Protein synthesis in hepatocytes of mice as revealed by electron microscopic radioautography

Protein Biosynthesis

Volumn , Issue , 2009, Pages 133-162

  Nagata, Tetsuji ^a,b  

^a SHINSHU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b Shinshu Institute of Alternative Medicine and Welfare   (Japan)

Author keywords

Aging; EM radioautography; Mitochondria; Mouse hepatocytes; Protein syntheses

Indexed keywords

EID: 77649181294     PISSN: None     EISSN: None     Source Type: Book    
DOI: None     Document Type: Chapter

References (100)

1. Nagata T, Shibata O, Nawa T. (1967). Electron microscopic radioautographic studies of nucleic acid and protein syntheses in the liver and kidney cells of chickens and mice in tissue culture. Arch Histol Japon 28: 540-541.
2. Nagata T, Shibata O, Nawa T. (1967). Incorporation of tritiated thymidine into mitochondrial DNA of the liver and kidney cells of chickens and mice in tissue culture. Histochemie 10: 305-308.
3. Nagata T. (1969). Electron microscopic radioautography of intramitochondrial RNA synthesis of HeLa cells in culture. Acta Anat Nippon 44: 126-127.
4. Nagata T. (1972). Electron microscopic dry-mounting autoradiography. Proc. 4th Internat Cong Histochem Cytochem, Jap Soc Histochem Cytochem ed. Kyoto, pp. 43-44.
5. Nagata T. (1972). Radioautographic study on intramitochondrial nucleic acid synthesis: Its relationship to the cell cycle in cultivated cells. Proc. 4th Internat Cong Histochem Cytochem, Jap Soc Histochem Cytochem ed. Kyoto, 223-224.
6. Nagata T. (1972). Electron microscopic radioautography of intramitochondrial RNA synthesis of HeLa cells in culture. Histochemie 32: 163-170.
7. Nagata T. (1972). Quantitative electron microscope radioautography of intramitochondrial nucleic acid synthesis. Acta Histochem Cytochem 5: 201-203.
8. Nagata T. (1974) Electron microscopic radioautography of intramitochondrial nucleic acid syntheses in mammalian cells in vitro. Proc. 8th Internat Cong Electron Microsc, Australia Electron Microsc Soc ed. Canberra, Vol.II, pp. 346-347.
9. Nagata T, Yamada Y, Iwadare N, Murata F. (1975). Relationship of intramitochondrial nucleic acid synthesis to the nucleoli in cultivated cells as revealed by electron microscopic radioautography. Proc 10th Internat Cong Anat, Jap Assoc Anat ed. Tokyo, pp. 474-475.
10. Nagata T, Iwadare, N, Murata F. (1976). Mitochondrial and nucleolar RNA synthesis as revealed by electron microscopic radioautography. Proc 5th Internat Cong Histochem Cytochem, Rumanian Histochem Soc ed. Bucharest, pp. 242-243.
11. Nagata T, Iwadare N, Murata F. (1977). Electron microscopic radioautography of nucleic acid synthesis in cultured cells treated with several carcinogens. Acta Pharmacol Toxicol 41: 64-65.
12. Nagata T, Murata F. (1977). Electron microscopic dry-mounting radioautography for diffusible compounds by means of ultracryotomy. Histochemistry 54: 75-82.
13. Nagata T, Murata F, Yoshida K, Ohno S, Iwadare N. (1977). Whole mount radioautography of cultured cells as observed by high voltage electron microscopy. Proc. 5th Internat Conf High Voltage Electron Microsc, Jap Electron Micrsc Soc ed. Kyoto, pp. 347-350.
14. Nagata T, Ohno S, Murata F. (1977). Electron microscopic dry-mounting radioautography for soluble compounds. Acta Pharmacol. Toxicol. 41: 62-63.
15. Nagata T. (1984). Electron microscopic observation of target cells previously observed by phase-contrast microscopy: Electron microscopic radioautography of laser beam irradiated cultured cells. J Clin Electron Microsc 17: 589-590.
16. Nagata T. (1992). Radiolabeling of soluble and insoluble compounds as demonstrated by light and electron microscopy. Recent Advances in Cellular and Molecular Biology, Wegmann RJ, Wegmann MA eds. Peters Press, Leuven, Vol. 6: pp.9-21.
