Genotoxic fungicide methyl thiophanate as an oxidative stressor inducing 8-oxo-7,8-dihydro-2′-deoxyguanosine adducts in DNA and mutagenesis

Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes

Volumn 45, Issue 1, 2010, Pages 40-45

  Saquib, Quaiser ^a   Al Khedhairy, Abdulaziz A ^a   Singh, Braj R ^a   Arif, Jamal M ^b   Musarrat, Javed ^a  

^a KING SAUD UNIVERSITY   (Saudi Arabia)

^b INTEGRAL UNIVERSITY   (India)

Author keywords

8 oxodG; Fungicide; Methyl thiophanate; Mitochondrial membrane potential; Mutagenicity; ROS

Indexed keywords

8-OXODG; ADDUCT FORMATION; CALF THYMUS DNA; CATIONIC DYES; DOSE-DEPENDENT; FLUORESCEIN DIACETATE; FLUORESCENCE INTENSITIES; GENOTOXIC; HUMAN LYMPHOCYTES; MITOCHONDRIAL DYSFUNCTION; MITOCHONDRIAL MEMBRANE POTENTIAL; MUTAGENICITY; OXIDATIVE DNA DAMAGE; OXIDATIVE POTENTIAL; REACTIVE OXYGEN SPECIES;

BIOMOLECULES; CELL MEMBRANES; DNA; DYES; FLUORESCENCE; GENES; MAGNESIUM PRINTING PLATES; MUTAGENESIS; OXYGEN; RHODIUM; TELLURIC PROSPECTING;

FUNGICIDES;

8 OXO 7 HYDRODEOXYGUANOSINE; 8-OXO-7-HYDRODEOXYGUANOSINE; CALF THYMUS DNA; DEOXYGUANOSINE; DNA; DRUG DERIVATIVE; FUNGICIDE; MITOCHONDRIAL DNA; REACTIVE OXYGEN METABOLITE; THIOPHANATE;

BIOASSAY; CARCINOGEN; FUNGICIDE; GENOTOXICITY; MITOCHONDRIAL DNA; MUTAGENICITY; OXYGEN;

ANIMAL; ARTICLE; CATTLE; DNA DAMAGE; DNA STRAND BREAKAGE; DOSE RESPONSE; DRUG EFFECT; HUMAN; LYMPHOCYTE; METABOLISM; MUTAGENESIS; OXIDATION REDUCTION REACTION; OXIDATIVE STRESS;

ANIMALS; CATTLE; DEOXYGUANOSINE; DNA; DNA BREAKS; DNA DAMAGE; DNA, MITOCHONDRIAL; DOSE-RESPONSE RELATIONSHIP, DRUG; FUNGICIDES, INDUSTRIAL; HUMANS; LYMPHOCYTES; MUTAGENESIS; OXIDATION-REDUCTION; OXIDATIVE STRESS; REACTIVE OXYGEN SPECIES; THIOPHANATE;

EID: 77649119442     PISSN: 03601234     EISSN: 15324109     Source Type: Journal    
DOI: 10.1080/03601230903404325     Document Type: Article

References (24)

