An efficient process for the purification of helper-dependent adenoviral vector and removal of helper virus by iodixanol ultracentrifugation

Journal of Virological Methods

Volumn 165, Issue 1, 2010, Pages 83-89

  Dormond, Edwige ^a,b   Chahal, Parminder ^a   Bernier, Alice ^a   Tran, Rosa ^a   Perrier, Michel ^b   Kamen, Amine ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b ÉCOLE POLYTECHNIQUE DE MONTRÉAL   (Canada)

Author keywords

Chromatography; Helper dependent adenoviral vector; Iodixanol; Purification; Ultracentrifugation

Indexed keywords

ADENOVIRUS VECTOR; GREEN FLUORESCENT PROTEIN; HELPER DEPENDENT ADENOVIRUS VECTOR; IODIXANOL; LUCIFERASE; UNCLASSIFIED DRUG;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; DOWNSTREAM PROCESSING; GEL PERMEATION CHROMATOGRAPHY; GENE EXPRESSION; HELPER VIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; RELIABILITY; REPRODUCIBILITY; SEPARATION TECHNIQUE; VIRAL GENE THERAPY; VIRUS CONCENTRATION; VIRUS GENOME; VIRUS PURIFICATION;

ADENOVIRIDAE; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; GENETIC VECTORS; HELPER VIRUSES; HUMANS; TRIIODOBENZOIC ACIDS; ULTRACENTRIFUGATION; VIROLOGY;

EID: 77649338932     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.01.008     Document Type: Article

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