17. Nagata T, Kawahara I. (1999). Radioautographic study of the synthesis of sulfomucin in digestive organs of mice. J. Trace Microprobe Analysis. 17: 339-355.
18. Nagata T, Kawahara I, Usuda N, Maruyama M, Ma H. (1988). Radioautographic studies on the glycoconjugates synthesis in the gastrointestinal mucosa of the mouse. In, Glycoconjugates in Medicine. Ohyama M ad Muramatsu T. (eds.) Profes. Postgrad. Service, Tokyo, pp. 251-256.
19. Nagata, T. (1993). Quantitative light and electron microscopic radioautographic studies on macromolecular synthesis in several organs of prenatal and postnatal aging mice. Chin J Histochem Cytochem 2: 106-108.
20. Nagata T. (1995) Light and electron microscopic radioautographic studies on macromolecular synthesis in digestive organs of aging mice. Cell Mol Biol 41: 21-38.
21. Nagata T. (1999). Aging changes of macromolecular synthesis in various organs as observed by microscopic radioautography after incorporation of radiolabeled precursors. Methods Find Exp Clin Pharmacol 21: 683-706.
22. Nagata T. (1994). Radioautography in Medicine. Shinshu Univ Press, Matsumoto, Japan, pp. 1-268.
23. Nagata T. (1996). Techniques and application of electron microscopic radioautography. J Electron Microsc 45: 258-274.
24. Nagata T. (1997). Techniques and application of microscopic radioautography. Histol Histopathol 12: 1091-1124.
25. Nagata T, Ito M, Chen S. (2000). Aging changes of DNA synthesis in the submandibular glands of mice as observed by light and electron microscopic radioautography. Ann Microsc 1: 13-22.
26. Nagata T. (1962). A radioautographic study of the DNA synthesis in rat liver with special reference to binucleate cells. Med J Shinshu Univ 7: 17-25.
27. Nagata T, Ohno S, Kawahara I, Yamabayashi S, Fujii Y, Murata F. (1979). Light and electron microscopic radioautography of nucleic acid synthesis in mitochondria and peroxisomes of rat hepatic cells during and after DEHP administration. Acta Histochem Cytochem 16: 610-611.
28. Nagata T, Fujii Y, Usuda N. (1982). Demonstration of extranuclear nucleic acid synthesis in mammalian cells under experimental conditions by electron microscopic radioautography. Proc. 10th Internat Cong Electron Microsc, German Electron Microsc Soc ed. Hamburg, Vol 2: 305-306.
29. Nagata T, Ohno S, Yoshida K, Murata F. (1982). Nucleic acid synthesis in proliferating peroxisomes of rat liver as revealed by electron microscopical radioautography. Histochem J 14: 197-204.
30. Ma H, Nagata T. (1988). Studies on DNA synthesis of aging mice by means of electron microscopic radioautography. J Clin Electron Microsc 21: 335-343.
31. Ma H, Nagata T. (1988). Electron microscopic radioautographic study of DNA synthesis in the livers of aging mice. J Clin Electron Microsc 21: 715-716.
32. Ma H, Nagata T. (1990). Electron microscopic radioautographic studies on DNA synthesis in the hepatocytes of aging mice as observed by image analysis. Cell Mol Biol 36: 73-84.
33. Ma H, Gao F, Olea MT, Nagata T. (1991). Protein synthesis in the livers of aging mice studied by electron microscopic radioautography. Cell Mol Biol 37: 607-615.
34. Ma H, Gao F, Sun L, Jin C, Nagata T. (1994). Electron microscopic radioautographic study on the synthesis of DNA, RNA and protein in the livers of aging mice. Med Electron Microsc 27: 349-351.
35. Ma H, Nagata T. (2000). Collagen and protein synthesis in the livers of aging mice as studied by electron microscopic radioautography. Ann Microsc 1: 13-22
36. Nagata T, Ma H. (2003). Electron microscopic radioautographic study on the macromolecular synthesis in binucleate hepatocytes of aging mouse. Ann Microsc 3: 53-68.
