Techniques and application of electron microscopic radioautography

Journal of Electron Microscopy

Volumn 45, Issue 4, 1996, Pages 258-274

  Nagata, Tetsuji ^a  

^a SHINSHU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

DNA; Protein; Radioautography; RNA; Synthesis

Indexed keywords

BIOSYNTHESIS; ELECTRONS; INORGANIC COMPOUNDS; MOUNTINGS; RNA;

'DRY' [; CHEMICAL FIXATION; CRYOFIXATION; GLUTARALDEHYDES; INSOLUBLE COMPOUNDS; MACROMOLECULAR SYNTHESIS; MICROSCOPIC LEVELS; OSMIUM TETROXIDE; RADIOAUTOGRAPHY; SOLUBLE COMPOUNDS;

PROTEINS;

EID: 0000150851     PISSN: 00220744     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jmicro.a023442     Document Type: Article

References (80)

1. Nagata T: Radiolabeling of soluble and insoluble compounds as demonstrated by light and electron microscopy. In: Wegmann RJ, Wegmann MA, eds. Recent advances in cellular and molecular biology, vol 6. Molecular biology of pyridines and DNA. peroxisomes, organelles and cell movements. Leuven. Belgium: Peeters Press, 9-21 (1992)
2. Nagata T, Nawa T, Vokota S: A new technique for electron microscopic dry-mounting radioautography of soluble compounds. Histochemie 18: 241-249 (1969)
3. Nagata T, Ohno S, Murata F: Electron microscopic dry-mounting radioautography for soluble compounds. Acta Pharmacol Toxicol 41: 63-64 (1977)
4. Nagata T, Shimamura K: Radioautographic studies on calcium absorption. I. Calcium absorption in the stomach of rat. Med J Shinshu Univ 3: 83-90 (1958)
5. Nagata T, Shimamura K: Radioautographic studies on calcium absorption. II. Calcium absorption in the intestines of rat. III. Distribution of radiocalcium in the liver and the kidney of rat after oral administration. Med J Shinshu Univ 4: 1-18 (1959)
6. Mizuhira V, Uchida K, Totsu J, Shindo H: Studies on the absorption of S-benzoylthiamine O-monophosphate. Vitamins (Japanese) 38: 334-346 (1968) (in Japanese)
7. Appleton TC: Autoradiography of soluble labeled compounds. J Roy Micro Soc 83: 277-281 (1964)
8. Christensen AK: Frozen thin sections of fresh tissue for electron microscopy, with a description of pancreas and liver. J Cell Biol 51: 772-804 (1971)
9. Nagata T, Murata F: Electron microscopic dry-mounting radioautography for diffusible compounds by means of ultracryotomy. Histochemistry 54: 75-82 (1977)
10. Nagata T, Nawa T: A modification of dry-mounting technique for radioautography of water-soluble compounds. Histochemic 7: 370-371 (1966)
11. Mizuhira V, Shiihashi M, Futaesaku Y: High-speed electron microscope autoradiographic studies of diffusible compounds. J Histochem Cytochem 29: 143-160 (1981)
12. Futaesaku Y, Mizuhira V: Negative-staining autoradiography: A new technique for ultracryotomy utilizing an interposed film. J Histochem Cytochem 34: 1085-1094 (1986)
13. Edwards LC, Udupa KN: Autoradiographic determination of S35 in tissues after injection of methionin-S35 and sodium sulfate-S35. J Biophys Biochem Cytol 3: 757-770 (1957)
14. Smitherman TC, Debons AF, Pittman JA, Stephens V: Movement of water-soluble material in Mayer's albumin and a simplified dry-mounting method for autoradiography. Nature 198: 499-500 (1963)
15. Miller OL Jr, Stone GE, Prescott DM: Autoradiography of soluble materials. J Cell Biol 23: 654-658 (1964)
16. Nagata T, Nawa T, A modification of dry-mounting technique for radioautography of water-soluble comounds. Histochemie 7: 370-371 (1966)
17. Stirling CE, Kinter WB: High resolution radioautography of galactose-3H accumulation in rings of hamster intestine. J Cell Biol 35: 585-604 (1967)
18. Nagata T: Radioautography data book. Tokyo: IPC Inc. 1-425 (1982)
19. Nagata T: Simple method for mass production of radioautographs. Saibo (The Cell) 14: 40-50 (1982) (in Japanese)
20. Nagata T: Principles and techniques of electron microscopic radioautography. In: World Health Organization ed. National training course on the application of electron microscopy in biomedical research and diagnosis of human diseases. Beijing: The Ministry of Public Health Peoples Republic of China. 139-157 (1990)
21. Murata F, Yoshida K, Ohno S, Nagata T: Electron microscopic radioautography using a combination of phenidone developer and domestic emulsion. Acta Histochem Cytochem 12: 443-450 (1979)
22. Nagata T: Three dimension observation of whole mount cultured cells stained with histochemical reaction by ultrahigh voltage electron microscopy. Cell Mol Biol 41: 783-792 (1995)
