Replication of phenotypically mixed human immunodeficiency virus type 1 virions containing catalytically active and catalytically inactive reverse transcriptase

Journal of Virology

Volumn 75, Issue 14, 2001, Pages 6537-6546

  Julias, J G ^a   Ferris, A L ^a   Boyer, P L ^a   Hughes, S H ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD24 ANTIGEN; CELL SURFACE MARKER; RIBONUCLEASE; RNA DIRECTED DNA POLYMERASE;

ARTICLE; DNA SYNTHESIS; ENZYME ACTIVITY; GENE MUTATION; GENETIC TRANSFECTION; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION; VIRION; VIRUS REPLICATION;

CELLS, CULTURED; GENETIC VECTORS; HIV-1; HIV-1 REVERSE TRANSCRIPTASE; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; RIBONUCLEASE H, CALF THYMUS; RNA-DIRECTED DNA POLYMERASE; TRANSFECTION; VIRUS REPLICATION;

EID: 0034990535     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.75.14.6537-6546.2001     Document Type: Article

References (69)

1. Production of high-titer human immunodeficiency virus type 1 pseudotyped with vesicular stomatitis virus glycoprotein
2. Effects on DNA synthesis and translocation caused by mutations in the RNase H domain of Moloney murine leukemia virus reverse transcriptase
3. Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1
4. Mutational analysis of the fingers domain of human immunodeficiency virus type 1 reverse transcriptase
5. A mutation at position 190 of human immunodeficiency virus type 1 reverse transcriptase interacts with mutations at positions 74 and 75 via the template primer
6. Vpr is required for efficient replication of human immunodeficiency virus type-1 in mononuclear phagocytes
7. Crystal structure of the ribonuclease H domain of HIV-1 reverse transcriptase
8. Polymerization and RNase H activities of the reverse transcriptases from avian myeloblastosis, human immunodeficiency and Moloney murine leukemia viruses are functionally uncoupled
9. Characterization of highly immunogenic p66/p55 as the reverse transcriptase of HTL VIII/LAV
10. In vitro analysis of human immunodeficiency virus type 1 minus-strand strong-stop DNA synthesis and genomic RNA processing
11. Analysis of the RNA- and DNA-dependent DNA polymerase activities of point mutants of HIV-1 reverse transcriptase lacking ribonuclease H activity
12. Rapid kinetic analysis of a point mutant of HIV-1 reverse transcriptase lacking ribonuclease H activity
13. When retroviral reverse transcriptases reach the ends of their RNA templates
14. Human immunodeficiency virus reverse transcriptase ribonuclease H: Specificity of tRNA(Lys3)-primer excision
15. Reverse-transcriptase-RNaseH from human immunodeficiency virus: Relationship of the DNA polymerase and RNA hydrolysis activities
16. Effects of mutations in the polymerase domain on the polymerase, RNase H and strand transfer activities of human immunodeficiency virus type 1 reverse transcriptase
17. A detailed model of reverse transcription and tests of crucial aspects
18. Human immunodeficiency virus type 1 reverse transcriptase: Spatial and temporal relationship between the polymerase and RNase H activities
19. Defects in primer-template binding, processive DNA synthesis, and RNase H activity associated with the chimeric reverse transcriptases having the murine leukemia virus polymerase domain joined to Escherichia coli RNase H
20. Viral polyproteins in chick embryo fibroblasts infected with avian sarcoma leukosis viruses
21. cdc2 activity
22. Real time quantitative PCR
23. Reconstitution in vitro of RNase H activity by using purified N-terminal and C-terminal domains of HIV-1 reverse transcriptase
24. RNase H of retroviral reverse transcriptases
25. lys
26. Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 Å resolution shows bent DNA
27. Further characterization of intracellular precursor polyproteins of Rauscher leukemia virus
28. Three-dimensional structure of ribonuclease H from E. coli
29. 2+ dependent RNase H activity
30. Crystal structure at 3.5 Å resolution of HIV-1 reverse transcriptase complexed with an inhibitor
31. Isolation of cloned Moloney murine leukemia virus reverse transcriptase lacking ribonuclease H activity
32. Subunit selective mutagenesis indicates minimal polymerase activity in heterodimer-associated p51 HIV-1 reverse transcriptase
