Surface extenders and an optimal pore size promote high dynamic binding capacities of antibodies on cation exchange resins

Journal of Chromatography A

Volumn 1216, Issue 20, 2009, Pages 4372-4376

  Hart, David S ^a   Harinarayan, Chithkala ^a   Malmquist, Gunnar ^b   Axén, Andreas ^b   Sharma, Mandakini ^a   van Reis, Robert ^a  

^a GENENTECH INC   (United States)

^b GE Healthcare Bio Sciences AB   (Sweden)

Author keywords

Antibody; Critical ionic strength; Dynamic binding capacity; Exclusion; Ligand density; Pore size; Surface extenders

Indexed keywords

CATION EXCHANGE RESINS; CHROMATOGRAPHIC RESINS; CRITICAL IONIC STRENGTH; DEXTRAN SURFACES; DISTRIBUTION COEFFICIENTS; DYNAMIC BINDING CAPACITY; EXCLUSION; HIGH CAPACITIES; HIGH DYNAMICS; LIGAND DENSITY; MANUFACTURING FACILITIES; RECOMBINANT PROTEIN EXPRESSIONS; THERAPEUTIC ANTIBODIES;

DEXTRAN; FUNCTIONAL POLYMERS; GLUCOSE; ION EXCHANGE RESINS; IONIC STRENGTH; LIGANDS; OPTIMIZATION; PORE SIZE; POSITIVE IONS;

ANTIBODIES;

ANTIBODY; CATION EXCHANGE RESIN; DEXTRAN; LIGAND;

ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CONCENTRATION (PARAMETERS); CONDUCTANCE; DENSITY; DYNAMIC BINDING CAPACITY; IONIC STRENGTH; PARTICLE SIZE; PRIORITY JOURNAL; SURFACE PROPERTY;

ANTIBODIES, MONOCLONAL; CATION EXCHANGE RESINS; CHROMATOGRAPHY, ION EXCHANGE; DEXTRANS; OSMOLAR CONCENTRATION; PARTICLE SIZE; POROSITY; PROTEIN BINDING;

EID: 64649098796     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2008.11.083     Document Type: Article

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