Secreted production of self-assembling peptides in Pichia pastoris by fusion to an artificial highly hydrophilic protein

Journal of Biotechnology

Volumn 146, Issue 1-2, 2010, Pages 66-73

  Moers, Antoine P H A ^a   Wolbert, Emil J H ^a   de Wolf, Frits A ^a   Werten, Marc W T ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Enterokinase; Fusion protein; Pichia pastoris; Protein secretion; Self assembling peptide; Self supporting gel

Indexed keywords

BROAD APPLICATION; C-TERMINUS; CHEMICAL SYNTHESIS; FUSION PROTEINS; HYDROPHILIC PROTEIN; IN-VITRO; METHYLOTROPHIC YEASTS; PICHIA PASTORIS; PRODUCT DETECTION; PROTEIN SECRETION; SCALE-UP; SELF-ASSEMBLING PEPTIDES; SIMPLE SALTS;

AMINO ACIDS; GELS; HYDROPHILICITY; PRECIPITATION (CHEMICAL); SELF ASSEMBLY; SYNTHESIS (CHEMICAL);

PEPTIDES;

ACETIC ANHYDRIDE GLUTAMINYLGLUTAMINYLARGINYLPHENYLGLUTAMINYLTRYPTOPHYLGLUTAMYLGLUTAMIC ACID AMINE; ACETIC ANHYDRIDE GLUTAMINYLGLUTAMINYLORNITHYLPHENYLORNITHYLTRYTOPHYLORNITHYLPHENYLORNITHYLGLUTAMINYL GLUTAMIC ACID AMINE; CARRIER PROTEIN; ENTEROPEPTIDASE; HYBRID PROTEIN; LYSINE; NANOMATERIAL; OLIGOPEPTIDE; ORNITHINE; PHOSPHORUS DERIVATIVE; SODIUM CHLORIDE; SUTURE MATERIAL; UNCLASSIFIED DRUG;

AMINO ACID ANALYSIS; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENCAPSULATION; ENZYME DEGRADATION; HYDROGEL; HYDROPHILICITY; IN VITRO STUDY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHYLOTROPHIC YEAST; NONHUMAN; PEPTIDE SYNTHESIS; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN CLEAVAGE; PROTEIN DOMAIN; PROTEIN SECRETION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TISSUE ENGINEERING;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENTEROPEPTIDASE; HYDROPHOBICITY; LEAST-SQUARES ANALYSIS; PEPTIDES; PICHIA; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

PICHIA PASTORIS;

EID: 77649115008     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.01.010     Document Type: Article

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