Metabolism of the extracellular matrix formed by intervertebral disc cells cultured in alginate

Spine

Volumn 22, Issue 24, 1997, Pages 2885-2893

  Chiba, Kazuhiro ^a   Andersson, Gunnar B J ^a   Masuda, Koichi ^a   Thonar, Eugene J M A ^a  

^a RUSH MEDICAL COLLEGE   (United States)

Author keywords

Alginate; Anulus fibrosus; Extracellular matrix; Intervertebral disc; Metabolism; Nucleus pulposus

Indexed keywords

ALGINIC ACID; CHELATING AGENT; COLLAGEN; HYALURONIC ACID; KERATAN SULFATE; PROTEOGLYCAN; PYRIDINIUM DERIVATIVE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL METABOLISM; EXTRACELLULAR MATRIX; HISTOLOGY; INTERVERTEBRAL DISK; INTERVERTEBRAL DISK DEGENERATION; NONHUMAN; NUCLEUS PULPOSUS; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN CROSS LINKING; RABBIT;

ALGINATES; ANIMALS; CELL DIVISION; CELLS, CULTURED; CHROMATOGRAPHY, GEL; COLLAGEN; CULTURE MEDIA; EXTRACELLULAR MATRIX; GLUCURONIC ACID; HEXURONIC ACIDS; INTERVERTEBRAL DISK; MICROSPHERES; PROTEOGLYCANS; PYRIDINIUM COMPOUNDS; RABBITS;

EID: 0031411017     PISSN: 03622436     EISSN: None     Source Type: Journal    
DOI: 10.1097/00007632-199712150-00011     Document Type: Article

References (41)