37. Nagata T. (2003). Light and electron microscopic radioautographic studies on macromolecular synthesis in amitotic hepatocytes of aging mice. Cell Mol Biol 49: 591-611.
38. Nagata T, Ma H. (2003). Electron microscopic radioautographic study on nucleic acid synthesis in amitotic hepatocytes of the aging mouse. Med Electron Microsc 36: 263-271.
39. Nagata T, Ma H. (2004). Electron microscopic radioautographic study on protein synthesis in amitotic hepatocytes of the aging mouse. Med Electron Microsc 37: 62-69.
40. Nagata T, Ma H. (2005). Electron microscopic radioautographic study on mitochondrial DNA synthesis in hepatocytes of aging mouse. Ann Microsc 5: 4-18.
41. Nagata T, Ma H. (2005). Electron microscopic radioautographic study on RNA synthesis in hepatocytes of aging mouse. Microsc Res Tech 64: 55-64.
42. Nagata T. (2006). Electron microscopic radioautographic study on protein synthesis in hepatocyte mitochondria of developing mice. Ann. Microsc. 6: 43-54.
43. T. Nagata (2006) Electron microscopic radioautographic study on protein synthesis in hepatocyte mitochondria of aging mice. The Sci World J. 6: 1583-1598.
44. Nagata T, Usuda N, Ma H. (1986). Electron microscopic radioautography of nucleic acid synthesis in pancreatic acinar cells of prenatal and postnatal aging mice. Proc. 11th Internat Cong Electron Microsc, Jap Electron Microsc Soc ed. Kyoto, Vol 3: 2281-2282.
45. Nagata T. (2000). Light and electron microscopic radioautographic study on the protein synthesis in the pancreas of aging mouse. Bull Nagano Women's Jr Coll 8: 1-26
46. Sun L, Gao F, Jin C, Nagata T. (1997). DNA synthesis in the trachea of aging mice by light and electron microscopic radioautography. Acta Histochem Cytochem 30: 211-220.
47. Hanai T, Nagata T. (1994). Electron microscopic radioautographic study on nucleic acid synthesis in perinatal mouse kidney tissue. Med Electron Microsc 27: 355-357.
48. Gao F, Ma H, Sun L, Jin C, Nagata T. (1994). Electron microscopic radioautographic study on the nucleic acid and protein synthesis in the aging mouse testis. Med Electron Microsc 27: 360-362.
49. Gao F, Chen S, Sun L, Kang W, Wang Z, Nagata T. (1995). Radioautographic study of the macromolecular synthesis of Leydig cells in aging mouse testis. Cell. Mol. Biol. 41: 145-150.
50. Yamada AT, Nagata T. (1992). Light and electron microscopic radioautography of DNA synthesis in the endometria of pregnant ovariectomized mice during activation of implantation window. Cell Mol Biol 38: 763-774.
51. Yamada AT. (1993). Timely and topographically defined protein synthesis in the peri-implanting mouse endometrium revealed by light and electron microscopic radioautography. Cell Mol Biol 39: 1-12.
52. Yamada AT, Nagata T. (1993). Light and electron microscopic radioautographic studies on the DNA synthesis of peri-implanting pregnant mouse uterus during activation of receptivity for blastocyst implantation. Cell Mol Biol 39: 221-233.
53. Yamada AT, Nagata T. (1994). Ribonucleic acid and protein synthesis in the uterus of pregnant mouse during activation of implantation window. Med Electron Microsc 27:
54. Olea MT, Nagata T. (2003). Application of high voltage electron microscopy to biological specimens: A study on the protein synthesis in aging mouse spleen labeled with 3H-leucine. Ann Microsc 3: 70-84.
55. Olea MT, Nagata T. (2006). Electron microscopic radioautographic study on protein synthesis in mouse spleen in aging. Ann Microsc 6: 55-66.
56. Ito M, Nagata T. (1996). Electron microscopic radioautographic study on DNA synthesis and the ultrastructure of the adrenal gland in aging mice. Med Electron Microsc 29: 145-152.
57. Liang Y, Ito M, Nagata T. (1999). Light and electron microscopic radioautographic studies on RNA synthesis in aging mouse adrenal gland. Acta Anat Nippon 74: 291-300.
