A winding road to origin discovery

Chromosome Research

Volumn 18, Issue 1, 2010, Pages 45-61

  Hamlin, Joyce L ^a   Mesner, Larry D ^a   Dijkwel, Pieter A ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

2 D gels; DHFR; Mammalian replication origins; Nascent strands; Replication initiation

Indexed keywords

DIHYDROFOLATE REDUCTASE; DNA;

CHROMATIN STRUCTURE; DNA REPLICATION ORIGIN; GENE ACTIVITY; GENE CONTROL; GENE INACTIVATION; GENE LOCATION; GENE MAPPING; GENE REPLICATION; GENETIC ANALYSIS; GENETIC TRANSCRIPTION; GENOME; HUMAN; MAMMAL; MUTATION; NONHUMAN; PRIORITY JOURNAL; REVIEW; SACCHAROMYCES CEREVISIAE;

ANIMALS; CRICETINAE; CRICETULUS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; REPLICATION ORIGIN; TETRAHYDROFOLATE DEHYDROGENASE;

CRICETULUS GRISEUS; MAMMALIA; METAZOA;

EID: 77649232765     PISSN: 09673849     EISSN: 15736849     Source Type: Journal    
DOI: 10.1007/s10577-009-9089-z     Document Type: Review

References (86)

1. G Abdurashidova M Deganuto R Klima S Riva G Biamonti M Giacca A Falaschi 2000 Start sites of bidirectional DNA synthesis at the human lamin B2 origin Science 287 5460 2023 2026 10.1126/science.287.5460.2023 1:CAS:528: DC%2BD3cXhvF2qsL4%3D 10720330 (Pubitemid 30158680)
2. AL Altman E Fanning 2001 The Chinese hamster dihydrofolate reductase replication origin beta is active at multiple ectopic chromosomal locations and requires specific DNA sequence elements for activity Mol Cell Biol 21 4 1098 1110 10.1128/MCB.21.4.1098-1110.2001 1:CAS:528:DC%2BD3MXovVCgsA%3D%3D 11158297 (Pubitemid 32114959)
3. AL Altman E Fanning 2004 Defined sequence modules and an architectural element cooperate to promote initiation at an ectopic mammalian chromosomal replication origin Mol Cell Biol 24 10 4138 4150 10.1128/MCB.24.10.4138-4150. 2004 1:CAS:528:DC%2BD2cXjvFChtrg%3D 15121836 (Pubitemid 41070988)
4. B Anachkova JL Hamlin 1989 Replication in the amplified dihydrofolate reductase domain in CHO cells may initiate at two distinct sites. One of which is a repetitive sequence element Mol Cell Biol 9 532 540 1:CAS:528: DyaL1MXht12ksbw%3D 2710116
5. M Anglana F Apiou A Bensimon M Debatisse 2003 Dynamics of DNA replication in mammalian somatic cells: nucleotide pool modulates origin choice and interorigin spacing Cell 114 3 385 394 10.1016/S0092-8674(03)00569-5 1:CAS:528:DC%2BD3sXmsFWqt70%3D 12914702 (Pubitemid 36962936)
6. JL Biedler BA Spengler 1976 Metaphase chromosome anomaly: association with drug resistance and cell-specific products Science 191 4223 185 187 10.1126/science.942798 1:STN:280:DyaE287gsVGksw%3D%3D 942798
7. AK Bielinsky SA Gerbi 1998 Discrete start sites for DNA synthesis in the yeast ARS1 origin Science 279 95 98 10.1126/science.279.5347.95 1:CAS:528:DyaK1cXjt1OktA%3D%3D 9417033 (Pubitemid 28079163)
8. BJ Brewer WL Fangman 1987 The localization of replication origins on ARS plasmids in S. cerevisiae Cell 51 3 463 471 10.1016/0092-8674(87)90642-8 1:CAS:528:DyaL1cXjt1Omsw%3D%3D 2822257
