Structural characterization and cytolytic activity of a potent antimicrobial motif in longicin, a defensin-like peptide in the tick Haemaphysalis longicornis

Journal of Veterinary Medical Science

Volumn 72, Issue 2, 2010, Pages 149-156

  Rahman, Md Morshedur ^a   Tsuji, Naotoshi ^b   Boldbaatar, Damdinsuren ^a   Battur, Banzragch ^a   Liao, Min ^a   Umemiya Shirafuji, Rika ^a   You, Myungjo ^c   Tanaka, Tetsuya ^a   Fujisaki, Kozo ^a  

^a KAGOSHIMA UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF ANIMAL HEALTH   (Japan)

^c Chonbuk National University   (South Korea)

Author keywords

Antimicrobial peptides; Longicin; P4; Structure

Indexed keywords

ANTIMICROBIAL CATIONIC PEPTIDE; DEFENSIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CELL PROLIFERATION; CERCOPITHECUS; CHEMICAL STRUCTURE; CHEMISTRY; CIRCULAR DICHROISM; DRUG EFFECT; HEMOLYSIS; HYDROPHOBICITY; MOLECULAR GENETICS; PROTEIN MOTIF; PROTEIN SECONDARY STRUCTURE; TICK; VERO CELL;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; ANTIMICROBIAL CATIONIC PEPTIDES; CELL PROLIFERATION; CERCOPITHECUS AETHIOPS; CIRCULAR DICHROISM; DEFENSINS; HEMOLYSIS; HYDROPHOBICITY; IXODIDAE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, SECONDARY; VERO CELLS;

HAEMAPHYSALIS LONGICORNIS; IXODIDA; IXODIDAE;

EID: 77649332156     PISSN: 09167250     EISSN: 13477439     Source Type: Journal    
DOI: 10.1292/jvms.09-0167     Document Type: Article

References (40)