33. Functional organization of the murine leukemia virus reverse transcriptase: Characterization of a bacterially expressed AKR DNA polymerase deficient in RNase H activity
34. Advances in quantitative PCR technology; 5′ nuclease assays
35. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization
36. Template switching by reverse transcriptase during DNA synthesis
37. Synthesis of a 600-nucleotide-long plus-strand DNA by virions Moloney murine leukemia virus
38. Site-directed mutagenesis of the conserved Asp-443 and Asp-498 carboxy-terminal residues of HIV-1 reverse transcriptase
39. Mutagenesis of the conserved aspartic acid 443, glutamic acid 478, aspargine 494, and aspartic acid 498 residues in the ribonuclease H domain of p66/p51 human immunodeficiency virus type 1 reverse transcriptase
40. A joint product of the genes gag and pol of avian sarcoma virus: A possible precursor of reverse transcriptase
41. Mutations within the primer grip region of reverse transcriptase result in loss of RNase H function
42. A large deletion in the connection subdomain of murine leukemia virus reverse transcriptase or replacement of the RNase H domain with Escherichia coil RNase H results in altered polymerase and RNase H activities
43. Plus-strand origin for human immunodeficiency virus type 1: Implications for integration
44. Incomplete removal of the RNA primer for minus-strand DNA synthesis by human immunodeficiency virus type 1 reverse transcriptase
45. Plus-strand priming by Moloney murine leukemia virus. The sequence features important for cleavage by RNase H
46. Inhibition of RNase H activity and viral replication by single mutations in the 3′ region of moloney murine leukemia virus reverse transcriptase
47. Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA
48. Inactivation of the RNase H domain of HIV-1 reverse transcriptase blocks viral infectivity
49. The catalytic functions of chimeric reverse transcriptases of human immunodeficiency viruses type 1 and type 2
50. Specificity of initiation of plus-strand DNA by Rous sarcoma virus
51. Lys
52. Contributions of DNA polymerase subdomains to the RNase H activity of human immunodeficiency virus type 1 reverse transcriptase
53. Construction of an enzymatically active ribonuclease H domain of human immunodeficiency virus type 1 reverse transcriptase
54. Structural studies of avian myeloblastosis virus: Comparison of polypeptides in virion and core component by dodecyl sulfate-polyacrylamide gel electrophoresis
55. Abortive reverse transcription by mutants of Moloney murine leukemia virus deficient in the reverse transcriptase-associated RNase H function
56. Domain structure of the Moloney murine leukemia virus reverse transcriptase: Mutational analysis and separate expression of the polymerase and RNase H activities
57. Reverse transcription of avian sarcoma virus RNA into DNA might involve copying of the tRNA primer
58. Defects in Moloney murine leukemia virus replication caused by a reverse transcriptase mutation modeled on the structure of Escherichia coil ribonuclease H
59. Two defective forms of reverse transcriptase can complement to restore retroviral infectivity
60. Mutations within the RNase H domain of HIV-1 reverse transcriptase abolish viral infectivity
61. Kinetics, of synthesis, structure, and purification of avian sarcoma virus-specific DNA made in the cytoplasm of acutely infected cells
62. Enzymatic analysis of two HIV-1 reverse transcriptase mutants with mutations in the carboxyl-terminal amino acid residues conserved among retroviral ribonucleases H
63. The heat stable antigen (mouse CD24) gene is differentially regulated but has a housekeeping promoter
64. Sequence of the circle junction of human immunodeficiency virus type 1: Implications for reverse transcription and integration
65. Retroviral reverse transcription and integration: Progress and problems
66. Interaction of HIV-1 ribonuclease H with polypurine tract containing RNA-DNA hybrids
67. Structure of ribonuclease H phased at 2 Å resolution by MAD analysis of the selenomethionyl protein
68. Generation of high-titer pseudotyped retroviral vectors with very broad host range