9. C Brun PA Dijkwel RD Little JL Hamlin CL Schildkraut JA Huberman 1995 Yeast and mammalian replication intermediates migrate similarly in two-dimensional gels Chromosoma 104 2 92 102 10.1007/BF00347691 1:CAS:528:DyaK28Xjt1Sitg%3D%3D 8585995
10. WC Burhans JE Selegue NH Heintz 1986 Isolation of the origin of replication associated with the amplified Chinese hamster dihydrofolate reductase domain Proc Natl Acad Sci U S A 83 7790 7794 10.1073/pnas.83.20.7790 1:CAS:528:DyaL28Xmt1Sisr0%3D 3094015 (Pubitemid 17183930)
11. WC Burhans LT Vassilev MS Caddle NH Heintz ML Depamphilis 1990 Identification of an origin of bidirectional DNA replication in mammalian chromosomes Cell 62 5 955 965 10.1016/0092-8674(90)90270-O 1:CAS:528: DyaK3cXlvVGrtL4%3D 2393905 (Pubitemid 20269142)
12. WC Burhans LT Vassilev J Wu JM Sogo FS Nallaseth ML Depamphilis 1991 Emetine allows identification of origins of mammalian DNA replication by imbalanced DNA synthesis, not through conservative nucleosome segregation EMBO J 10 13 4351 4360 1:CAS:528:DyaK38Xlt1Ohug%3D%3D 1721870
13. JC Cadoret F Meisch V Hassan-Zadeh I Luyten C Guillet L Duret H Quesneville MN Prioleau 2008 Genome-wide studies highlight indirect links between human replication origins and gene regulation Proc Natl Acad Sci U S A 105 41 15837 15842 10.1073/pnas.0805208105 1:CAS:528:DC%2BD1cXht1yitrnO 18838675
14. CS Chan BK Tye 1980 Autonomously replicating sequences in Saccharomyces cerevisiae Proc Natl Acad Sci U S A 77 6329 6333 10.1073/pnas.77.11.6329 1:CAS:528:DyaL3MXislamsQ%3D%3D 7005897 (Pubitemid 11219141)
15. PA Dijkwel JL Hamlin 1992 Initiation of DNA replication in the dihydrofolate reductase locus is confined to the early S period in CHO cells synchronized with the plant amino acid mimosine Mol Cell Biol 12 9 3715 3722 1:CAS:528:DyaK38Xls1Ghsrc%3D 1508178
16. PA Dijkwel JL Hamlin 1995 The Chinese hamster dihydrofolate reductase origin consists of multiple potential nascent-strand start sites Mol Cell Biol 15 6 3023 3031 1:CAS:528:DyaK2MXlslyit7o%3D 7760799
17. PA Dijkwel JP Vaughn JL Hamlin 1991 Mapping of replication initiation sites in mammalian genomes by two-dimensional gel analysis: stabilization and enrichment of replication intermediates by isolation on the nuclear matrix Mol Cell Biol 11 8 3850 3859 1:CAS:528:DyaK3MXlt12jurY%3D 2072896
18. PA Dijkwel JP Vaughn JL Hamlin 1994 Replication initiation sites are distributed widely in the amplified CHO dihydrofolate reductase domain Nucleic Acids Res 22 23 4989 4996 10.1093/nar/22.23.4989 1:CAS:528:DyaK2MXisl2msr0%3D 7800491
19. PA Dijkwel LD Mesner VV Levenson J D'anna JL Hamlin 2000 Dispersive initiation of replication in the Chinese hamster rhodopsin locus Exp Cell Res 256 1 150 157 10.1006/excr.2000.4809 1:CAS:528:DC%2BD3cXitVWqtb0%3D 10739662 (Pubitemid 30211177)
20. PA Dijkwel S Wang JL Hamlin 2002 Initiation sites are distributed at frequent intervals in the Chinese hamster dihydrofolate reductase origin of replication but are used with very different efficiencies Mol Cell Biol 22 9 3053 3065 10.1128/MCB.22.9.3053-3065.2002 1:CAS:528:DC%2BD38XivFylsbc%3D 11940663 (Pubitemid 34437464)