23. Nagata T: Quantitative analysis of histochemical reactions: Image analysis of light and election microscopic radioautograms. Acta Histochem Cytochem 26: 281-291 (1993)
24. Altmann R: Die Elementarorganismen und ihre Beziehungen zur Zellen. Leipzig: Veit u Co (1890)
25. Gersh I: The Altmann technique for fixation by drying while freezing. Anat Rec 53: 309-324 (1932)
26. Gersh I: The preparation of frozen-dried tissue for electron microscopy. J Biophys Biochem Cytol Suppl 2: 37-55 (1956)
27. Nagata T: Electron microscopic radioautography with cryofixation and dry-mounting procedure. Acta Histochem Cytochem 27: 471-489 (1994)
28. Pearse AGE: Histochemistry, theoretical and applied. 3rd ed. vol I. London: J. & A Churchill Ltd. 50-51 (1968)
29. Luft JE: Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol 9: 409-414 (1961)
30. Nagata T, Murata F, Yoshida K, Ohno S, Iwadare N: Whole mount radioautography of cultured cells as observed by high voltage electron microscopy. In: Proc 5th internat congr high voltage electron microsc. 347-350 (1977)
31. Simpson WL: An experimental analysis of the Altmann technic of freezing drying. Anat Rec 80: 173-185 (1941)
32. Lison L: Histochimic et cytochimie animales. 3rd ed. Paris: Gauthier-Villards (1960)
33. Trump BF: Autoradiography of diffusible substances. In: Roth LJ. Stumpf WE. eds. Autoradiography of diffusible substance. New york and London: Academic Press. 211-240 (1969)
34. van Harrevelt A, Crowell J: Electron microscopy after rapid freezing on a metal surface and substitution fixation. Anat Rec 149: 381-386 (1964)
35. Bernhard W, Leduc EH: Ultrathin frozen sections. I. Methods and ultrastructural preservation. J. Cell Biol 34: 757-771 (1967)
36. Tokuyasu K: A technique for ultracryotomy of cell suspensions and tissues. J. Cell Biol 57: 551-565 (1973)
37. Sakai T, Shimakura S, Sakamoto H: The ultrathin section for low temperature. In: Proc 8th internat congr electron micros. vol 2. 56-57 (1974)
38. Nagata T, Ohno S, Yoshida K, Murata F: A simple picking-up device for cryosections. Sci Tools 25: 59-60 (1978)
39. Nagata T: Electron microscopic dry-mounting autoradiography. In: Proc 4th internat congr histochem cytochem. 43-44 (1972)
40. Reynolds ES: The use of lead citrate at high pH as an electron opaque stain in electron microcopy. J Cell Biol 17: 208-212 (1963)
41. Leblond CP: The life history of cells in renewing systems. Am J Anat 160: 113-158 (1981)
42. Ma H, Nagata T: Electron microscopic radioautographic studies on DNA synthesis in the hepatocytes of aging mice as observed by image analysis. Cell Mol Biol 36: 73-84 (1990)
43. Nagata T, Shibata O, Nawa T: Incorporation of tritiated thymidine into mitochondrial DNA of the liver and kidney cells of chickens and mice in tissue culture. Histochemie 10: 305-308 (1967)
44. Nagata T, Ohno S, Yoshida K, Murata F: Nucleic acid synthesis in proliferating peroxisomes of rat liver as revealed by electron microscopical radioautography. Histochem J 14: 197-204 (1982)
45. Nagata T, Usuda N, Ma H: Electron microscopic radioautography of nucleic acid synthesis in pancreatic acinar cells of prenatal and postnatal aging mice. In: Proc XIth internat congr electron microsc, vol 2, 2281-2282 (1984)
46. Chen S, Gao F, Kotani A, Nagata T: Age-related changes of male mouse submandibular gland: A morphometric and radioautographic study. Cell Mol Biol 41: 117-124 (1995)
47. 3H-thymidine incorporation and acid phosphatase activity in mouse spleen: Electron microscopic radioautography and cytochemistry. Cell Mol Biol 38: 115-122 (1992).
48. 3H-thymidine incroporation at different stages of muscle development in aging mice. Cell Mol Biol 39: 553-560 (1993)
49. Ito M, Nataga T: Radioautographic study on DNA synthesis in adrenal glands of developing and aging mice. J Histochem Cytochem 42: 988 (1994)
50. Sun L, Gao F, Duan H, Nagata T: An electron microscopic radioautographic study on the DNA synthesis of pulmonary tissue cell in aging mice. Med Electron Microsc 28: 129-131 (1995)
51. Hanai T, Nagata T: Study on the nucleic acid synthesis in the aging mouse kidney by light and electron microscopic radioautography. In: Nagata T, ed Radioautography in medicine. Matsumoto: Shinshu Univ Press. 209-214 (1994)
52. Gao F: Study on the macromolecular synthesis in aging mouse seminiferous tubules by light and electron microscopic radioautography. Cell Mol. Biol 39: 659-672 (1993)