58. Cui H, Gao F, Ma H, Nagata T. (1996). Study on DNA synthesis of cellular elements in the cerebella of aging mice by light and electron microscopic radioautography. Proc 4th China-Japan Joint Histochem Cytochem Symp. Chinese Histochem Soc ed. Chongqing Publishing House, Chongqing, pp. 111-112.
59. Gunarso W. (1984). Radioautographic studies on the nucleic acid synthesis in the retina of chicken embryo I. Light microscopic radioautography. Shinshu Med J 32: 231-240.
60. Gunarso W. (1984). Radioautographic studies on the nucleic acid synthesis in the retina of chicken embryo II. Electron microscopic radioautography. Shinshu Med J 32: 241-248.
61. Gunarso W, Gao F, Cui H, Ma H, Nagata T. (1996). A light and electron microscopic radioautographic study on RNA synthesis in the retina of chick embryo. Acta Histochem 98: 300-322.
62. Gunarso W, Gao F, Nagata T. (1997). Development and DNA synthesis in the retina of chick embryo observed by light and electron microscopic radioautography. Cell Mol Biol 43: 189-201.
63. Gao F, Toriyama K, Nagata T. (1992). Light microscopic radioautographic study on the DNA synthesis of prenatal and postnatal aging mouse retina after labeled thymidine injection. Cell Mol Biol 38: 661-668.
64. Kong Y, Nagata T. (1994). Electron microscopic radioautographic study on nucleic acid synthesis of perinatal mouse retina. Med Electron Microsc 27: 366-368.
65. Cui H, Gao F, Nagata T. (2000). Light microscopic radioautographic study on protein synthesis in perinatal mice corneas. Acta Histochem Cytochem 33: 31-37.
66. Nagata T. (2001). Special Cytochemistry in Cell Biology. Internat. Rev. Cytol., Jeon KW, ed., Academic Press, New York, Vol 211: pp. 33-151.
67. Nagata T. (2002). Radioautographology General and Special, Prog Histochem Cytochem. Graumann W ed. Urban & Fischer, Jena, Vol 37 No 2: pp. 57-226.
68. Nagata T, Murata F. (1977). Electron microscopic dry-mounting radioautography for diffusible compounds by means of ultracryomicrotomy. Histochemistry 54: 75-82.
69. Nagata T, Ohno S, Murata F. (1977). Electron microscopic dry-mounting radioautography for soluble compounds. Acta Pharmacol. Toxicol. 41: 63-64.
70. Nagata T. (1994). Electron microscopic radioautographology with cryo-fixation and dry-mounting procedure. Acta Histochem Cytochem. 27: 471-489.
71. Guttes E, Guttes S. (1964). Thymidine incorporation by mitochondria in Physarum polycephalum. Science 145:1057-1058.
72. Schuster FL. (1965). A deoxyribose nucleic acid component in mitochondria of Didymium nigirpes, a slime mold. Exp Cell Res 39: 329-345.
73. Stone GE, Miller OL Jr. (1965). A stable mitochondrial DNA in Tetrahymena pyriformis. Exp Zool 159: 33-37.
74. Chévremont M. (1963). Cytoplasmic deoxyribonucleic acids: Their mitochondrial localization and synthesis in somatic cells under experimental conditions and during the normal cell cycle in relation to the preparation for mitosis. Cell Growth and Cell Division. Symposia of the Internat Soc for Cell Biol. Harris RJC ed. Academic Press, New York, Vol. 2: pp. 323-333.
75. Meyer RR. (1966). Cytochemical and electron microscopic studies of mitochondrial DNA in cultured chick fibroblasts grown at subnormal temperature. J Cell Biol 31: 151-152.
76. Schatz G. (1970). Biogenesis of mitochondria. Membranes of Mitochondria and Chloroplasts. Racker E, ed. Van Nostrand-Reinhold, New York, pp. 251-314.
77. Tandler B, Hoppel CL. (1972). Mitochondria, Ultrastructure of Cells and Organisms, Locke M ed. Academic Press, New York and London, pp. 1-59.
78. Vogel FS, Kemper L. (1967). Intrinsic ribonucleic acid synthesis in mitochondria of Agaricus campestris underscoring the probability of an extra-nuclear genetic system. Exp Cell Res 47: 209-201.