21. L Frappier M Zannis-Hadjopoulos 1987 Autonomous replication of plasmids bearing monkey DNA origin-enriched sequences Proc Natl Acad Sci U S A 84 6668 6672 10.1073/pnas.84.19.6668 1:CAS:528:DyaL2sXlvFaiu7c%3D 3477798
22. M Giacca L Zentilin P Norio S Diviacco D Dimitrova G Contreas G Biamonti G Perini F Weighardt S Riva 1994 Fine mapping of a replication origin of human DNA Proc Natl Acad Sci U S A 91 15 7119 7123 10.1073/pnas.91.15.7119 1:CAS:528:DyaK2cXlt1Klur4%3D 8041756 (Pubitemid 24226814)
23. DM Gilbert A Neilson H Miyazawa ML Depamphilis WC Burhans 1995 Mimosine arrests DNA synthesis at replication forks by inhibiting deoxyribonucleotide metabolism J Biol Chem 270 16 9597 9606 10.1074/jbc.270.16.9413 1:CAS:528:DyaK2MXlt1aju7s%3D 7721891
24. M Gomez F Antequera 2008 Overreplication of short DNA regions during S phase in human cells Genes Dev 22 3 375 385 10.1101/gad.445608 1:CAS:528:DC%2BD1cXit12iurw%3D 18245449 (Pubitemid 351214278)
25. SJ Gray G Liu AL Altman LE Small E Fanning 2007 Discrete functional elements required for initiation activity of the Chinese hamster dihydrofolate reductase origin beta at ectopic chromosomal sites Exp Cell Res 313 1 109 120 10.1016/j.yexcr.2006.09.020 1:CAS:528:DC%2BD2sXhsFCmtQ%3D%3D 17078947 (Pubitemid 44958457)
26. JL Hamlin AB Pardee 1976 S phase synchrony in monolayer CHO cultures Exp Cell Res 100 2 265 275 10.1016/0014-4827(76)90147-6 1:CAS:528:DyaE28XkvFWgtLs%3D 939252
27. JL Hamlin LD Mesner O Lar R Torres SV Chodaparambil L Wang 2008 A revisionist replicon model for higher eukaryotic genomes J Cell Biochem 105 2 321 329 10.1002/jcb.21828 1:CAS:528:DC%2BD1cXht1elu73L 18680119
28. S Handeli A Klar M Meuth H Cedar 1989 Mapping replication units in animal cells Cell 57 6 909 920 10.1016/0092-8674(89)90329-2 1:CAS:528: DyaL1MXksFWmtbk%3D 2544294 (Pubitemid 19168371)
29. JW Harper SJ Elledge 2007 The DNA damage response: ten years after Mol Cell 28 5 739 745 10.1016/j.molcel.2007.11.015 1:CAS:528:DC%2BD1cXktVWisQ%3D%3D 18082599 (Pubitemid 350217064)
30. RT Hay ML Depamphilis 1982 Initiation of SV40 DNA replication in vivo: location and structure of 5′ ends of DNA synthesized in the Ori region Cell 28 767 779 10.1016/0092-8674(82)90056-3 1:CAS:528:DyaL38XitFWksLg%3D 6178514
31. NH Heintz JL Hamlin 1982 An amplified chromosomal sequence that includes the gene for dihydrofolate reductase initiates replication within specific restriction fragments Proc Natl Acad Sci U S A 79 13 4083 4087 10.1073/pnas.79.13.4083 1:CAS:528:DyaL38Xks1Oisbo%3D 6955792 (Pubitemid 12035082)
32. NH Heintz JL Hamlin 1983 In vivo effects of cytosine arabinoside on deoxyribonucleic acid replication in Chinese hamster ovary cells. 1 Resolution of differential effects on mitochondrial and nuclear deoxyribonucleic acid synthesis Biochemistry 22 15 3552 3557 10.1021/bi00284a003 1:CAS:528: DyaL3sXksVOls7o%3D 6615783
33. NH Heintz JL Hamlin 1983 In vivo effects of cytosine arabinoside on deoxyribonucleic acid replication in Chinese hamster ovary cells. 2 Cytosine arabinoside affects the rate of synthesis but not the pattern of labeling of an amplified chromosomal sequence at the onset of the S period Biochemistry 22 15 3557 3562 10.1021/bi00284a004 1:CAS:528:DyaL3sXksVOls7s%3D 6615784
