Phenazopyridine induces and synchronizes neuronal differentiation of embryonic stem cells

Journal of Cellular and Molecular Medicine

Volumn 13, Issue 9 B, 2009, Pages 3517-3527

  Suter, David M ^a   Preynat Seauve, Olivier ^a   Tirefort, Diderik ^a   Feki, Anis ^b   Krause, Karl Heinz ^a  

^a UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

^b GENEVA UNIVERSITY HOSPITALS   (Switzerland)

Author keywords

Embryonic stem cells neuronal differentiation phenazopyridine; Main topics: neuronal differentiation of embryonic stem cells; Small molecule driven differentiation

Indexed keywords

PHENAZOPYRIDINE;

ANIMAL; ARTICLE; CELL DIFFERENTIATION; CELL LINEAGE; COCULTURE; CULTURE TECHNIQUE; CYTOLOGY; EMBRYONIC STEM CELL; FLUORESCENCE MICROSCOPY; HUMAN; METABOLISM; METHODOLOGY; MOUSE; NERVE CELL;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINEAGE; COCULTURE TECHNIQUES; EMBRYONIC STEM CELLS; HUMANS; MICE; MICROSCOPY, FLUORESCENCE; NEURONS; PHENAZOPYRIDINE;

EID: 77449145319     PISSN: 15821838     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1582-4934.2009.00660.x     Document Type: Article

References (22)

1. Pruszak J, Sonntag KC, Aung MH. Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations. Stem Cells. 2007, 25:2257-68.
2. Suter D, Krause KH. Neural commitment of embryonic stem cells: molecules, pathways and potential for cell therapy. J Pathol. 2008, 215:355-68.
3. Chung S, Sonntag KC, Andersson T. Genetic engineering of mouse embryonic stem cells by Nurr1 enhances differentiation and maturation into dopaminergic neurons. Eur J Neurosci. 2002, 16:1829-38.
4. Zhao S, Nichols J, Smith AG. SoxB transcription factors specify neuroectodermal lineage choice in ES cells. Mol Cell Neurosci. 2004, 27:332-42.
5. Krosl J, Austin P, Beslu N. In vitro expansion of hematopoietic stem cells by recombinant TAT-HOXB4 protein. Nat Med. 2003, 9:1428-32.
6. Ding S, Wu TY, Brinker A. Synthetic small molecules that control stem cell fate. Proc Natl Acad Sci USA. 2003, 100:7632-7.
7. Chen S, Zhang Q, Wu X. Dedifferentiation of lineage-committed cells by a small molecule. J Am Chem Soc. 2004, 126:410-1.
8. Chen S, Do JT, Zhang Q. Self-renewal of embryonic stem cells by a small molecule. Proc Natl Acad Sci USA. 2006, 103:17266-71.
9. Sachinidis A, Schwengberg S, Hippler-Altenburg R. Identification of small signalling molecules promoting cardiac-specific differentiation of mouse embryonic stem cells. Cell Physiol Biochem. 2006, 18:303-14.
10. Campbell RE, Tour O, Palmer AE. A monomeric red fluorescent protein. Proc Natl Acad Sci USA. 2002, 99:7877-82.
11. Suter DM, Cartier L, Bettiol E. Rapid generation of stable transgenic embryonic stem cell lines using modular lentivectors. Stem Cells. 2006, 24:615-23.
12. Itoh K, Tezuka H, Sakoda H. Reproducible establishment of hemopoietic supportive stromal cell lines from murine bone marrow. Exp Hematol. 1989, 17:145-53.
13. Suter DM, Montet X, Tirefort D. A tetracycline-inducible lentivector system based on EF1-alpha promoter and native tetracycline repressor allows in vivo gene induction in implanted ES cells. J Stem Cells. 2007, 2:62-73.
14. Nat R, Nilbratt M, Narkilahti S. Neurogenic neuroepithelial and radial glial cells generated from six human embryonic stem cell lines in serum-free suspension and adherent cultures. Glia. 2007, 55:385-99.
15. Preynat-Seauve O, Suter DM, Tirefort D. Development of human nervous tissue upon differentiation of embryonic stem cells in three dimensional culture. Stem Cells. 2009, 27:509-20.
16. Barberi T, Klivenyi P, Calingasan NY. Neural subtype specification of fertilization and nuclear transfer embryonic stem cells and application in parkinsonian mice. Nat Biotechnol. 2003, 21:1200-7.
17. O'Connor MD, Kardel MD, Iosfina I. Alkaline phosphatase-positive colony formation is a sensitive, specific and quantitative indicator of undifferentiated human embryonic stem cells. Stem Cells. 2008, 26:1109-16.
18. Chen S, Takanashi S, Zhang Q. Reversine increases the plasticity of lineage-committed mammalian cells. Proc Natl Acad Sci USA. 2007, 104:10482-7.
19. Chen S, Hilcove S, Ding S. Exploring stem cell biology with small molecules. Mol Biosyst. 2006, 2:18-24.
20. Desbordes SC, Placantonakis DG, Ciro A. High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells. Cell Stem Cell. 2008, 2:602-12.
21. Gaines KK. Phenazopyridine hydrochloride: the use and abuse of an old standby for UTI. Urol Nurs. 2004, 24:207-9.
22. Sarichelou I, Cappuccio I, Ferranti F. Metabotropic glutamate receptors regulate differentiation of embryonic stem cells into GABAergic neurons. Cell Death Differ. 2008, 15:700-7.