Expression of human α1-proteinase inhibitor in Aspergillus niger

Microbial Cell Factories

Volumn 6, Issue , 2007, Pages

  Karnaukhova, Elena ^a   Ophir, Yakir ^a   Trinh, Loc ^b   Dalal, Nimish ^b   Punt, Peter J ^c   Golding, Basil ^a   Shiloach, Joseph ^b  

^a CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

^b NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^c TNO   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA 1 ANTITRYPSIN; GLUCAN 1,4 ALPHA GLUCOSIDASE; GLUCAN 1,4 ALPHA GLUCOSIDASE 2; RECOMBINANT ALPHA 1 ANTITRYPSIN; TRYPSIN INHIBITOR; UNCLASSIFIED DRUG;

ARTICLE; ASPERGILLUS NIGER; BIOTECHNOLOGICAL PRODUCTION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GLYCOSYLATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; WESTERN BLOTTING;

ASPERGILLUS NIGER; FUNGI;

EID: 38049159792     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-6-34     Document Type: Article

References (35)

1. Devlin GL Bottomley SP: A protein family under "stress" - serpin stability, folding and misfolding. Front Biosc 2005, 10:288-299. 10.2741/1528
2. Lomas DA: Molecular mousetraps, α1-antitrypsin deficiency and the serpinopathies. Clin Med 2005, 5:249-257. 16011217
3. Huntington JA: Shape-shifting serpins - advantages of a mobile mechanism. Trebds Biochem Sc 2005, 31:427-435. 10.1016/j.tibs.2006.06.005
4. Whisstock JC BotSomley SP: Molecular gymnastics: serpin structure, folding and misfolding. Curr Opin Struct Biol 2006, 16:761-768. 10.1016/ j.sbi.2006.10.005 17079131
5. Brantly ML Wittes JT Vogelmeier CF Hubbard RC Fells GA Crystal RG: Use of highly purified alpha 1-antitrypsin standard to establish ranges for the common normal and deficient alpha 1-antitrypsin phenotypes. Chest 1991, 100:703-708. 1889260 10.1378/chest.100.3.703
6. Crystal RG: The alpha 1-antitrypsin gene and its deficiency states. Trends Genet 1989, 5:411-417. 10.1016/0168-9525(89)90200-X 2696185
7. Crystal R: Alpha 1-Antitrypsin Deficiency. Marcel Dekker, Inc R. Crystal 1996
8. Karnaukhova E Ophir Y Golding B: Recombinant human alpha-1-proteinase inhibitor towards therapeutic use. Amino Acids 2006, 30:317-332. 10.1007/ s00726-005-0324-4 16773239
9. Maras M van Die I Contreras R van den Hondel CAMJJ: Filamentous fungi as production organisms for glycoproteins of bio-medical interest. Glycoconj J 1999, 16:99-107. 10.1023/A:1026436424881 10612410
10. Gerngross TU: Advances in the production of human therapeutic proteins yeasts and filamentous fungi. Nature Biotechnol 2004, 22:1409-1414. 10.1038/nbt1028
11. Nevalainen KMH Te'o VS Bergquist PL: Heterologous protein expression in filamentous fungi. Trends Biotechnol 2005, 23:468-474. 15967521
12. Punt PJ van Biezen N Conesa A Albers A Mangnus J van den Hondel CAMJJ: Filamentous fungi as cell factories for heterologous protein production. Trends Biotechnol 2002, 20:200-206. 10.1016/S0167-7799(02)01933-9 11943375
13. Ward M Lin C Victoria DC Fox BP Fox JA Wong DL Meerman HJ Pucci JP Fong RB Heng MH Tsurushita N Gieswein C Park M Wang H: Characterization of humanized antibodies secreted by Aspergillus niger. Appl Environ Microbiol 2004, 70:2567-2576. 404402 15128505 10.1128/ AEM.70.5.2567-2576.2004
14. Ward M Wilson LJ Kodama KH Rey MW Berka RM: Improved production of chymosin in Aspergillus by expression as a glucoamylase-chymosin fusion. Bio/Technology 1990, 8:435-440. 10.1038/nbt0590-435
15. Ward M Wilson LJ Kodama KH: Use of Aspergillus overproducing mutants, cured of integrated plasmid, to overproduce heterologous proteins. Appl Microbiol Biotechnol 1993, 39:738-743. 10.1007/BF00164459 7764120
16. Mikosch T Klemm P Gassen HG van den Hondel CAMJJ Kemme M: Secretion of active human mucus proteinase inhibitor by Aspergillus niger after KEX2-like processing of a glucoamylase-inhibitor fusion protein. J Biotechnol 1996, 52:97-106. 10.1016/S0168-1656(96)01634-3 9084209