34. NH Heintz JD Milbrandt KS Greisen JL Hamlin 1983 Cloning of the initiation region of a mammalian chromosomal replicon Nature 302 5907 439 441 10.1038/302439a0 1:CAS:528:DyaL3sXitFSrsrw%3D 6300683 (Pubitemid 13164333)
35. SS Heinzel PJ Krysan CT Tran MP Calos 1991 Autonomous DNA replication in human cells is affected by the size and the source of the DNA Mol Cell Biol 11 2263 2272 1:CAS:528:DyaK3MXit1amu7s%3D 1900922
36. J Herrick A Bensimon 2009 Introduction to molecular combing: genomics DNA replication, and cancer Methods Mol Biol 521 71 101 1:CAS:528: DC%2BD1MXls1ersbg%3D 19563102
37. A Holst F Muller G Zastrow H Zentgraf S Schwender E Dinkl F Grummt 1988 Murine genomic DNA sequences replicating autonomously in mouse L cells Cell 52 355 365 10.1016/S0092-8674(88)80028-X 1:CAS:528:DyaL1cXhs1amtbY%3D 2830982
38. JA Huberman 1981 New views of the biochemistry of eucaryotic DNA replication revealed by aphidicolin, an unusual inhibitor of DNA polymerase alpha Cell 23 3 647 648 10.1016/0092-8674(81)90426-8 1:CAS:528: DyaL3MXhs12msLk%3D 6784928
39. JA Huberman AD Riggs 1968 On the mechanism of DNA replication in mammalian chromosomes J Mol Biol 32 2 327 341 10.1016/0022-2836(68)90013-2 1:CAS:528:DyaF1cXptlSiug%3D%3D 5689363
40. F Jacob S Brenner 1963 On the regulation of DNA synthesis in bacteria: the hypothesis of the replicon C R Hebd Seances Acad Sci 256 298 300 1:CAS:528:DyaF3sXktFKgsg%3D%3D 13964280
41. Y Jin TA Yie AM Carothers 1995 Non-random deletions at the dihydrofolate reductase locus of Chinese hamster ovary cells induced by alpha-particles simulating radon Carcinogenesis 16 8 1981 1991 10.1093/carcin/16.8.1981 1:CAS:528:DyaK2MXns1SrsL8%3D 7634430
42. RF Kalejta X Li LD Mesner PA Dijkwel HB Lin JL Hamlin 1998 Distal sequences but not Ori-beta/Obr-1, are essential for initiation of DNA replication in the Chinese hamster DHFR origin Mol Cell 2 797 806 10.1016/S1097-2765(00)80294-4 1:CAS:528:DyaK1MXjslaitw%3D%3D 9885567
43. G Kaufmann HM Zannis RG Martin 1985 Cloning of nascent monkey DNA synthesized early in the cell cycle Mol Cell Biol 5 721 727 1:CAS:528: DyaL2MXitFKht7s%3D 3990692
44. T Kobayashi T Rein ML Depamphilis 1998 Identification of primary initiation sites for DNA replication in the hamster dihydrofolate reductase gene initiation zone Mol Cell Biol 18 3266 3277 1:CAS:528:DyaK1cXjsFSku7s%3D 9584167
45. PJ Krysan SB Haase MP Calos 1989 Isolation of human sequences that replicate autonomously in human cells Mol Cell Biol 9 3 1026 1033 1:CAS:528:DyaL1MXhsV2is74%3D 2542763
46. Y Kurosawa T Ogawa S Hirose T Okazaki R Okazaki 1975 Mechanism of DNA chain growth. Xv. RNA-linked nascent DNA pieces in Escherichia coli strains assayed with spleen exonuclease J Mol Biol 96 4 653 664 10.1016/0022-2836(75) 90144-8 1:CAS:528:DyaE2MXls1Smtbc%3D 1104864
47. MM Lalande 1990 A reversible arrest point in the late G1 phase of the mammalian cell cycle Exp Cell Res 186 332 339 10.1016/0014-4827(90)90313-Y 1:CAS:528:DyaK3cXhtFKntrc%3D 2153561
