Catalytic domain of PDC-E2 contains epitopes recognized by antimitochondrial antibodies in primary biliary cirrhosis

World Journal of Gastroenterology

Volumn 16, Issue 8, 2010, Pages 973-981

  Braun, Sandra ^a   Berg, Christoph ^a   Buck, Sandra ^a   Gregor, Michael ^a   Klein, Reinhild ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Active site; Anti M2; Catalytic domain; E2 subunit; Epitope mapping; Inner lipoyl domain; Primary biliary cirrhosis; Pyruvate dehydrogenase complex

Indexed keywords

DIHYDROLIPOAMIDE DEHYDROGENASE; EPITOPE; MITOCHONDRION ANTIBODY; OVALBUMIN; PYRUVATE DEHYDROGENASE COMPLEX; URSODEOXYCHOLIC ACID;

ADULT; AGED; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME SUBUNIT; FEMALE; HUMAN; LIVER BIOPSY; MAJOR CLINICAL STUDY; MALE; PRIMARY BILIARY CIRRHOSIS; PROTEIN BINDING;

EID: 77649241035     PISSN: 10079327     EISSN: None     Source Type: Journal    
DOI: 10.3748/wjg.v16.i8.973     Document Type: Article

References (43)

1. Berg PA, Klein R, Lindenborn-Fotinos J, Klöppel W. ATPase-associated antigen (M2): marker antigen for serological diagnosis of primary biliary cirrhosis. Lancet 1982; 2: 1423-1426
2. Lindenborn-Fotinos J, Sayers TJ, Berg PA. Mitochondrial antibodies in primary biliary cirrhosis. VI. Association of the complement fixing antigen with a component of the mitochondrial F1-ATPase complex. Clin Exp Immunol 1982; 50: 267-274
3. Berg PA, Muscatello U, Horne RW, Roitt IM, Doniach D. Mitochondrial antibodies in primary biliary cirrhosis. II. The complement fixing antigen as a component of mitochondrial inner membranes. Br J Exp Pathol 1969; 50: 200-208
4. Lindenborn-Fotinos J, Baum H, Berg PA. Mitochondrial antibodies in primary biliary cirrhosis: species and nonspecies specific determinants of M2 antigen. Hepatology 1985; 5: 763-769
5. Mackay IR, Whittingham S, Fida S, Myers M, Ikuno N, Gershwin ME, Rowley MJ. The peculiar autoimmunity of primary biliary cirrhosis. Immunol Rev 2000; 174: 226-237
6. Van de Water J, Gershwin ME, Leung P, Ansari A, Coppel RL. The autoepitope of the 74-kD mitochondrial autoantigen of primary biliary cirrhosis corresponds to the functional site of dihydrolipoamide acetyltransferase. J Exp Med 1988; 167: 1791-1799
7. Yeaman SJ. The 2-oxo acid dehydrogenase complexes: recent advances. Biochem J 1989; 257: 625-632
8. Fussey SP, Bassendine MF, James OF, Yeaman SJ. Characterisation of the reactivity of autoantibodies in primary biliary cirrhosis. FEBS Lett 1989; 246: 49-53
9. Surh CD, Coppel R, Gershwin ME. Structural requirement for autoreactivity on human pyruvate dehydrogenase-E2, the major autoantigen of primary biliary cirrhosis. Implication for a conformational autoepitope. J Immunol 1990; 144: 3367-3374
10. Fussey SP, Ali ST, Guest JR, James OF, Bassendine MF, Yeaman SJ. Reactivity of primary biliary cirrhosis sera with Escherichia coli dihydrolipoamide acetyltransferase (E2p): characterization of the main immunogenic region. Proc Natl Acad Sci USA 1990; 87: 3987-3991
11. Koike K, Ishibashi H, Koike M. Immunoreactivity of porcine heart dihydrolipoamide acetyl- and succinyl-transferases (PDC-E2, OGDC-E2) with primary biliary cirrhosis sera: characterization of the autoantigenic region and effects of enzymatic delipoylation and relipoylation. Hepatology 1998; 27: 1467-1474
