A tetracycline-inducible lentivector system based on EF1-α promoter and native tetracycline repressor allows in vivo gene induction in implanted ES cells

Journal of Stem Cells

Volumn 2, Issue 2, 2007, Pages 63-72

  Suter, David M ^a   Montet, Xavier ^a   Tirefort, Diderik ^a   Cartier, Laetitia ^a   Krause, Karl Heinz ^a  

^a UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

DOXYCYCLINE; ELONGATION FACTOR 1ALPHA; LENTIVIRUS VECTOR; TETRACYCLINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL LINE; CONCENTRATION RESPONSE; CONTROLLED STUDY; EMBRYO; EMBRYONIC STEM CELL; ENZYME REPRESSION; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; GENE INDUCTION; GENE SWITCHING; HUMAN; HUMAN CELL; IN VIVO GENE TRANSFER; MOUSE; NERVE CELL DIFFERENTIATION; NONHUMAN; PROMOTER REGION; REPRESSOR GENE; STEM CELL TRANSPLANTATION; TRANSGENE;

MUS;

EID: 49449109903     PISSN: 15568539     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Gossen M, Bujard H. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA 1992; 89: 5547-51.
2. Shockett P, Difilippantonio K Hellman N et al. A modified tetracycline-regulated system provides autoregulatory, inducible gene expression in cultured cells and transgenic mice. Proc. Natl. Acad. Sci. USA 1995; 92: 6522-6.
3. Gossen M, Freundlieb S, Bender G et al. Transcriptional activation by tetracyclines in mammalian cells. Science 1995; 268: 1766-9.
4. Szulc J, Wiznerowicz M, Sauvain MO et al. A versatile tool for conditional gene expression and knockdown. Nat. Methods 2006; 3: 109-16.
5. Yao F, Svensjo T, Winkler T et al. Tetracycline repressor, tetR, rather than the tetR-manimalian cell transcription factor fusion derivatives, regulates inducible gene expression in mammalian cells. Hum. Gene Ther. 1998; 9: 1939-50.
6. Gallia GL, Khalili K. Evaluation of an autoregulatory tetracycline regulated system. Oncogene 1998; 16: 1879-84
7. Baron U, Gossen M Bujard H. Tetracycline-controlled transcription in eukaryotes: novel umsactivators with graded transactivation potential. Nucleic Acids Res. 1997; 25: 2723-9.
8. Bryja V, Pachernik J, Kubala L et al. ne reverse tetracycline-controlled transactivator rtTA2s-S2 is toxic in mouse embryonic stem cells. Reprod. Nutr. Dev. 2003; 43: 477-86.
9. Xu ZL, Mizuguchi H, Mayurni T et al. Regulated gene expression from adenovirus vectors: a systematic comparison of, various inducible systems. Gene 2003; 309:145-51.
10. Toniatti C, Bujard K Cortese R et al. Gene therapy progress and prospects: transcription regulatory systems. Gene Ther. 2004; 11: 649-57.
11. Zeng X, Chen J, Sanchez JF et al. Stable expression of hrGFP by mouse embryonic stem cells: promoter activity in the undifferentiated state and during dopaminergic neural differentiation. Stem Cells 2003; 21: 647-53.
12. Suter DM, Cartier L, Bettiol E et al. Rapid generation of stable transgenic embryonic stem cell lines using modular lentivectors. Stem Cells 2006; 24: 615-23.
13. Itoh K, Tezuka H, Sakoda H et al. Reproducible establishment of hemopoietic supportive stromal cell lines from murine bone marrow. Exp. Hematol. 1989; 17:145-53.
14. Li J, Puceat M, Perez-Terzic C et al. Calreticulin reveals a critical Ca(2+) checkpoint in cardiac myofibrillogenesis. J Cell Biol. 2002; 158: 103-13.
15. Dull T, Zufferey R, Kelly M et al. A third-generation lentivirus vector with a conditional packaging system. J Virol. 1998; 72: 8463-71.
16. Campbell RE, Tour O, Palmer AE et al. A monomeric red fluorescent protein. Proc. Nad. Acad. Sci. USA 2002; 99: 7877-82.
17. Zhou BY, Ye Z, Chen G et al. Inducible and Reversible Transgene Expression in Human Stem Cells after Efficient and Stable Gene Transfer. Stem Cells 2006.
18. Adachi K, Kawase E, Yasuchika K et al. Establishment of the gene-inducible system in primate embryonic stem cell lines. Stem Cells 2006; 24: 2566-72.
19. Latta-Mahieu M, Rolland M, Caillet C et al. Gene transfer of a chimeric trans-activator is immunogenic and results in short-lived transgene expression. Hum. Gene Ther. 2002; 13: 1611-20.