Thermosensitive chitosan-gelatin-glycerol phosphate hydrogels as a cell carrier for nucleus pulposus regeneration: An in vitro study

Tissue Engineering - Part A

Volumn 16, Issue 2, 2010, Pages 695-703

  Cheng, Yung Hsin ^a   Yang, Shu Hua ^b   Su, Wen Yu ^a   Chen, Yu Chun ^a   Yang, Kai Chiang ^a   Cheng, Winston Teng Kuei ^c,d   Wu, Shinn Chih ^c   Lin, Feng Huei ^a,e  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY HOSPITAL   (Taiwan)

^c NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^d TUNGHAI UNIVERSITY   (Taiwan)

^e NATIONAL HEALTH RESEARCH INSTITUTES   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOACTIVITY; BIOCOMPATIBILITY; CELL CULTURE; CELL PROLIFERATION; COAGULATION; COLLAGEN; GELATION; GELS; GENE EXPRESSION; GLYCEROL; IMPLANTS (SURGICAL); MONOLAYERS; TISSUE; TISSUE ENGINEERING;

BODY TEMPERATURE; CELL ACTIVITY; CELL CARRIER; CELL ENCAPSULATIONS; CELL VIABILITY; DISODIUM SALT; EXTRACELLULAR MATRICES; GEL STRENGTHS; GELATIN MOLECULES; GELATION TEMPERATURE; GELATION TIME; GLYCEROL PHOSPHATE; IN-VITRO; INJECTABLE HYDROGELS; INTERVERTEBRAL DISCS; MONOLAYER CULTURE; NEUTRAL PH; NEW ZEALAND WHITE RABBIT; NUCLEUS PULPOSUS; SOL/GEL TRANSITION; SULFATED GLYCOSAMINOGLYCANS; THERMO SENSITIVE; TYPE I COLLAGEN; TYPE II COLLAGENS;

HYDROGELS;

ORYCTOLAGUS CUNICULUS;

A73025; BETA-GLYCEROPHOSPHORIC ACID; CHITOSAN; DNA; DRUG CARRIER; GELATIN; GLYCEROL 2 PHOSPHATE; GLYCEROPHOSPHATE; GLYCOSAMINOGLYCAN;

ANIMAL; ARTICLE; CELL CULTURE; CELL DEATH; CYTOLOGY; DRUG EFFECT; FLOW KINETICS; GENE EXPRESSION REGULATION; HYDROGEL; INTERVERTEBRAL DISK; METABOLISM; RABBIT; REGENERATION; TEMPERATURE; TIME; YOUNG MODULUS;

ANIMALS; CELL DEATH; CELLS, CULTURED; CHITOSAN; DNA; DRUG CARRIERS; ELASTIC MODULUS; GELATIN; GENE EXPRESSION REGULATION; GLYCEROPHOSPHATES; GLYCOSAMINOGLYCANS; HYDROGEL; INTERVERTEBRAL DISK; RABBITS; REGENERATION; RHEOLOGY; TEMPERATURE; TIME FACTORS;

EID: 77049084812     PISSN: 19373341     EISSN: 1937335X     Source Type: Journal    
DOI: 10.1089/ten.tea.2009.0229     Document Type: Conference Paper

References (27)