12. Tuaillon N, Andre C, Briand JP, Penner E, Muller S. A lipoyl synthetic octadecapeptide of dihydrolipoamide acetyltransferase specifically recognized by anti-M2 autoantibodies in primary biliary cirrhosis. J Immunol 1992; 148: 445-450
13. Chen QY, Mackay IR, Fida S, Myers MA, Rowley MJ. Natural and disease associated autoantibodies to the autoantigen, dihydrolipoamide acetyltransferase, recognise different epitopes. J Autoimmun 1998; 11: 151-161
14. Rowley MJ, Scealy M, Whisstock JC, Jois JA, Wijeyewickrema LC, Mackay IR. Prediction of the immunodominant epitope of the pyruvate dehydrogenase complex E2 in primary biliary cirrhosis using phage display. J Immunol 2000; 164: 3413-3419
15. Amano K, Leung PS, Rieger R, Quan C, Wang X, Marik J, Suen YF, Kurth MJ, Nantz MH, Ansari AA, Lam KS, Zeniya M, Matsuura E, Coppel RL, Gershwin ME. Chemical xenobiotics and mitochondrial autoantigens in primary biliary cirrhosis: identification of antibodies against a common environmental, cosmetic, and food additive, 2-octynoic acid. J Immunol 2005; 174: 5874-5883
16. Berg PA, Doniach D, Roitt IM. Mitochondrial antibodies in primary biliary cirrhosis. I. Localization of the antigen to mitochondrial membranes. J Exp Med 1967; 126: 277-290
17. Klein R, Pointner H, Zilly W, Glässner-Bittner B, Breuer N, Garbe W, Fintelmann V, Kalk JF, Müting D, Fischer R, Tittor W, Pausch J, Maier KP, Berg PA. Antimitochondrial antibody profiles in primary biliary cirrhosis distinguish at early stages between a benign and a progressive course: a prospective study on 200 patients followed for 10 years. Liver 1997; 17: 119-128
18. Bellucci R, Oertelt S, Gallagher M, Li S, Zorn E, Weller E, Porcheray F, Alyea EP, Soiffer RJ, Munshi NC, Gershwin ME, Ritz J. Differential epitope mapping of antibodies to PDC-E2 in patients with hematologic malignancies after allogeneic hematopoietic stem cell transplantation and primary biliary cirrhosis. Blood 2007; 109: 2001-2007
19. Gershwin ME, Ansari AA, Mackay IR, Nakanuma Y, Nishio A, Rowley MJ, Coppel RL. Primary biliary cirrhosis: an orchestrated immune response against epithelial cells. Immunol Rev 2000; 174: 210-225
20. Yeaman SJ, Fussey SP, Danner DJ, James OF, Mutimer DJ, Bassendine MF. Primary biliary cirrhosis: identification of two major M2 mitochondrial autoantigens. Lancet 1988; 1: 1067-1070
21. Kilshaw PJ, McEwan FJ, Baker KC, Cant AJ. Studies on the specificity of antibodies to ovalbumin in normal human serum: technical considerations in the use of ELISA methods. Clin Exp Immunol 1986; 66: 481-489
22. Jois J, Omagari K, Rowley MJ, Anderson J, Mackay IR. Enzyme inhibitory antibody to pyruvate dehydrogenase: diagnostic utility in primary biliary cirrhosis. Ann Clin Biochem 2000; 37 (Pt 1): 67-73
23. Robertson CA, Coppel RL, Prindiville T, Fregeau D, Kaplan M, Dickson ER, Gershwin ME. The relative affinity of recombinant dihydrolipoamide transacetylase for autoantibodies in primary biliary cirrhosis. Hepatology 1990; 11: 717-722
24. Teoh KL, Mackay IR, Rowley MJ, Fussey SP. Enzyme inhibitory autoantibodies to pyruvate dehydrogenase complex in primary biliary cirrhosis differ for mammalian, yeast and bacterial enzymes: implications for molecular mimicry. Hepatology 1994; 19: 1029-1033
25. Van de Water J, Fregeau D, Davis P, Ansari A, Danner D, Leung P, Coppel R, Gershwin ME. Autoantibodies of primary biliary cirrhosis recognize dihydrolipoamide acetyltransferase and inhibit enzyme function. J Immunol 1988; 141: 2321-2324