48. M Leffak CD James 1989 Opposite replication polarity of the germ line c-Myc gene in hela cells compared with that of two Burkitt lymphoma cell lines Mol Cell Biol 9 2 586 593 1:CAS:528:DyaL1MXht12ksbo%3D 2710118
49. TH Leu JL Hamlin 1989 High-resolution mapping of replication fork movement through the amplified dihydrofolate reductase domain in CHO cells by in-gel renaturation analysis Mol Cell Biol 9 2 523 531 1:CAS:528: DyaL1MXht12ksb8%3D 2710115
50. TH Leu B Anachkova JL Hamlin 1990 Repetitive sequence elements in an initiation locus of the amplified dihydrofolate reductase domain in CHO cells Genomics 7 3 428 433 10.1016/0888-7543(90)90178-W 1:CAS:528:DyaK3MXhvVChsw%3D%3D 2365359
51. HB Lin PA Dijkwel JL Hamlin 2005 Promiscuous initiation on mammalian chromosomal DNA templates and its possible suppression by transcription Exp Cell Res 308 53 64 10.1016/j.yexcr.2005.04.012 1:CAS:528:DC%2BD2MXmt1ejt70%3D 15904920 (Pubitemid 40984761)
52. RD Little TH Platt CL Schildkraut 1993 Initiation and termination of DNA replication in human RRNA genes Mol Cell Biol 13 10 6600 6613 1:CAS:528:DyaK3sXms1Cjurg%3D 8413256
53. G Liu M Malott M Leffak 2003 Multiple functional elements comprise a mammalian chromosomal replicator Mol Cell Biol 23 5 1832 1842 10.1128/MCB.23.5.1832-1842.2003 1:CAS:528:DC%2BD3sXhs1SgsLo%3D 12589000 (Pubitemid 36246062)
54. JE Looney JL Hamlin 1987 Isolation of the amplified dihydrofolate reductase domain from methotrexate-resistant Chinese hamster ovary cells Mol Cell Biol 7 569 577 1:CAS:528:DyaL2sXhsFSmsb8%3D 3821723
55. C Ma TH Leu JL Hamlin 1990 Multiple origins of replication in the dihydrofolate reductase amplicons of a methotrexate-resistant Chinese hamster cell line Mol Cell Biol 10 4 1338 1346 1:CAS:528:DyaK3cXhslems7c%3D 2320001
56. RC Marsh A Worcel 1977 A DNA fragment containing the origin of replication of the Escherichia coli chromosome Proc Natl Acad Sci U S A 74 2720 2724 10.1073/pnas.74.7.2720 1:CAS:528:DyaE2sXkvFylu7o%3D 268621 (Pubitemid 8150807)
57. LD Mesner JL Hamlin 2005 Specific signals at the 3′ end of the DHFR gene define one boundary of the downstream origin of replication Genes Dev 19 9 1053 1066 10.1101/gad.1307105 1:CAS:528:DC%2BD2MXkt1Cgsrs%3D 15879555 (Pubitemid 40632451)
58. LD Mesner X Li PA Dijkwel JL Hamlin 2003 The dihydrofolate reductase origin of replication does not contain any nonredundant genetic elements required for origin activity Mol Cell Biol 23 3 804 814 10.1128/MCB.23.3.804- 814.2003 1:CAS:528:DC%2BD3sXnvFGhsg%3D%3D 12529386 (Pubitemid 36133499)
59. LD Mesner EL Crawford JL Hamlin 2006 Isolating apparently pure libraries of replication origins from complex genomes Mol Cell 21 5 719 726 10.1016/j.molcel.2006.01.015 1:CAS:528:DC%2BD28XislKqsLc%3D 16507369 (Pubitemid 43290738)
60. JD Milbrandt NH Heintz WC White SM Rothman JL Hamlin 1981 Methotrexate-resistant Chinese hamster ovary cells have amplified a 135-kilobase-pair region that includes the dihydrofolate reductase gene Proc Natl Acad Sci U S A 78 10 6043 6047 10.1073/pnas.78.10.6043 1:CAS:528: DyaL3MXmtVWgsro%3D 6273843