1. Humzah, M.D., and Soames, R.W. Human intervertebral disc: structure and function. Anat Rec 220, 337, 1988.
2. Roughley, P.J. Biology of intervertebral disc aging and degeneration: involvement of the extracellular matrix. Spine 29, 2691, 2004.
3. Urban, J.P., Smith, S., and Fairbank, J.C. Nutrition of the intervertebral disc. Spine 29, 2700, 2004.
4. Roberts, S., Caterson, B., Menage, J., Evans, E.H., Jaffray, D.C., and Eisenstein, S.M. Matrix metalloproteinases and aggrecanase: their role in disorders of the human interver-tebral disc. Spine 25, 3005, 2000.
5. Nerlich, A.G., Schleicher, E.D., and Boos, N. Immuno-histologic markers for age-related changes of human lumbar intervertebral discs. Spine 22, 2781, 1997.
6. Urban, J.P., and Roberts, S. Review degeneration of the in-tervertebral disc. Arthritis Res Ther 5, 120, 2003.
7. An, H., Boden, S.D., Kang, J., Sandhu, H.S., Abdu, W., and Weinstein, J. Summary statement: emerging technique for treatment of degenerative lumbar disc disease. Spine 28, 24, 2003.
8. Ganey, T.M., and Meisel, H.J. A potential role for cell-based therapeutics in the treatment of intervertebral disc hernia-tion. Eur Spine J 11, 206, 2002.
9. Anderson, D.G., Albert, T.J., Fraser, J.K., Risbud, M., Wuis-man, P., Meisel, H.J., Tannoury, C., Shapiro, I., and Vaccaro, A.R. Cellular therapy for disc degeneration. Spine 30, 14, 2005.
10. Chiba, K., Andersson, G.B., Masuda, K., and Thonar, E.J. Metabolism of the extracellular matrix formed by interver-tebral disc cells cultured in alginate. Spine 22, 2885, 1997.
11. Baer, A.E., Wang, J.Y., Kraus, V.B., and Setton, L.A. Collagen gene expression and mechanical properties of intervertebral disc cell-alginate cultures. J Orthop Res 19, 2, 2001.
12. Sakai, D., Mochida, J., Yamamoto, Y., Nomura, T., Okuma, M., Nishimura, K., Nakai, T., Ando, K., and Hotta, T. Transplantation of mesenchymal stem cells embedded in atelo-collagen gel to the intervertebral disc: a potential therapeutic model for disc degeneration. Biomaterials 24, 3531, 2003.
13. Crevensten, G., Walsh, A.J., Ananthakrishnan, D., Page, P., Wahba, G.M., Lotz, J.C., and Berven, S. Intervertebral disc cell therapy for regeneration: mesenchymal stem cell implantation in rat intervertebral discs. Ann Biomed Eng 32, 430, 2004.
14. Roughley, P., Hoemann, C., DesRosiers, E., Mwale, F., An-toniou, J., and Alini, M. The potential of chitosan-based gels containing intervertebral disc cells for nucleus pulposus supplementation. Biomaterials 27, 388, 2006.
15. Richardson, S.M., Hughes, N., Hunt, J.A., Freemont, A.J., and Hoyland, J.A. Human mesenchymal stem cell differentiation to NP-like cells in chitosan-glycerophosphate hy-drogels. Biomaterials 29, 85, 2008.
16. Drury, J.L., and Mooney, D.J. Hydrogels for tissue engineering: scaffold design variables and applications. Bioma-terials 24, 4337, 2003.
17. Ruel-Gariépy, E., and Leroux, J.C. In situ-formation hydrogels-review of temperature-sensitive systems. Eur J Pharm Bio-pharm 58, 409, 2004.
18. Khor, E., and Lim, L.Y. Implantable applications of chitin and chitosan. Biomaterials 13, 2339, 2003.
19. Di Martino, A., Sittinger, M., and Risbud, M.V. Chitosan: a versatile biopolymer for orthopaedic tissue-engineering. Biomaterials 26, 5983, 2005.
20. Chenite, A., Chaput, C., Wang, D., Combes, C., Buschmann, M.D., Hoemann, C.D., Leroux, J.C., Atkinson, B.L., Binette, F., and Selmani, A. Novel injectable neutral solutions of chitosan form biodegradable gels in situ. Biomaterials 21, 2155, 2000.
21. Chenite, A., Buschmann, M., Wang, D., Chaput, C., and Kandani, N. Rheological characterisation of thermogelling chitosan=glycerol-phosphate solution. Carbohydr Polymers 46, 39, 2001.
22. Berger, J., Reist, M., Chenite, A., Felt-Baeyens, O., Mayer, J.M., and Gurny, R. Pseudo-thermosetting chitosan hydrogels for biomedical application. Int J Pharm 288, 197, 2005.
23. Huang, Y., Onyeri, S., Siewe, M., Moshfeghian, A., and Madihally, S.V. In vitro characterization of chitosan-gelatin scaffolds for tissue engineering. Biomaterials 26, 7616, 2005.
24. Vandervoort, J., and Ludwig, A. Preparation and evaluation of drug-loaded gelatin nanoparticles for topical ophthalmic use. Eur J Pharm Biopharm 57, 251, 2004.
25. Cho, J., Heuzey, M.C., Bégin, A., and Carreau, P.J. Physical gelation of chitosan in the presence of b-glycerophosphate: the effect of temperature. Biomacromolecules 6, 3267, 2005.
26. Bhattarai, N., Ramay, H.R., Gunn, J., Matsen, F.A., and Zhang, M. PEG-grafted chitosan as an injectable thermo-sensitive hydrogel for sustained protein release. J Control Release 103, 609, 2005.
27. Molinaro, G., Leroux, J.C., Damas, J., and Adam, A. Bio-compatibility of thermosensitive chitosan-based hydrogels: an in vivo experimental approach to injectable biomaterials. Biomaterials 23, 2717, 2002.