1. Ahmad, I., Perkins, W. R., Lupan, D. M., Selsted, M. E. and Janoff, S. A. 1995. Liposomal entrapment of the neutrophilderived peptide indolicidin endows it with in vivo antifungal activity. Biochim. Biophys. Acta 1237: 109-114.
2. Beier, J. C. 1998. Malaria parasite development in mosquitoes. Annu. Rev. Entomol. 43: 519-543.
3. Ceraul, S. M., Dreher-Lesnick, S. M., Gillespie, J. J., Rahman, M. S. and Azad, A. F. 2007. New tick defensin isoform and antimicrobial gene expression in response to Rickettsia monatanensis challenge. Infect. Immun. 75: 1973-1983.
4. Dathe, M., Nikolenko, H., Meyer, J., Beyermann, M. and Bienert, M. 2001. Optimization of the antimicrobial activity of magainin peptides by modification of charge. FEBS Lett. 501: 146-150.
5. Dimopoulos, G., Muller, H. M., Levashina, E. A. and Kafatos, F. C. 2001. Innate immune defense against malaria infection in the mosquito. Curr. Opin. Immunol. 13: 79-88.
6. Fogaca, A. C., da Sliva, P. I., Miranda, M. T., Bianchi, A. G., Miranda, A., Ribolla, P. E. and Daffre, S. 1999. Antimicrobial activity of a bovine hemoglobin fragment in the tick Boophilus microplus. J. Biol. Chem. 274: 25330-25334.
7. Fogaca, A. C., Lorenzini, D. M., Kaku, L. M., Esteves, E., Bulet, P. and Daffre, S. 2004. Cysteine-rich antimicrobial peptides of the cattle tick Boophilus microplus: isolation, structural characterization and tissue expression profile. Dev. Comp. Immunol. 28: 191-200.
8. Gura, T. 2001. Innate immunity. Ancient system gets new respect. Science 291: 2068-2071.
9. Hancock, R. E. W. and Patrzykat, A. 2002 Clinical development of cationic antimicrobial peptides: from natural to novel antibiotics. Curr. Drug Targets Infect. Disord. 2: 79-83.
10. Hao, G., Shi, Y. H., Han, J. H., Li, Q. H., Tang, Y. L. and Le, G. W. 2007. Design and analysis of structure-activity relationship of novel antimicrobial peptides derived from the conserved sequence of cecropin. J. Peptide Sci. 14: 290-298.
11. Hoffmann, J. A. 1997. Immune responsiveness in vector insects. Proc. Natl. Acad. Sci. U.S.A. 94: 11152-11153.
12. Hwang, P. M. and Vogel, H. J. 1998. Structure function relationships of antimicrobial peptides. Biochem. Cell Biol. 76: 235-246.
13. Hynes, W. L., Ceraul, S. M., Todd, S. M., Seguin, K. C. and Sonenshine, D. E. 2005. A defensin-like gene expressed in the black-legged tick, Ixodes scapularis. Med. Vet. Entomol. 19: 339-344.
14. Javadpour, M. M. and Barkley, M. D. 1997. Self-assembly of designed antimicrobial activity peptides in solution and micelles. Biochemistry 36: 9540-9549.
15. Jenssen, H., Hamill, P. and Hancock, R. E. W. 2006. Peptide antimicrobial agents. Clin. Microbiol. Rev. 19: 491-511.
16. Johanson, J., Gudmundsson, G. H., Rottenberg, M., Berndt, K. D. and Agerbeth, B. 1998. Conformation-dependent antibacterial activity of the naturally occurring human peptide LL-37. J. Biol. Chem. 273: 3718-3724.
17. Johns, R., Sonenshie, D. E. and Hynes, W. L. 2001. Identification of a defensin from the hemolymph of the American dog tick Dermacentor variabilis. Insect Biochem. Mol. Biol. 31: 857-865.
18. Kamysz, W., Okroj, M. and Lukasiak, J. 2003. Novel properties of antimicrobial peptides. Acta Biochim. Polo. 5: 461-469.
19. Lai, R., Lomas, L. O., Jonczy, J., Turner, P. C. and Rees, H. H. 2004. Two novel non-cationic defensin-like antimicrobial peptides from haemolymph of the female tick, Amblyomma hebraeum. Biochem. J. 379: 681-685.
20. Lee, D. G., Kim, H. N., Park, Y., Kim, H. K., Choi, C. H. and Hahm, K. S. 2002. Design of novel analogue peptides with potent antibiotic activity based on the antimicrobial peptide, HP (2-20) derived from N-terminus of Helicobacter pylori ribosomal protein L1. Biochim. Biophys. Acta 1598: 185-194.
21. Lehrer, R. I. and Ganz, T. 1999. Antimicrobial peptides in mammalian and insect host defence. Curr. Opin. Immunol. 11: 23-27.
22. Matsuzaki, K., Yoneyama, S., Fujii, N., Miyajima, K., Yamada, K., Kirino, Y. and Anzali, K. 1997. Membrane permeabilization mechanisms of cyclic antimicrobial peptide, tachyplesin1 and its linear analog. Biochemistry 36: 9799-9806.
23. Nakajima, Y., van der Goes van Naters-Yasui, A., Taylor, D. and Yamakawa, M. 2002. Antibacterial peptide defensin is involved in midgut immunity of the soft tick, Ornithodoros moubata. Insect Mol. Biol. 11: 611-618.
24. Pathak, N., Salas-Auvert, R., Ruche, G., Janna, M. H., McCarthy, D. and Harrison, R. G. 1995. Comparison of the effects of hydrophobicity, amphipathicity, and alpha-helicity on the activities of antimicrobial peptides. Proteins 22: 182-186.
25. Potterton, L., McNicholas, S., Krissinel, E., Gruber, J., Cowtan, K., Emsley, P., Murshudov, G. N., Cohen, S., Perrakis, A. and Noble, M. 2004. Developments in the CCP4 molecular-graphics project. Acta Crystallogr. D. 60: 2288-2294.
26. Powers, J. P. and Hancock, R. E. W. 2003. The relationship between peptide structure and antibacterial activity. Peptides 24: 1681-1691.
27. Rudenko, N., Golovchenko, M., Edwards, M. J. and Grubhoffer, L. 2005. Differential expression of Ixodes ricinus tick genes induced by blood feeding or Borrelia burgdorferi infection. J. Med. Entomol. 42: 36-41.
28. Saberwal, G. and Nagaraj, R. 1994. Cell-lytic and antibacterial peptides that act by perturbing the barrier function of membranes: facets of their conformational features, structure-function co-relations, and membrane perturbing abilities. Biochem. Biophys. Acta 1197: 109-131.
29. Skerlavaj, B., Benincasa, M., Risso, A., Zanetti, M. and Gennaro, R. 1999. SMAP-29, a potent antibacterial and antifungal peptide from sheep leukocytes. FEBS Lett. 463: 58-62.
30. Skerlavaj, B., Gennaro, R., Bagella, L., Merluzzi, L., Risso, A. and Zanetti, M. 1996. Biological characterization of two novel cathelicidin-derived peptides and identification of structural requirements for their antimicrobial and cell lytic activities. J. Biol. Chem. 271: 28375-28381.
31. Stark, M., Liu, L. P. and Deber, C. M. 2002. Cationic hydrophobic peptides with antimicrobial activity. Antimirob. Agents Chemother. 46: 3585-3590.
32. Subbalakshmi, C., Nagaraj, R. and Sitaram, N. 1999. Biological activities of C-terminal 15-residue synthetic fragment of melittin: design of an analog with improved antibacterial activity. FEBS Lett. 448: 62-66.
33. Tsuji, N., Battsetseg, B., Boldbaatar, D., Miyoshi, T., Xuan, X., Oliver, J. H. and Fujisaki, K. 2007. Babesial vector tick defensin against Babesia sp. parasites. Infect. Immun. 75: 3633-3640.
34. Yang, Y. S., Mitta, G., Chavanieu, A., Calas, B., Sanchez, J. F., Roch, P. and Aumelas, A. 2000. Solution structure and activity of the synthetic four-disulfide bond Mediterranean mussel defensin (MGD-1). Biochemistry 39: 14436-14447.
35. Yang, S. T., Shin, S. Y., Hahmb, K. S. and Kim, J. I. 2006. Design of perfectly symmetric Trp-rich peptides with potent and broad-spectrum antimicrobial activities. Int. J. Antimicrob. Agents 27: 325-330.
36. Yeaman, M. R. and Yount, N. Y. 2003. Mechanism of antimicrobial peptide action and resistance. Pharmacol. Rev. 55: 27-55.
37. Zanetti, M., Gennaro, R., Skerlavaj, B., Tomasinsig, L. and Circo, R. 2002. Cathelicidin peptides as candidates for a novel class of antimicrobials. Curr. Pharm. Dis. 8: 779-793.
38. Zasloff, M. 2002. Antimicrobial peptides of multicellular organisms. Nature 415: 389-395.
39. Zhai, Y. and Saier, M. H. Jr. 2001. A web-based program (WHAT) for the simultaneous prediction of hydropathy, amphipathicity, secondary structure and transmembrane topology for a single protein sequence. J. Mol. Microbiol. Biotechnol. 3: 501-502.
40. Zhou, J., Liao, M., Ueda, M., Gong, H., Xuan, X. and Fujisaki, K. 2007. Sequence characterization and expression patterns of two defensin-like antimicrobial peptides from the tick Haemaphysalis longicornis. Peptides 28: 1304-1310.