Improved strains and plasmid vectors for conditional overexpression of His-tagged proteins in Haloferax volcanii

Applied and Environmental Microbiology

Volumn 76, Issue 6, 2010, Pages 1759-1769

  Allers, Thorsten ^a   Barak, Shahar ^b   Liddell, Susan ^a   Wardell, Kayleigh ^a   Mevarech, Moshe ^b  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b TEL AVIV UNIVERSITY   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

ARCHAEA; ARCHAEAL; HALOFERAX VOLCANII; HIS-TAGGED PROTEINS; HOST STRAIN; LINKER REGION; METAL-AFFINITY CHROMATOGRAPHY; ORTHOLOGS; OVER-EXPRESSION; PHARAONIS; PLASMID VECTORS; PROTEIN PURIFICATION; RECOMBINANT PROTEIN; RESTRICTION ENDONUCLEASES;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; BIOCHEMISTRY; CHROMOSOMES; ESCHERICHIA COLI; METAL REFINING; MICROBIOLOGY; PHOSPHATASES; PURIFICATION;

PROTEINS;

ARCHAEAL DNA; HYBRID PROTEIN; RESTRICTION ENDONUCLEASE;

CHROMATOGRAPHY; DNA; GENE EXPRESSION; MUTATION; PLASMID; PROKARYOTE; PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; DNA SEQUENCE; GENE EXPRESSION; GENE VECTOR; GENETIC ENGINEERING; GENETICS; HALOFERAX VOLCANII; METHODOLOGY; MICROBIAL GENETICS; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID;

DNA RESTRICTION-MODIFICATION ENZYMES; DNA, ARCHAEAL; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC VECTORS; GENETICS, MICROBIAL; HALOFERAX VOLCANII; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA;

ARCHAEA; ESCHERICHIA COLI; HALOFERAX VOLCANII; NATRONOMONAS PHARAONIS;

EID: 77749304005     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02670-09     Document Type: Article

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