17. Contreras R Carrez D Kinghorn JR van den Hondel CAMJJ Fiers W: Efficient KEX2-like processing of a glucoamylase-interleukin-6 fusion protein by Aspergillus nidulans and secretion of mature interleukin-6. Biotechnology (NY) 1991, 9:378-381. 10.1038/nbt0491-378
18. Archer DB Jeenes DJ MacKenzie DA: Strategies for improving heterologous protein production from filamentous fungi. Antonie Van Leeuwen 1994, 65:245-250. 10.1007/BF00871952
19. Gouka RJ Punt PJ van den Hondel CAMJJ: Efficient production of secreted proteins by Aspergillus: Progress, limitations and prospects. Appl Microbiol Biotechnol 1997, 47:1-11. 10.1007/s002530050880 9035405
20. MacKenzie DA Kraunsoe JAE Chesshyre JA Lowe G Komiyama T Fuller RS Archer DB: Aberrant processing of wild-type and mutant bovine pancreatic trypsin inhibitor secreted by Aspergillus niger. J Biotechnol 1998, 63:137-146. 10.1016/S0168-1656(98)00081-9 9772752
21. Karnaukhova E Golding B Ophir Y: Development and evaluation of an ELISA for quantification of human α1-proteinase inhibitor in complex biological mixtures. Biologicals 2007, 35:285-95. 10.1016/ j.biologicals.2006.11.002 17257853
22. Karnaukhova E Schey K Crouch RK: Circular dichroism and cross-linking studies of bacteriorhodopsin mutants. Amino Acids 2006, 30:17-23. 10.1007/s00726-005-0255-0 16477391
23. Mega T Lujan E Yoshida A: Studies on the oligosaccharide chains of human alpha 1-protease inhibitor: II. Structure of oligosaccharides. J Biol Chem 1980, 255:4057-4061. 6966283
24. Travis J Salvesen GS: Human plasma proteinase inhibitors. Annu Rev Biochem 1983, 52:655-709. 10.1146/annurev.bi.52.070183.003255 6193754
25. Kolarich D Weber A Turecek PL Schwarz HP Altmann F: Comprehensive glyco-proteomic analysis of human α1-antitrypsin and its charge isoforms. Proteomics 2006, 6:3369-3380. 10.1002/pmic.200500751 16622833
26. Kolarich D Turecek PL Weber A Mitterer A Graninger M Matthiessen P Nicolaes GAF Altmann F Schwarz HP: Biochemical, molecular characterization, and glycoproteomic analyses of α1-proteinase inhibitor products used for replacement therapy. Transfusion 2006, 46:1959-1977. 10.1111/j.1537-2995.2006.01004.x 17076852
27. Karnaukhova E Ophir Y Golding B Shrake A: Recombinant human alpha-1-proteinase inhibitor: Glycosylation, stability and biological activity. Abstracts of 10th FDA Science Forum, B-18 Washington DC 47 May 18-19, 2004
28. Roberts IN Jeenes DJ MacKenzie DA Wilkinson AP Sumner IG Archer DB: Heterologous gene expression in Aspergillus niger: A glucoamylase-porcine pancreatic prophospholipase A2 fusion protein is secreted and processed to yield mature enzyme. Gene 1992, 122:155-61. 10.1016/0378-1119(92)90043-O 1452022
29. Chill L Loc B Karnaukhova E Ophir Y Golding B Shiloach J: Production of human α1-proteinase inhibitor from Aspergillus niger 4th Recombinant Protein Production Meeting, a Comparative View on Host Physiology. Barcelona, September 21-23, 2006. Microb Cell Factories 2006, 5 (Suppl 1):P62.
30. Edelhoch H: Spectroscopic determination of tryptophan and tyrosine in proteins. Biochemistry 1967, 6:1948-1954. 10.1021/bi00859a010 6049437
31. Gordon CL Khalaj V Ram AFJ Archer DB Brookman JL Trinci APJ Jeenes DJ Doonan JH Wells B Punt PJ van den Hondel CAMJJ Robson GD: Glucoamylase::Green fluorescent protein fusion to monitor protein secretion in Aspergillus niger. Microbiol 2000, 146:415-426.
32. Sambrook J Fitsch EF Maniatis T: Molecular Cloning: A Laboratory Manual Cold Spring. Harbor Laboratory Press 1989
33. Punt PJ van den Hondel CAMJJ: Transformation of filamentous fungi based on hygromycin B and phleomycin resistance markers. Methods Enzymol 1992, 216:447-457. 1479914
34. Chase T Shaw E p-Nitrophenyl-p'-guanidino benzoate HCI: A new active site titrant for trypsin. Biochem Biophys Res Commun 1967, 29:508-514. 10.1016/0006-291X(67)90513-X 16496527
35. Tarentino AL Gomez CM Plummer TH Jr: Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase. F Biochemistry 1985, 24:4665-4671. 10.1021/bi00338a028 4063349