61. J Mirkovitch ME Mirault UK Laemmli 1984 Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold Cell 39 223 232 10.1016/0092-8674(84)90208-3 1:CAS:528:DyaL2MXhvVOqtw%3D%3D 6091913
62. PJ Mosca PA Dijkwel JL Hamlin 1992 The plant amino acid mimosine may inhibit initiation at origins of replication in Chinese hamster cells Mol Cell Biol 12 10 4375 4383 1:CAS:528:DyaK3sXhvF2qtg%3D%3D 1406627 Published erratum appears in Mol Cell Biol 1993, 13:1981
63. KA Nawotka JA Huberman 1988 Two-dimensional gel electrophoretic method for mapping DNA replicons Mol Cell Biol 8 4 1408 1413 1:CAS:528: DyaL1cXhvVWnsb8%3D 2837639
64. R Okazaki S Hirose T Okazaki T Ogawa Y Kurosawa 1975 Assay of RNA-linked nascent DNA pieces with polynucleotide kinase Biochem Biophys Res Commun 62 4 1018 1024 10.1016/0006-291X(75)90424-6 1:CAS:528:DyaE2MXht1Wgtro%3D 164190
65. S Paixao IN Colaluca M Cubells FA Peverali A Destro S Giadrossi M Giacca A Falaschi S Riva G Biamonti 2004 Modular structure of the human lamin B2 replicator Mol Cell Biol 24 7 2958 2967 10.1128/MCB.24.7.2958-2967.2004 1:CAS:528:DC%2BD2cXis1emtrk%3D 15024083 (Pubitemid 38381284)
66. C Pelizon S Diviacco A Falaschi M Giacca 1996 High-resolution mapping of the origin of DNA replication in the hamster dihydrofolate reductase gene domain by competitive PCR Mol Cell Biol 16 5358 5364 1:CAS:528:DyaK28XlvFamtL4%3D 8816447
67. DM Prescott PL Kuempel 1972 Bidirectional replication of the chromosome in Escherichia coli Proc Natl Acad Sci U S A 69 10 2842 2845 10.1073/pnas.69.10.2842 1:CAS:528:DyaE3sXhvFCn 4562743
68. IB Roninson 1983 Detection and mapping of homologous repeated and amplified DNA sequences by DNA renaturation in agarose gels Nucleic Acids Res 11 5413 5431 10.1093/nar/11.16.5413 1:CAS:528:DyaL3sXlvFSrsrw%3D 6310499
69. GE Roth HM Blanton LJ Hager VA Zakian 1983 Isolation and characterization of sequences from mouse chromosomal DNA with ARS function in yeasts Mol Cell Biol 3 11 1898 1908 1:CAS:528:DyaL3sXmtVOgsb8%3D 6361521
70. DJ Roufa MA Marchionni 1982 Nucleosome segregation at a defined mammalian chromosomal site Proc Natl Acad Sci U S A 79 6 1810 1814 10.1073/pnas.79.6.1810 1:CAS:528:DyaL38Xhs1Sjs78%3D 6281787 (Pubitemid 12088517)
71. S Saha Y Shan LD Mesner JL Hamlin 2004 The promoter of the Chinese hamster ovary dihydrofolate reductase gene regulates the activity of the local origin and helps define its boundaries Genes Dev 18 4 397 410 10.1101/gad.1171404 1:CAS:528:DC%2BD2cXitlWnu78%3D 14977920 (Pubitemid 38316347)
72. T Sasaki S Ramanathan Y Okuno C Kumagai SS Shaikh DM Gilbert 2006 The Chinese hamster dihydrofolate reductase replication origin decision point follows activation of transcription and suppresses initiation of replication within transcription units Mol Cell Biol 26 3 1051 1062 10.1128/MCB.26.3.1051- 1062.2006 1:CAS:528:DC%2BD28XhtVOns78%3D 16428457 (Pubitemid 43146497)
73. C Schurra A Bensimon 2009 Combing genomic DNA for structural and functional studies Methods Mol Biol 464 71 90 10.1007/978-1-60327-461-6-5 18951180
