the Aspergillus parasiticus niaD and niiA gene cluster. Curr Genet 30 : 68 75.
6. Chatterjee M Townsend CA (1994) Evidence for the probable final steps in aflatoxin biosynthesis. J Org Chem 59 : 4424 4429.
7. Chauvaux S, Chevalier F, Le Dantec C, Fayolle F, Miras I, Kunst F Beguin P (2001) Cloning of a genetically unstable cytochrome P-450 gene cluster involved in degradation of the pollutant ethyl tert-butyl ether by Rhodococcus ruber. J Bacteriol 183 : 6551 6557.
8. Ehrlich KC, Chang P-K, Yu J Cotty PJ (2004) Aflatoxin biosynthesis cluster gene cypA is required for G aflatoxin formation. Appl Environ Microb 70 : 6518 6524.
9. Ehrlich KC, Yu J Cotty PJ (2005) Aflatoxin biosynthesis gene clusters and flanking regions. J Appl Microbiol 99 : 518 527.
10. 1. Int J Mol Sci 9 : 1717 1729.
11. Frisvad JC, Larson TO, de Vries R et al 2007) Secondary metabolite profiling, growth profiles and other tools for species recognition and important Aspergillus mycotoxins. Stud Mycol 59 : 31 37.
12. Holmes RA (2008) Characterization of an Aflatoxin Biosynthetic Gene and Resistance in Maize Seeds to Aspergillus flavus. PhD Thesis, North Carolina State University, Raleigh, NC.
13. Hsia MT Chu FS (1977) Reduction of aflatoxin B1 with zinc borohydride: an efficient preparation of aflatoxicol. Experientia 33 : 1132 1133.
14. Lau HP Chu FS (1983) Preparation and characterization of acid dehydration products of aflatoxicol. J Assoc Off Ana Chem 66 : 98 101.
15. Nakazato M, Morozumi S, Saito K, Fujinuma K, Nishima T Kasai N (1990) Interconversion of aflatoxin B1 and aflatoxicol by several fungi. Appl Environ Microb 56 : 1465 1470.
16. Prieto R, Yousibova GL Woloshuk CP (1996) Identification of aflatoxin biosynthesis genes by genetic complementation in an Aspergillus flavus mutant lacking the aflatoxin gene cluster. Appl Environ Microb 62 : 3567 3571.
17. Simpson TJ, Dejesus AE, Steyn PS Vleggaar R (1983) Biosynthesis of Aflatoxins - incorporation of [2-H-2(3)] acetate into aflatoxin-B1 by Aspergillus flavus. J Chem Soc, Chem Commun 1983 : 338 340, DOI: 10.1039/C39830000338.
18. Singh R Hsieh DP (1976) Enzymatic conversion of sterigmatocystin into aflatoxin B1 by cell-free extracts of Aspergillus parasiticus. Appl Environ Microb 31 : 743 745.
19. Udwary DW, Casillas LK Townsend CA (2002) Synthesis of 11-hydroxy-O-methylsterigmatocystin and the role of a cytochrome P-450 in the final step of aflatoxin biosynthesis. J Am Chem Soc 124 : 5294 5303.
20. Watanabe CM Townsend CA (1996) Incorporation of molecular oxygen in aflatoxin B1 biosynthesis. J Org Chem 61 : 1990 1993.
21. 2. Appl Environ Microb 64 : 4834 4841.
22. Yu J, Chang P-K, Ehrlich KC et al 2004) Clustered pathway genes in aflatoxin biosynthesis. Appl Environ Microb 70 : 1253 1262.