1. Bagchi, D.; Bagchi, M.; Hassoun, E.A.; Stohs, S.J. In vitro and in vivo generation of reactive oxygen species, DNA damage and lactate dehydrogenase leakage by selected pesticides. Toxicol. 1995, 104, 129-140.
2. Muniz, J.F.; McCauley, L.; Scherer, J.; Lasarev, M.; Koshy, M.; Kow, Y.W.; Nazar-Stewart, V.; Kisby, G.E. Biomarkers of oxidative stress and DNA damage in agricultural workers: a pilot study. Toxicol. App. Pharmacol. 2008, 227, 97-107.
3. Lee, C.H.; Kamijima, M.; Kim, H.; Shibata, E.; Ueyama, J.; Suzuki, T.; Takagi, K.; Saito, I.; Gotoh, M.; Hibi, H.; Naito, H.; Nakajima, T. 8-Hydroxydeoxyguanosine levels in human leukocyte and urine according to exposure to organophosphorus pesticides and paraoxonase 1 genotype. Int. Arch. Occup. Environ. Health. 2007, 80, 217-227.
4. Kale, M.; Rathore, N.; John, S.; Bhatnagar, D. Lipid peroxidative damage on pyrethroid exposure and alterations in antioxidant status in rat erythrocytes: a possible involvement of reactive oxygen species. Toxicol. Lett. 1999, 105, 197-205.
5. Prakasam, A.; Sethupathy, S.; Lalitha, S. Plasma and RBCs antioxidant status in occupational male pesticide sprayers. Clin. Chim. Acta 2001, 310, 107-112.
6. López, O.; Hernández, A.F.; Rodrigo, L.; Gil, F.; Pena, G.; Serrano, J.L.; Parrón, T.; Villanueva, E.; Pla, A. Changes in antioxidant enzymes in humans with long-term exposure to pesticides. Toxicol. Lett. 2007, 171, 146-153.
7. Boveris, A. Determination of the production of superoxide radicals and hydrogen peroxide in mitochondria. Methods Enzymol. 1984, 105, 429-435.
8. Olinski, R.; Gackowski, D.; Foksinski, M.; Rozalski, R.; Roszkowski, K.; Jaruga, P. Oxidative DNA damage: assessment of the role in carcinogenesis, atherosclerosis, and acquired immunodeficiency syndrome. Free Radic. Biol. Med. 2002, 33, 192-200.
9. Abdollahi, M.; Ranjbar, A.; Shadnia, S.; Nikfar, S.; Rezaie, A. Pesticides and oxidative stress: a review. Med. Sci. Monit. 2004, 10, RA141-147.
10. Cheng, K.C.; Cahill, D.S.; Kasai, H.; Nishimura, S.; Loeb, L.A. 8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G-T and A-C substitutions. J. Biol. Chem. 1992, 267, 166-172.
11. Floyd, R.A. The role of 8-hydroxyguanine in carcinogenesis. Carcinogenesis 1990, 11, 1447-1450.
12. Olinski, R.; Gackowski, D.; Rozalski, R.; Foksinski, M.; Bialkowski, K. Oxidative DNA damage in cancer patients: a cause or a consequence of the disease development? Mutat. Res. 2003, 531, 177-190.
13. Musarrat, J.; Arezina-Wilson, J.; Wani, A.A. Prognostic and Aetiological Relevance of 8-Hydroxyguanosine in Human Breast Carcinogenesis. Euro. J Cancer 1996, 32, 1209-1214.
14. Zawiaa, N.H.; Lahirib, D.K.; Cardozo-Pelaez, F. Epigenetics, oxidative stress, and Alzheimer disease. Free Radic. Biol. Med. 2009, 46, 1241-1249.
15. Saquib, Q.; Al-Khedhairy, A.A.; Al-Arifi, S.; Dhawan, A.; Musarrat, J. Assessment of methyl thiophanate-Cu (II) induced DNA damage in human lymphocytes. Toxicol. In vitro 2009, 23, 848-854.
16. Nakayama, T.; Kinnura, T.; Kodama, M.; Nagata, C. Generation of hydrogen peroxide and superoxide anions from active metabolites of napthylamines and amino azo dyes: possible role in carcinogenesis. Carcinogenesis 1983, 4, 765-769.
17. Jaiswal, R.; Khan, M.A.; Musarrat, J. Photosensitized paraquatinduced structural alterations and free radical mediated fragmentation of serum albumin. J. Photochem. Photobiol. B. 2002, 67, 163-170.
18. Gupta, R.C.; Arif, J.M. An improved 32P-postlabeling assay for the sensitive detection of 8-oxodeoxyguanosine in tissue. Chem. Res. Toxicol. 2001, 14, 951-957.
19. Sambrook, J.; Fritsch, E.F.; Maniatis, T. Isolation of bacteriophage and plasmid DNA. In Molecular Cloning: A Laboratory Manual, Second Edition; Sambrook, J., Fritsch, E.F., Maniatis, T., Eds.; Cold Spring Harbour Laboratory Press: New York 1989.
20. Calviello, C.; Piccionia, E.; Boninsegna, A.; Tedesco, B.; Maggiano, N.; Serini, S.; Wolf, F.I.; Palozza, P. DNA damage and apoptosis induction by the pesticide Mancozeb in rat cells: Involvement of the oxidative mechanism. Toxicol. App. Pharmacol. 2006, 211, 87-96.
21. Shah, R.G.; Lagueux, J.; Kapur, S.; Levallois, P.; Ayotte, P.; Tremblay, M.; Zee, J.; Poirier, G.G. Determination of genotoxicity of the metabolites of the pesticides Guthion, Sencor, Lorox, Reglone, Daconil and Admire by 32P-postlabeling. Mol. Cell. Biochem. 1997, 169, 177-184.
22. Le Goff, J.; Aandre, V.; Labailly, P.; Pottier, D.; Perin, F.; Perin, O.; Gauduchon, P. Seasonal variations of DNA-adduct patterns in open field farmers handling pesticides. Mutat. Res. 2005, 587, 90-102.
23. Hrelia, P.; Fimognari, C.; Vigagini, F.; Maffei, F.; Forti, G.C. A cytogenetic approach to the study of genotoxic effects of fungicides: an in vitro study in lymphocyte cultures with thiophanate methyl. ATLA 1996, 24, 597-601.
24. Fimognari, C.; Nüsse, M.; Hrelia, P. Flow cytometric analysis of genetic damage, effect on cell cycle progression, and apoptosis by Thiophanate-methyl in human lymphocytes. Environ. Mol. Mutagen. 1999, 33, 173-176.