74. MM Seidman AJ Levine H Weintraub 1979 The asymmetric segregation of parental nucleosomes during chromosome replication Cell 18 2 439 449 10.1016/0092-8674(79)90063-1 1:CAS:528:DyaL3cXhslGj 227608 (Pubitemid 10248368)
75. DT Stinchcomb M Thomas J Kelly E Selker RW Davis 1980 Eukaryotic DNA segments capable of autonomous replication in yeast Proc Natl Acad Sci U S A 77 4559 4563 10.1073/pnas.77.8.4559 1:CAS:528:DyaL3cXmtVSrsbg%3D 6449009
76. RA Tobey 1973 Production and characterization of mammalian cells reversibly arrested in G1 by growth in isoleucine-deficient medium Methods Cell Biol 6 67 112 10.1016/S0091-679X(08)60048-5 1:STN:280:DyaE2c%2Fjt1yjtQ%3D%3D 4585084
77. C Tribioli G Biamonti M Giacca M Colonna S Riva A Falaschi 1987 Characterization of human DNA sequences synthesized at the onset of S-phase Nucleic Acids Res 15 24 10211 10232 10.1093/nar/15.24.10211 1:CAS:528: DyaL1cXovVygtw%3D%3D 2827117
78. G Urlaub PJ Mitchell E Kas LA Chasin VL Funanage TT Myoda JL Hamlin 1986 Effect of gamma rays at the dihydrofolate reductase locus: deletions and inversions Somat Cell Mol Genet 12 6 555 566 10.1007/BF01671941 1:CAS:528:DyaL2sXks1Ggtw%3D%3D 3024331 (Pubitemid 17188077)
79. LT Vassilev EM Johnson 1989 Mapping initiation sites of DNA replication in vivo using polymerase chain reaction amplification of nascent strand segments Nucleic Acids Res 17 7693 7705 10.1093/nar/17.19.7693 1:CAS:528: DyaL1MXmt1Kmu70%3D 2552413
80. LT Vassilev WC Burhans ML Depamphilis 1990 Mapping an origin of DNA replication at a single-copy locus in exponentially proliferating mammalian cells Mol Cell Biol 10 4685 4689 1:CAS:528:DyaK3cXls1KlsLo%3D 2388621
81. JP Vaughn PA Dijkwel JL Hamlin 1990 Replication initiates in a broad zone in the amplified CHO dihydrofolate reductase domain Cell 61 6 1075 1087 10.1016/0092-8674(90)90071-L 1:STN:280:DyaK3c3nsFKjsQ%3D%3D 2350784 (Pubitemid 20203490)
82. JP Vaughn PA Dijkwel LH Mullenders JL Hamlin 1990 Replication Forks are associated with the nuclear matrix Nucleic Acids Res 18 8 1965 1969 10.1093/nar/18.8.1965 1:CAS:528:DyaK3cXktlKmt78%3D 2336385
83. K Von Meyenburg FG Hansen LD Nielsen E Riise 1978 Origin of replication oriC, of the E. coli chromosome on specialized transducing phages lambda-Asn Mol Gen Genet 160 287 295 10.1007/BF00332972
84. S Wang PA Dijkwel JL Hamlin 1998 Lagging-strand early-labelling, and two-dimensional gel assays suggest multiple potential initiation sites in the Chinese hamster dihydrofolate reductase origin Mol Cell Biol 18 39 50 9418851
85. L Wang CM Lin S Brooks D Cimbora M Groudine MI Aladjem 2004 The human beta-globin replication initiation region consists of two modular independent replicators Mol Cell Biol 24 8 3373 3386 10.1128/MCB.24.8.3373-3386.2004 1:CAS:528:DC%2BD2cXjtFKjt7o%3D 15060158 (Pubitemid 38452080)
86. S Yasuda Y Hirota 1977 Cloning and mapping of the replication origin of Escherichia coli Proc Natl Acad Sci U S A 74 5458 5462 10.1073/pnas.74.12.5458 1:CAS:528:DyaE1cXosFyltw%3D%3D 341158