Molecular characterization of an Aspergillus parasiticus dehydrogenase gene, norA, located on the aflatoxin biosynthesis gene cluster

Applied and Environmental Microbiology

Volumn 62, Issue 2, 1996, Pages 360-366

  Cary, Jeffrey W ^a   Wright, Maureen ^a   Bhatnagar, Deepak ^a   Lee, Rachel ^b   Chu, Fun Sun ^b  

^a SOUTHERN REGIONAL RESEARCH CENTER   (United States)

^b UNIVERSITY OF WISCONSIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AFLATOXIN; ARYL ALCOHOL DEHYDROGENASE; COMPLEMENTARY DNA; FUNGAL DNA; FUNGAL RNA; NORSOLORINIC ACID DEHYDROGENASE; OXIDOREDUCTASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS FLAVUS; ASPERGILLUS PARASITICUS; BIOSYNTHESIS; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME ANALYSIS; GENE CLUSTER; NONHUMAN; PHANEROCHAETE;

EID: 0030068488     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.2.360-366.1996     Document Type: Article

References (34)

1. Adye, J., and R. I. Mateles. 1964. Incorporation of labelled compounds into aflatoxins. Biochim. Biophys. Acta 86:418-420.
2. Bhatnagar, D., et al. Unpublished data.
3. Bhatnagar, D., K. C. Ehrlich, and T. E. Cleveland. 1992. Oxidation-reduction reactions in biosynthesis of secondary metabolites and their control by environmental factors, p. 255-286. In D. Bhatnagar, E. B Lillehoj, and D. K. Arora (ed.), Handbook of applied mycology, vol. V. Marcel Dekker, Inc., New York.
4. Bhatnagar, D., J. E. Linz, G. A. Payne, and T. E. Cleveland. 1995. Molecular biology to eliminate aflatoxins. INFORM 6:262-271
5. Chang, P.-K., D. Bhatnagar, T. E. Cleveland, and J. Bennett. 1995. Sequence variability in homologs of the aflatoxin pathway gene aflR distinguishes species in Aspergillus section flavi. Appl. Environ. Microbiol. 61:40-43.
6. Chang, P.-K., J. W. Cary, D. Bhatnagar, T. E. Cleveland, J. W. Bennett, J. E. Linz, C. P. Woloshuk, and G. A. Payne. 1993. Cloning of the Aspergillus parasiticus apa-2 gene associated with the regulation of aflatoxin biosynthesis. Appl. Environ. Microbiol. 59:3273-3279
7. Chang, P.-K., J. W. Cary, J. Yu, D. Bhatnagar, and T. E. Cleveland. 1995. Aspergillus parasiticus polyketide synthase gene, pksA. is required for aflatoxin B1 biosynthesis Mol. Gen. Genet 248:270-277.
8. Chang, P.-K., K. C. Ehrlich, J. Yu, D. Bhatnagar, and T. E. Cleveland. 1994. Increased expression of aflR, encoding a sequence-specific DNA-binding protein, relieves nitrate inhibition of aflatoxin biosynthesis. Appl. Environ. Microbiol. 61:2372-2377
9. Chang, P.-K., C. D. Skory, and J. E. Linz. 1991. Cloning of a gene associated with aflatoxin B1 biosynthesis in Aspergillus parasiticus. Curr. Genet 20:231-233.
10. Chu, F. S. 1991 Mycotoxins: food contamination, mechanisms, carcinogenic potential and preventative measures. Mutat. Res. 259:291-306.
11. Chu, F. S. Unpublished data.
12. Chuturgoon, A. A., M. F. Dutton, and R. K. Berry. 1990. The preparation of an enzyme associated with aflatoxin biosynthesis by affinity chromatography Biochem. Biophys Res. Commun. 166:38-42.
13. Cleveland, T. E., A. R. Lax, L. S. Lee, and D. Bhatnagar. 1987. Appearance of enzyme activities catalyzing conversion of sterigmatocystin to aflatoxin B1 in late-growth-phase Aspergillus parasiticus cultures. Appl. Environ. Microbiol. 53:1711-1713.
14. Cole, R. J., and P. J. Cotty. 1990. Biocontrol of aflatoxin production by using biocompetitive agents, p. 62-68. In J. F. Robens (ed), A perspective on aflatoxin in field crops and animal food products in the United States. Agricultural Research Service, Beltsville, Md.