26. Mattevi A, Obmolova G, Kalk KH, Westphal AH, de Kok A, Hol WG. Refined crystal structure of the catalytic domain of dihydrolipoyl transacetylase (E2p) from Azotobacter vinelandii at 2.6 A resolution. J Mol Biol 1993; 230: 1183-1199
27. Patel MS, Korotchkina LG. Regulation of the pyruvate dehydrogenase complex. Biochem Soc Trans 2006; 34: 217-222
28. Reed LJ, Hackert ML. Structure-function relationships in dihydrolipoamide acyltransferases. J Biol Chem 1990; 265: 8971-8974
29. Smolle M, Prior AE, Brown AE, Cooper A, Byron O, Lindsay JG. A new level of architectural complexity in the human pyruvate dehydrogenase complex. J Biol Chem 2006; 281: 19772-19780
30. Yu X, Hiromasa Y, Tsen H, Stoops JK, Roche TE, Zhou ZH. Structures of the human pyruvate dehydrogenase complex cores: a highly conserved catalytic center with flexible N-terminal domains. Structure 2008; 16: 104-114
31. Harris RA, Bowker-Kinley MM, Wu P, Jeng J, Popov KM. Dihydrolipoamide dehydrogenase-binding protein of the human pyruvate dehydrogenase complex. DNA-derived amino acid sequence, expression, and reconstitution of the pyruvate dehydrogenase complex. J Biol Chem 1997; 272: 19746-19751
32. Mattevi A, Obmolova G, Schulze E, Kalk KH, Westphal AH, de Kok A, Hol WG. Atomic structure of the cubic core of the pyruvate dehydrogenase multienzyme complex. Science 1992; 255: 1544-1550
33. Bogdanos DP, Dalekos GN. Enzymes as target antigens of liver-specific autoimmunity: the case of cytochromes P450s. Curr Med Chem 2008; 15: 2285-2292
34. Kiechle FL, Quattrociocchi-Longe TM, Brinton DA, Gordon SC, Sykes E, Elkhalifa MY. Autoantibodies to specific enzymes: a review. Ann Clin Lab Sci 1996; 26: 195-207
35. Bruggraber SF, Leung PS, Amano K, Quan C, Kurth MJ, Nantz MH, Benson GD, Van de Water J, Luketic V, Roche TE, Ansari AA, Coppel RL, Gershwin ME. Autoreactivity to lipoate and a conjugated form of lipoate in primary biliary cirrhosis. Gastroenterology 2003; 125: 1705-1713
36. Long SA, Quan C, Van de Water J, Nantz MH, Kurth MJ, Barsky D, Colvin ME, Lam KS, Coppel RL, Ansari A, Gershwin ME. Immunoreactivity of organic mimeotopes of the E2 component of pyruvate dehydrogenase: connecting xenobiotics with primary biliary cirrhosis. J Immunol 2001; 167: 2956-2963
37. Berg PA, Klein R. Mitochondrial antigen/antibody systems in primary biliary cirrhosis: revisited. Liver 1995; 15: 281-292
38. Jones DE. Pathogenesis of primary biliary cirrhosis. Clin Liver Dis 2008; 12: 305-321; viii
39. Xu L, Sakalian M, Shen Z, Loss G, Neuberger J, Mason A. Cloning the human betaretrovirus proviral genome from patients with primary biliary cirrhosis. Hepatology 2004; 39: 151-156
40. Rowley MJ, Maeda T, Mackay IR, Loveland BE, McMullen GL, Tribbick G, Bernard CC. Differing epitope selection of experimentally-induced and natural antibodies to a disease-specific autoantigen, the E2 subunit of pyruvate dehydrogenase complex (PDC-E2). Int Immunol 1992; 4: 1245-1253
41. Blundell TL, Sibanda BL, Sternberg MJ, Thornton JM. Knowledge-based prediction of protein structures and the design of novel molecules. Nature 1987; 326: 347-352
42. Horsfall AC, Hay FC, Soltys AJ, Jones MG. Epitope mapping. Immunol Today 1991; 12: 211-213
43. Mahler M, Blüthner M, Pollard KM. Advances in B-cell epitope analysis of autoantigens in connective tissue diseases. Clin Immunol 2003; 107: 65-79