Purification and characterization of retrovirus vector particles by rate zonal ultracentrifugation

Journal of Virological Methods

Volumn 133, Issue 1, 2006, Pages 82-91

  Segura, María De Las Mercedes ^a,b   Garnier, Alain ^b   Kamen, Amine ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b UNIVERSITÉ LAVAL   (Canada)

Author keywords

Gene therapy; Iodixanol; Purification; Retrovirus; Ultracentrifugation

Indexed keywords

IODIXANOL; SUBTILISIN;

ARTICLE; CELL SUSPENSION; ELECTRON MICROSCOPY; GEL PERMEATION CHROMATOGRAPHY; HUMAN; HUMAN CELL; MEMBRANE VESICLE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; RETROVIRUS VECTOR; SEPARATION TECHNIQUE; STAINING; SUPERNATANT; ULTRACENTRIFUGATION; VIRUS CHARACTERIZATION; VIRUS PARTICLE; VIRUS PURIFICATION; VIRUS RECOMBINANT;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; CHROMATOGRAPHY, GEL; DNA, VIRAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDONUCLEASES; FILTRATION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; MICE; MOLONEY MURINE LEUKEMIA VIRUS; PARTICLE SIZE; RECOMBINANT FUSION PROTEINS; RETROVIRIDAE; SILVER STAINING; SIMPLEXVIRUS; SUBTILISIN; THYMIDINE KINASE; TRIIODOBENZOIC ACIDS; ULTRACENTRIFUGATION; VIRION;

EID: 33644919393     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2005.10.030     Document Type: Article

References (26)

1. A. Abe, A. Miyanohara, and T. Friedmann Enhanced gene transfer with fusogenic liposomes containing vesicular stomatitis virus G glycoprotein J. Virol. 72 1998 6159 6163
2. R. Alain Quantitation of virus particles by negative stain electron microscopy Microsc. Today 97 1997 20
3. R. Alain, F. Nadon, C. Seguin, P. Payment, and M. Trudel Rapid virus subunit visualization by direct sedimentation of samples on electron microscope grids J. Virol. Meth. 16 1987 209 216
4. J.W. Bess Jr., R.J. Gorelick, W.J. Bosche, L.E. Henderson, and L.O. Arthur Microvesicles are a source of contaminating cellular proteins found in purified HIV-1 preparations Virology 230 1997 134 144
5. M. Dettenhofer, and X.F. Yu Highly purified human immunodeficiency virus type 1 reveals a virtual absence of Vif in virions J. Virol. 73 1999 1460 1467
6. M.T. Esser, D.R. Graham, L.V. Coren, C.M. Trubey, J.W. Bess Jr., L.O. Arthur, D.E. Ott, and J.D. Lifson Differential incorporation of CD45, CD80 (B7-1), CD86 (B7-2), and major histocompatibility complex class I and II molecules into human immunodeficiency virus type 1 virions and microvesicles: implications for viral pathogenesis and immune regulation J. Virol. 75 2001 6173 6182
7. P. Gluschankof, I. Mondor, H.R. Gelderblom, and Q.J. Sattentau Cell membrane vesicles are a major contaminant of gradient-enriched human immunodeficiency virus type-1 preparations Virology 230 1997 125 133
8. M. Hammarstedt, K. Wallengren, K.W. Pedersen, N. Roos, and H. Garoff Minimal exclusion of plasma membrane proteins during retroviral envelope formation Proc. Natl. Acad. Sci. U.S.A. 97 2000 7527 7532
9. M. Kuiper, R.M. Sanches, J.A. Walford, and N.K. Slater Purification of a functional gene therapy vector derived from Moloney murine leukaemia virus using membrane filtration and ceramic hydroxyapatite chromatography Biotechnol. Bioeng. 80 2002 445 453
10. C.A. Laughlin, M.W. Myers, D.L. Risin, and B.J. Carter Defective-interfering particles of the human parvovirus adeno-associated virus Virology 94 1979 162 174
11. A. Moller-Larsen, and T. Christensen Isolation of a retrovirus from multiple sclerosis patients in self-generated Iodixanol gradients J. Virol. Meth. 73 1998 151 161
12. D.S. Ory, B.A. Neugeboren, and R.C. Mulligan A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes Proc. Natl. Acad. Sci. U.S.A. 93 1996 11400 11406
13. D.E. Ott Cellular proteins in HIV virions Rev. Med. Virol. 7 1997 167 180
14. D.E. Ott Potential roles of cellular proteins in HIV-1 Rev. Med. Virol. 12 2002 359 374
15. D.E. Ott, L.V. Coren, D.G. Johnson, R.C. Sowder 2nd, L.O. Arthur, and L.E. Henderson Analysis and localization of cyclophilin A found in the virions of human immunodeficiency virus type 1 MN strain AIDS Res. Hum. Retroviruses 11 1995 1003 1006
16. D.E. Ott, L.V. Coren, B.P. Kane, L.K. Busch, D.G. Johnson, R.C. Sowder 2nd, E.N. Chertova, L.O. Arthur, and L.E. Henderson Cytoskeletal proteins inside human immunodeficiency virus type 1 virions J. Virol. 70 1996 7734 7743
17. A. Paquin, D.E. Jaalouk, and J. Galipeau Retrovector encoding a green fluorescent protein-herpes simplex virus thymidine kinase fusion protein serves as a versatile suicide/reporter for cell and gene therapy applications Hum. Gene. Ther. 12 2001 13 23
18. F. Ruchti, G. Siegl, and M. Weitz Identification and characterization of incomplete hepatitis A virus particles J. Gen. Virol. 72 Pt 9 1991 2159 2166
19. M. Segura, A. Kamen, P. Trudel, and A. Garnier A novel purification strategy for retrovirus gene therapy vectors using heparin affinity chromatography Biotechnol. Bioeng. 90 2005 391 404
20. M. Toth, B. Tarodi, and I. Beladi Preparative separation of intact incomplete and empty adenovirus type 2 particles Acta Virol. 26 1982 217 220
21. J. Transfiguracion, D.E. Jaalouk, K. Ghani, J. Galipeau, and A. Kamen Size-exclusion chromatography purification of high-titer vesicular stomatitis virus G glycoprotein-pseudotyped retrovectors for cell and gene therapy applications Hum. Gene. Ther. 14 2003 1139 1153
22. C.M. Trubey, E. Chertova, L.V. Coren, J.M. Hilburn, C.V. Hixson, K. Nagashima, J.D. Lifson, and D.E. Ott Quantitation of HLA class II protein incorporated into human immunodeficiency type 1 virions purified by anti-CD45 immunoaffinity depletion of microvesicles J. Virol. 77 2003 12699 12709
23. G. Vellekamp, F.W. Porter, S. Sutjipto, C. Cutler, L. Bondoc, Y.H. Liu, D. Wylie, S. Cannon-Carlson, J.T. Tang, A. Frei, M. Voloch, and S. Zhuang Empty capsids in column-purified recombinant adenovirus preparations Hum. Gene. Ther. 12 2001 1923 1936
24. S.L. Williams, M.E. Eccleston, and N.K. Slater Affinity capture of a biotinylated retrovirus on macroporous monolithic adsorbents: towards a rapid single-step purification process Biotechnol. Bioeng. 89 2005 783 787
25. K. Ye, S. Jin, M.M. Ataai, J.S. Schultz, and J. Ibeh Tagging retrovirus vectors with a metal binding peptide and one-step purification by immobilized metal affinity chromatography J. Virol. 78 2004 9820 9827
26. L. Zhou, Q. Yu, and M. Luo Characterization of two density populations of feline calicivirus particles Virology 205 1994 530 533