Structural insights into the assembly of the type III secretion needle complex

Science

Volumn 306, Issue 5698, 2004, Pages 1040-1042

  Marlovits, Thomas C ^a   Kubori, Tomoko ^a   Sukhan, Anand ^a,c   Thomas, Dennis R ^b   Galán, Jorge E ^a   Unger, Vinzenz M ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b BRANDEIS UNIVERSITY   (United States)

^c OKLAHOMA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CONFORMATIONS; MOLECULAR STRUCTURE; OLIGOMERS; PROTEINS; VIRUSES;

SALMONELLA TYPHIMURIUM; VIRULENCE FACTORS;

BACTERIOLOGY;

BACTERIAL PROTEIN; OLIGOMER; PROTEIN PRECURSOR; PROTEIN PRGJ; UNCLASSIFIED DRUG;

BIOLOGY;

ARTICLE; BACTERIAL MEMBRANE; BACTERIAL TRANSLOCATION; BACTERIAL VIRULENCE; COMPLEX FORMATION; IN VIVO STUDY; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN SECRETION; PROTEIN TRANSPORT; SALMONELLA TYPHIMURIUM; STRUCTURE ANALYSIS; SURFACE PROPERTY; TYPE III SECRETION NEEDLE COMPLEX;

BACTERIAL PROTEINS; CRYOELECTRON MICROSCOPY; MACROMOLECULAR SUBSTANCES; MEMBRANE TRANSPORT PROTEINS; SALMONELLA TYPHIMURIUM;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; SALMONELLA TYPHIMURIUM;

EID: 8344258355     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1102610     Document Type: Article

References (16)

1. G. R. Cornelis, F. Van Gijsegem, Annu. Rev. Microbiol. 54, 735 (2000).
2. J. E. Galán, A. Collmer, Science 284, 1322 (1999).
3. T. Kubori et al., Science 280, 602 (1998).
4. T. Kubori, A. Sukhan, S. I. Aizawa, J. E. Galán, Proc. Natl. Acad. Sci. U.S.A. 97, 10225 (2000).
5. J. E. Galán, R. Curtiss III, Proc. Natl. Acad. Sci. U.S.A. 86, 6383 (1989).
6. Materials and methods are available as supporting material on Science Online.
7. T. C. Marlovits, T. Kubori, J. E. Galán, V. Unger, unpublished results.
8. M. Valle et al., EMBO J. 21, 3557 (2002).
9. S. Yang et al., J. Mol. Biol. 321, 839 (2002).
10. A. Sukhan, T. Kubori, J. E. Galán, J. Bacteriol. 185, 3480 (2003).
11. C. Collazo, J. E. Galán, Infect. Immun. 64, 3524 (1996).
12. A. Sukhan, T. Kubori, J. Wilson, J. E. Galán, J. Bacteriol. 183, 1159 (2001).
13. T. G. Kimbrough, S. I. Miller, Proc. Natl. Acad. Sci. U.S.A. 97, 11008 (2000).
14. J. E. Galán, Annu. Rev. Cell Dev. Biol. 17, 53 (2001).
15. A. Blocker et al., Mol. Microbiol. 39, 652 (2001).
16. We thank D. J. DeRosier and F. Sigworth for helpful discussions about the image processing. We are grateful to the Yale School of Medicine for the support of the Cryo Electron Microscopy Core Facility; N. Grigorieff (Brandeis University) for the use of a computer cluster; and the Howard Hughes Medical Institute Keck Facility at Yale for the quantitative amino acid analysis. Molecular graphics images (Fig. 2) were produced using the UCSF Chimera package from the Computer Graphics Laboratory, University of California, San Francisco (NIH P42 RR-01081). This work was supported by Public Health Service grants GM35433 from the NIH to D. J. DeRosier (supporting D.R.T), AI30492 to J.E.G, and GM66145 to V.M.U. and by a Hellman Family Fellowship to V.M.U. The structure of the Needle Complex has been deposited in the EmDep Database with accession code EMD-1100.