53. Gao F, Sun L, Chen S, Nagata T: Radioautographic study on the nucleic acid and protein synthesis in Leydig cells of aging mouse testis. In: Nagata T, ed. Radioautography in medicine, Proc 4th int symp radioautography. Matsumoto: Shinshu Univ Press. 125-129 (1994)
54. Yamada AT, Nagata T: Light and electron microscopic radioautography of DNA synthesis in the endometria of pregnant-ovaricetomized mice during activation for implantation window. Cell Mol Biol 38: 763-774 (1992)
55. Cui H: Light microscopic radioautographic study on DNA synthesis of nerve cells in the cerebella of aging mice. Cell Mol Biol 41: 1139-1154 (1995)
56. Gao F, Li S, Dua H, Ma H, Nagata T: Electron microscopic radioautography on the DNA synthesis of prenatal and postnatal mice retina after labeled thymidine injection. J Clin Electron Microsc 25: 721-723 (1992)
57. Kong Y, Usuda N, Nagata T: Radioautographic study on DNA synthesis of the retina and retinal pigment epithelium of developing mouse embryos. Cell Mol Biol 38: 263-272 (1992)
58. Kong Y: Electron microscopic radioautographic study on DNA synthesis in perinatal mouse retina. Cell Mol Biol 39: 55-64 (1993)
59. Gao F, Toriyama K, Ma H, Nagata T: Light microscopic radioautographic study on DNA synthesis in aging mice corneas. Cell Mol Biol 39: 435-441 (199)
60. Ma H, Nagata T: Study on RNA synthesis in the liver of aging mice by means of electron microscopic radioautography. Cell Mol Biol 36: 589-600 (1990)
61. Nagala T: Electron microscopic radioautography and analytical electron microscopy. J Clin Electron Microsc 24: 441-442 (1991)
62. Sun L: Age-rdated changes of RNA synthesis in the lungs of aging mice by light and electron microscopic radioautography. Cell Mol Biol 41: 1061-1072 (1995)
63. 3H-uridine labeling in vitro. Cell Mol Biol 38: 399-405 (1992)
64. Gao F: Study on the macromolecular synthesis in aging mouse seminiferous tubules by light and electron microscopic radioautography. Cell Mol Biol 39: 659-672 (1993)
65. Ma H, Nagata T: Protein synthesis in the livers of aging mice studies by electron microscopic radioautography. Cell Mol Biol 37: 607-615 (1991)
66. Jamieson JD, Palade GE: Intracellular transport of secretory proteins in the pancreatic exocrine cells. J Cell Biol 34: 577-615 (1967)
67. Nagata T, Ohno S, Murata F: Electron microscopic dry-mounting radioautography for soluble compounds. Acta Pharmacol Toxicol 41: 63-64 (1977)
68. Yamada AT: Timely and topologically defined protein synthesis in the peri-implanting mouse endometrium revealed by light and electron microscopic radioautography. Cell Mol Biol 39: 1-12 (1993)
69. 3H-proline by mouse decidual cells. Cells Mol Biol 37: 315-323 (1991)
70. Nagata T, Kawahara I, Usuda N, Maruyama M, Ma H: Radioautographic studies on the glycoconjugate synthesis in the gastrointestinal mucosa of the mouse. In: Ohyama M, Muramatsu T. eds. Glycoconjugates in medicine. Tokyo: Profes Postgrad Serv Ltd. 252-256 (1988)
71. 3H-glucasamine into the pancreatic cells of aging mice as demonstrated by electron microscopic radioautography. J Clin Electron Microsc 25: 646-647 (1992)
72. 4 in mouse ovary during the estrus cycle. J Clin Electron Microsc 25: 669-670 (1992)
73. Nagata T, Usude N, Ma H: Electron microscopic radioautography of lipid synthesis in pancreatic cells of aging mice. J Clin Electron Microsc 23: 841-842 (1990)
74. Nagata T: Quantitative analysis of histochemical reactions: Image analysis of light and electron microscopic radioautograms. Acta Histochem Cytochem 26: 281-291 (1993)
75. 3H-taurine in mouse skeletal muscle cells by light and electron microscopic radioautography. Cell Mol Biol 39: 397-404 (1993)
76. 3H-methyl prednisolone as observed by electron microscopic dry-mounting radioautography. In: Proc 9th internat congr electron microsc. 40-41 (1978)
77. 3H-befunolol by means of electron microscopic radioautography of cryo-fixed ultrathin sections. J Clin Electron Microsc 16: 737-738 (1983)
78. Momose Y, Naito J, Nagata T: Radioautographic study on the localization of an anti-allergic agent, tranilast, in the rat liver. Cell Mol Biol 35: 347-355 (1989)
79. Nagata T, Nishigaki T, Momose Y: Localization of antiallergic agent in rat mast cells demonstrated by light and electron microscopic radioautography. Acta Histochem Cytochem 19: 669-683 (1986)
80. Nagata T, Yoshida K, Murata F: Demonstration of hot and cold mercury in the human thyroid tissues by means of radioautography and chemography. Acta Phamacol Toxicol 41: 60-61 (1977)