The Structure of the Salmonella typhimurium Type III Secretion System Needle Shows Divergence from the Flagellar System

Journal of Molecular Biology

Volumn 396, Issue 5, 2010, Pages 1392-1397

  Galkin, Vitold E ^a   Schmied, Wolfgang H ^b,c   Schraidt, Oliver ^b,c   Marlovits, Thomas C ^b,c   Egelman, Edward H ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

^b RESEARCH INSTITUTE OF MOLECULAR PATHOLOGY   (Austria)

^c ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

Author keywords

Bacterial pathogenesis; Cryo electron microscopy; Helical reconstruction; Protein polymers; Structural polymorphism

Indexed keywords

ARTICLE; BACTERIAL FLAGELLUM; BACTERIAL VIRULENCE; CONTROLLED STUDY; HOST CELL; HOST PATHOGEN INTERACTION; NONHUMAN; PRIORITY JOURNAL; SALMONELLA TYPHIMURIUM; SHIGELLA; TYPE III SECRETION SYSTEM;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; SALMONELLA TYPHIMURIUM; SHIGELLA; SHIGELLA FLEXNERI;

EID: 77649275030     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2010.01.001     Document Type: Article

References (20)

1. Galan J.E. Salmonella interactions with host cells: type III secretion at work. Annu. Rev. Cell Dev. Biol. 2001, 17:53-86.
2. Desvaux M., Hebraud M., Henderson I.R., Pallen M.J. Type III secretion: what's in a name?. Trends Microbiol. 2006, 14:157-160.
3. Blocker A., Komoriya K., Aizawa S. Type III secretion systems and bacterial flagella: insights into their function from structural similarities 1. Proc. Natl Acad. Sci. USA 2003, 100:3027-3030.
4. Cordes F.S., Komoriya K., Larquet E., Yang S., Egelman E.H., Blocker A., Lea S.M. Helical structure of the needle of the type III secretion system of Shigella flexneri. J. Biol. Chem. 2003, 278:17103-17107.
5. Yonekura K., Maki-Yonekura S., Namba K. Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy. Nature 2003, 424:643-650.
6. Cordes F.S., Daniell S., Kenjale R., Saurya S., Picking W.L., Picking W.D., et al. Helical packing of needles from functionally altered Shigella type III secretion systems. J. Mol. Biol. 2005, 354:206-211.
7. Yamashita I., Hasegawa K., Suzuki H., Vonderviszt F., Mimori-Kiyosue Y., Namba K. Structure and switching of bacterial flagellar filaments studied by X-ray fiber diffraction. Nat. Struct. Biol. 1998, 5:125-132.
8. Egelman E.H., DeRosier D.J. The Fourier transform of actin and other helical systems with cumulative random angular disorder. Acta Crystallogr. Sect. A 1982, 38:796-799.
9. Egelman E.H. The iterative helical real space reconstruction method: surmounting the problems posed by real polymers. J. Struct. Biol. 2007, 157:83-94.
10. Egelman E.H. A robust algorithm for the reconstruction of helical filaments using single-particle methods. Ultramicroscopy 2000, 85:225-234.
11. Wall J.S., Hainfeld J.F. Mass mapping with the scanning transmission electron microscope. Annu. Rev. Biophys. Biophys. Chem. 1986, 15:355-376.
12. Wang Y., Ouellette A.N., Egan C.W., Rathinavelan T., Im W., De Guzman R.N. Differences in the electrostatic surfaces of the type III secretion needle proteins PrgI, BsaL, and MxiH. J. Mol. Biol. 2007, 371:1304-1314.
13. Deane J.E., Roversi P., Cordes F.S., Johnson S., Kenjale R., Daniell S., et al. Molecular model of a type III secretion system needle: implications for host-cell sensing. Proc. Natl Acad Sci. USA 2006, 103:12529-12533.
14. Galkin V.E., Orlova A., Cherepanova O., Lebart M.C., Egelman E.H. High-resolution cryo-EM structure of the F-actin-fimbrin/plastin ABD2 complex. Proc. Natl Acad. Sci. USA 2008, 105:1494-1498.
15. Darboe N., Kenjale R., Picking W.L., Picking W.D., Middaugh C.R. Physical characterization of MxiH and PrgI, the needle component of the type III secretion apparatus from Shigella and Salmonella. Protein Sci. 2006, 15:543-552.
16. Fujii T., Kato T., Namba K. Specific arrangement of alpha-helical coiled-coils in the core domain of the bacterial flagellar hook for the universal joint function. Structure 2009, 17:1485-1493.
17. Galkin V.E., Yu X., Bielnicki J., Heuser J., Ewing C.P., Guerry P., Egelman E.H. Divergence of quaternary structures among bacterial flagellar filaments. Science 2008, 320:382-385.
18. Kenjale R., Wilson J., Zenk S.F., Saurya S., Picking W.L., Picking W.D., Blocker A. The needle component of the type III secreton of Shigella regulates the activity of the secretion apparatus. J. Biol. Chem. 2005, 280:42929-42937.
19. Kubori T., Sukhan A., Aizawa S.I., Galan J.E. Molecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion system. Proc. Natl Acad. Sci. USA 2000, 97:10225-10230.
20. Marlovits T.C., Kubori T., Sukhan A., Thomas D.R., Galán J.E., Unger V.M. Structural insights into the assembly of the type III secretion needle complex. Science 2004, 306:1040-1042.