1. Aydelotte MB, Kuettner KE. Differences between subpopulations of cultured bovine articular chondrocytes. I. Morphology and cartilage matrix production. Connect Tissue Res 1988;18:205-22.
2. Bayliss MT, Johnstone B, O'Brien JP. 1988 Proteoglycan synthesis in the human intervertebral disc. Variation with age, region and pathology. Spine 1988;13:972-81.
3. Buckwalter JA. Spine update. Aging and degeneration of the human intervertebral disc. Spine 1995;20:1307-14.
4. Caterson B, Christner JE, Baker JR, Couchman JR. Production and characterization of monoclonal antibodies directed against connective tissue proteoglycan. Fed Proc 1985; 44:386-93.
5. Cats-Baril WL, Frymoyer JW. Identifying patients at risk of becoming disabled because of low-back pain. The Vermont Rehabilitation Engineering Center predictive model. Spine 1991;16:605-7.
6. Chandrasekhar S, Esterman MA, Hoffman HA. Microdetermination of proteoglycans and glycosaminoglycans in the presence of guanidine hydrochloride. Anal Biochem 1987;161: 103-8.
7. Chelberg MK, Banks GM, Geiger DF, Oegema TJ. Identification of heterogeneous cell populations in normal human intervertebral disc. J Anat 1995;186:43-53.
8. Dunphy MJ, Bhide MV, Smith DJ. Determination of hydroxyproline in tissue collagen hydrolysate by derivatization and isocratic reverse-phase high-performance liquid chromatography. J Chromatogr 1987;420:394-7.
9. Eyre DR. Biochemistry of the intervertebral disc. Int Rev Connect Tissue Res 1979;8:227-91.
10. Eyre DR, Koob TJ, Van Ness KP. Quantification of hydroxypyridinium crosslinks in collagen by high-performance liquid chromatography. Anal Biochem 1984;137:380-8.
11. Guo J, Jourdian GW, MacCallum DK. Culture and growth characteristics of chondrocytes encapsulated in alginate beads. Connect Tissue Res 1989;19:277-97.
12. Häuselmann HJ, Aydelotte MB, Schumacher BL, Kuettner KE, Gitelis SH, Thonar EJ. Synthesis and turnover of proteoglycans by human and bovine adult articular chondrocytes cultured in alginate beads. Matrix 1992;12:116-29.
13. Häuselmann HJ, Fernandes RJ, Mok SS, et al. Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads. J Cell Sci 1994;107:17-27.
14. Häuselmann HJ, Masuda K, Hunziker EB, et al. Adult human chondrocytes cultured in alginate form a matrix similar to native human articular cartilage. Am J Physiol 1996-271: C741-52.
15. Humzah MD, Soames RW. Human intervertebral disc: Structure and function. Anat Rec 1988;220:337-56.
16. Johnstone B, Bayliss MT. The large proteoglycans of the human intervertebral disc. Changes in their biosynthesis and structure with age, topography, and pathology. Spine 1995;20: 674-84.
17. Kim YJ, Sah RL, Doong JY, Grodzinsky AJ. Fluorometric assay of DNA in cartilage explants using Hoechst 33258. Anal Biochem 1988;174:168-76.
18. Knudson CB, Knudson W. Hyaluronan-binding protein in development, tissue homeostasis, and disease. FASEB J 1993; 7:1233-41.
19. Kranzler LI, Schaffer L, Sigueira EB. Recent advances in the treatment of ruptured lumbar intervertebral disks. Neurologic Clinics 1985;3:405-16.
20. Kuettner KE, Memoli VA, Pauli BU, Wrobel NC, Thonar EJ, Daniel JC. Synthesis of cartilage matrix by mammalian chondrocytes in vitro. I. Isolation, culture characteristics and morphology. J Cell Biol 1982;93:743-50.
21. Li XQ, Thonar EJ, Knudson W. Accumulation of hyaluronate in human lung carcinoma as measured by a new hyaluronate ELISA. Connect Tissue Res 1989;19:243-53.
22. Maldonado BA, Oegema TJ. Initial characterization of the metabolism of intervertebral disc cells encapsulated in microspheres. J Orthop Res 1992;10:677-90.
23. Masuda K, Shi M, Lenz ME, D'Souza AL, Chiba K, Thonar EJ. Age-related differences in the metabolism of hyaluronan present in two distinct compartments of the matrix formed by articular chondrocytes in vivo. Trans Orthop Res Soc 1995;20: 417.
24. 35S-labeled proteoglycans complexed to alcian blue by rapid filtration in multiwell plates. Anal Biochem 1994;217:167-75.
25. McDevitt CA. Proteoglycans of the intervertebral disc. In: Ghosh P, ed. The Biology of the Intervertebral Disc. Boca Raton, FL: CRC Press, 1988:151-70.
26. Mok SS, Masuda K, Hauselmann HJ, Aydelotte MB, Thonar EJ. Aggrecan synthesized by mature bovine chondrocytes suspended in alginate. Identification of two distinct metabolic matrix pools. J Biol Chem 1994;269:33021-7.
27. Mollenhauer J, Bee JA, Lizarbre MA, von der Mark K. Role of anchorin CII, a 31,000-mol-wt membrane protein, in the interaction of chondrocytes with type II collagen. J Cell Biol 1984;98:1572-8.
28. Momohara S, Masuda K, Lenz ME, et al. Synthesis and turnover of populations of proteoglycans synthesized by adult bovine meniscal cells and articular chondrocytes in alginate beads. Trans Orthop Res Soc 1996;21:17.
29. Nachemson AL. The lumbar spine: An orthopaedic challenge. Spine 1976;1:59-71.
30. Oldberg A, Antonsson P, Lindblom K, Heinegard D. A collagen-binding 59-kd protein (fibromodulin) is structurally related to the small interstitial proteoglycans PG-S1 and PG-S2 (decorin). EMBO J 1989;8:2601-4.
31. Oshima H, Ishihara H, Urban JP, Tsuji H. The use of coccygeal discs to study intervertebral disc metabolism. J Orthop Res 1993;11:332-8.
32. Pearce RH, Grimmer BJ, Adams ME. Degeneration and the chemical composition of the human lumbar intervertebral disc. J Orthop Res 1987;5:198-205.
33. Petit B, Masuda K, D'Souza AL, et al. Characterization of crosslinked collagens synthesized by mature articular chondrocytes cultured in alginate beads: Comparison of two distinct matrix compartments. Exp Cell Res 1996;225:151-61.
34. Scott JE, Bosworth TR, Cribb AM, Taylor JR. The chemical morphology of age-related changes in human intervertebral disc glycosaminoglycans from cervical, thoracic and lumbar nucleus pulposus and annulus fibrosus. J Anat 1994;184: 73-82.
35. Scott JE, Dorlig J. Differential staining of acidic glycosaminoglycans (mucoplysaccharides) by alcian blue in salt solutions. Histochemie 1965;5:221-33.
36. Shinmei M, Kikuchi T, Yamagishi M, Shimomura Y. The role of interleukin-1 on proteoglycan metabolism of rabbit annulus fibrosus cells cultured in vitro. Spine 1988;13:1284-90.
37. Thompson JP, Oegema TJ, Bradford DS. Stimulation of mature canine intervertebral disc by growth factors. Spine 1991;16:253-60.
38. Thonar EJ, Schnitzer TJ, Kuettner KE. Quantification of keratan sulfate in blood as a marker of cartilage catabolism. J Rheumatol 1987;14(suppl):23-4.
39. Thonar EJ, Bjornsson S, Kuetter KE. Age-related changes in cartilage proteoglycans. In: KE Kuettner, Schleyerbach R, Hascall VC, eds. Articular Cartilage Biochemistry. New York: Raven Press, 1986:273-88.
40. Vernon-Roberts B. Disc pathology and disease states. In: Ghosh P, ed. The Biology of the Intervertebral Disc. Boca Raton: CRC Press, 1988:73-120.
41. Yasuma T, Koh S, Okamura T, Yamaguchi Y. Histological changes in aging lumbar intervertebral discs - Their role in protrusion and prolapse. J Bone Joint Surg Am 1990;72: 220-9.