15. Cotty, P. J. 1994 Influence of field application of an atoxigenic strain of Aspergillus flavus on the populations of A flavus infecting cotton bolls and on the aflatoxin content of cottonseed Phytopathology 84:1270-1277
16. Detroy, R., S. Freer, and A. Ciegler. 1973 Aflatoxin and anthraquinone biosynthesis by nitrosoguanidine-derived mutants of Aspergillus parasiticus. Can J. Microbiol. 19:1373-1378.
17. Feng, G. H., F. S. Chu, and T. J. Leonard. 1992 Molecular cloning of genes related to aflatoxin biosynthesis by differential screening Appl Environ. Microbiol. 58:445-460
18. Keller, N. P., N. J. Kantz, and T. H. Adams. 1994. Aspergillus nidulans verA is required for production of the mycotoxin sterigmatocystin Appl Environ. Microbiol 60:1444-1450
19. Klich, M. A., et al. Unpublished observations
20. Lee, R. C., J. W. Cary, D. Bhatnagar, and F.-S. Chu. Production and characterization of polyclonal antibodies against norsolorimic acid reductase involved in aflatoxin biosynthesis. Food Agric. Immunol., in press.
21. Maramatsu, M. 1973 Preparation of RNA from animal cells. Methods Cell Biol. 7:23-51.
22. Muheim, A., R. Waldner, D. Sanglard, J. Reiser, H. E. Schoemaker, and M. S. A. Leisola. 1991. Purification and properties of and aryl-alcohol dehydrogenase from the white-rot fungus, Phanerochaete chrysosporium. Eur. J. Biochem 195:369-375.
23. Payne, G. A., G. J. Nystrom, D. Bhatnagar, T. E. Cleveland, and C. P. Wolashuk. 1993. Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus Appl. Environ. Microbiol. 59:156-162.
24. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad Sci. USA 74:5463-5467.
25. Scrutton, N. S., A. Berry, and R. N. Perham. 1990. Redesign of the coenzyme specificity of a dehydrogenase by protein engineering. Nature (London) 343:38-43.
26. Skory, C. D., P.-K. Chang, J. Cary, and J. E. Linz. 1992. Isolation and characterization of a gene from Aspergillus parasiticus associated with the conversion of versicolorin A to sterigmatocystin in aflatoxin biosynthesis, Appl. Environ. Microbiol. 58:3527-3537.
27. Stephen, D., C. Jones, and J. P. Schofield. 1990. A rapid method for isolating high quality plasmid DNA suitable for DNA sequencing. Nucleic Acids Res. 18:7463-7464.
28. Trail, F., P.-K. Chang, J. Cary, and J. E. Linz. 1994. Structural and functional analysis of the nor-1 gene involved in the biosynthesis of aflatoxins by Aspergillus parasiticus. Appl. Environ. Microbiol. 60:4078-4085.
29. Trail, F., N. Mahanti, and J. Linz. 1995. Molecular biology of aflatoxin biosynthesis Microbiology 141:755-765.
30. Woloshuk, C. P., and G. A. Payne. 1994 The alcohol dehydrogenase gene adhI is induced in Aspergillus flavus grown on medium conducive to aflatoxin biosynthesis. Appl. Environ. Microbiol. 60:670-676.
31. Yabe, K., Y. Nakamura, H. Nakajima, Y. Ando, and T. Hamasaki. 1991 Enzymatic conversion of norsolorinic acid to averufin in aflatoxin biosynthesis. Appl. Environ. Microbiol 57:1340-1345.
32. Yu, J., J. W. Cary, D. Bhatnagar, T. E. Cleveland, N. P. Keller, and F. S. Chu, 1993. Cloning and characterization of a cDNA from Aspergillus parasiticus encoding an O-methyltransferase involved in aflatoxin biosynthesis. Appl Environ. Microbiol. 59:3564-3571.
33. Yu, J., P.-K. Chang, J. W. Cary, M. Wright, D. Bhatnagar, T. E. Cleveland, G. A. Payne, and J. E. Linz. 1995. Comparative mapping of aflatoxin pathway gene clusters in Aspergillus parasiticus and Aspergillus flavus. Appl. Environ Microbiol 61:2365-2371.
34. Yu, J., P.-K. Chang, G. A. Payne, J. W. Cary, D. Bhatnagar, and T. E. Cleveland. 1995. Comparison of omtA gene encoding and O-methyltransferase involved in aflatoxin biosynthesis from Aspergillus parasiticus and Aspergillus flavus Gene 163:21-32.