79. Gibbs SP. (1968). Autoradiographic evidence for the in situ synthesis of chloroplast and mitochondrial RNA. J Cell Sci 81: 227-228.
80. André J. (1968). Donneés récentes sur la physiologie des mitochondries. C R Soc Biol 162: 7-12.
81. André J, Marinozzi V. (1965). Présence dans les mitochondries de particules ressemblant aux ribosomes. J Microsc Paris 4: 615-626.
82. Curgy J J. (1968). Synthese d'ARN in vivo par les mitochondries du foie de poussin et du souriceau. Etude autoradiographique au microscope électronique. J Microscop 7: 849-864.
83. Nussdorfer GG, Mazzochi G. (1971). Effect of ACTH on mitochondrial RNA synthesis of rat adrenocortical cells. Z Zellforsch 118: 35-48.
84. Juang HH. 2004. Modulation of iron on mitochondrial aconitase expression in human prostatic carcinoma cells. Mol Cell Biochem 265: 185-194.
85. Mahata B, Bhattacharyya SN, Mukherjee S, Adhya S. 2005. Correction of translational defects in patient-derived mutant mitochondria by complex-mediated import of a cytoplasmic tRNA. J Biol Chem 18: 5141-5144.
86. Maneiro E, Lopez-Armada MJ, de Andres MC, Carames B, Martin MA, Bonilla A, Del Hoyo P, Galdo F, Arenas J, Blanco FJ. (2005). Effect of nitric oxide on mitochondrial respiratory activity of human articular chondrocytes. Ann Rheum Dis 64: 388-395.
87. Nagata T. (2007) Electron microscopic radioautographic study on protein synthesis in mitochondria of binucleate hepatocytes of aging mice. The Sci World J. 7: 1008-1023.
88. Nagata T. (2008). Sexual difference between the macromolecular synthesis of hepatocyte mitochondria in male and female mice in aging as revealed by electron microscopic radioautography. In, Women and Aging Research, Nova Sci., N.Y., USA, pp. -.
89. Caro LG. (1962). High resolution autoradiography II. The problem of resolution. J Cell Biol 15: 189-198
90. Salpeter MM, Bachmann L, Salpeter EE. (1969). Resolution in electron microscope radioautography. J Cell Biol 44: 1-20.
91. Nadler NJ. (1971). The interpretation of grain counts in electron microscope radioautography. J Cell Biol 49: 377-382.
92. Uchida K, Mizuhira V. (1971). Electron microscope autoradiography with special reference to the problem of resolution. Arch Histol Jap 31: 291-320.
93. Murata F, Yoshida K, Ohno S, Nagata T. (1979). Electron microscopic radioautography using a combination of phenidon developer and domestic emulsion. Acta Histochem Cytochem 12: 443-450.
94. Chi JCH, Suyama Y. (1970). Comparative studies on mitochondrial and cytoplasmic ribosomes of Tetrahymena pyriformis. J Mol Biol 53: 531-556.
95. Curgy J J. (1970). Incorporation of in vivo et in vitro d'uridine-3H dans l'ARN des mitochondries. Exp Cell Res 62: 359-367.
96. Korr H, Phillipi V, Helg C, Schiefer J, Graeber MB, Kreutzberg GW. (1997). Unscheduled DNA synthesis and mitochondrial DNA synthetic rate following injuring of the facial nerve. Acta Neuropathol 94: 557-566.
97. Korr H, Kurz C, Seidler TO, Sommer D, Schmitz C. (1998). Mitochondrial DNA synthesis studied autoradiographically in various cell types in vivo. Braz J Med Biol Res 31: 289-298.
98. Schmitz C, Axmacher B, Zunker U, Korr H. (1999). Age related changes of DNA repair and mitochondrial DNA synthesis in the mouse brain. Acta Neuropathol 97: 71-81.
99. Nagata T. 2006. Electron microscopic radioautographic study on protein synthesis in hepatocyte mitochondria of developing mice. Ann. Microsc. 6: 43-54 (2006).
100. Nagata T. Macromolecular synthesis in hepatocyte mitochondria of aging mice as revealed by electron microscopic Radioautography. I. Nucleic acid synthesis. II. Protein synthesis. Modern Research and Educational Topics in Microscopy, edited by Mendes-Vilas A. and Alvarez JD., Formatex, Badajaz, Spain, 2007, pp. 245-271.