A thermostable phytase from Bacillus sp. MD2: Cloning, expression and high-level production in Escherichia coli

Journal of Industrial Microbiology and Biotechnology

Volumn 37, Issue 3, 2010, Pages 279-287

  Tran, Thi Thuy ^a,b   Mamo, Gashaw ^a   Mattiasson, Bo ^a   Hatti Kaul, Rajni ^a  

^a LUND UNIVERSITY   (Sweden)

^b HANOI NATIONAL UNIVERSITY OF EDUCATION   (Viet Nam)

Author keywords

Alkaline phytase; Bacillus sp.; Fed batch cultivation; Protein secretion

Indexed keywords

CALCIUM CHLORIDE; GLUCOSE; OXYGEN; PHOSPHATE; PHYTASE;

ARTICLE; BACILLUS; BIOREACTOR; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; FED BATCH CULTURE; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN SECRETION; TEMPERATURE SENSITIVITY; THERMOSTABILITY;

6-PHYTASE; BACILLUS; BACTERIAL PROTEINS; BIOREACTORS; CALCIUM CHLORIDE; CLONING, MOLECULAR; CULTURE MEDIA; ENZYME ACTIVATORS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; HOT TEMPERATURE; ISOPROPYL THIOGALACTOSIDE; LACTOSE; PROTEIN STABILITY; RECOMBINANT PROTEINS; TRANSCRIPTIONAL ACTIVATION;

BACILLUS SP.; ESCHERICHIA COLI;

EID: 76849101050     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-009-0671-3     Document Type: Article

References (46)

1. M Åkesson P Hagander P Axelsson 2001 Avoiding acetate accumulation in Escherichia coli cultures using feedback control of glucose feeding Biotechnol Bioeng 73 223 230 10.1002/bit.1054 11257604 (Pubitemid 32381387)
2. M Cheryan 1980 Phytic acid interaction in food systems CRC Crit Rev Food Sci Nutr 13 297 335 10.1080/10408398009527293 1:CAS:528:DyaL3MXivV2juw%3D%3D
3. YM Choi HJ Suh JM Kim 2001 Purification and properties of extracellular phytase from Bacillus sp. KHU-10. J Protein Chem 20 287 292 10.1023/A: 1010945416862 1:CAS:528:DC%2BD3MXnsVemurg%3D (Pubitemid 32927768)
4. S Fu J Sun L Qian Z Li 2008 Bacillus phytases: present scenario and future perspectives Appl Biochem Biotechnol 151 1 8 10.1007/s12010-008-8158-7 1:CAS:528:DC%2BD1cXht1Snt7zF 18785018
5. SP Golovan RG Meidinger A Ajakaiye M Cottrill MZ Weiderkehr D Barney C Plante JW Pollard MZ Fan MA Hayes J Laursen JP Hjorth RR Hacker JP Phillips CW Forsberg 2001 Pigs expressing salivary phytase produce low-phosphorus manure Nature Biotech 19 741 745 10.1038/90788 1:CAS:528:DC%2BD3MXlslekt78%3D (Pubitemid 32729535)
6. AK Gombert BV Kilikian 1998 Recombinant gene expression in Escherichia coli cultivation using lactose as inducer J Biotechnol 60 47 54 10.1016/S0168-1656(97)00185-5 1:CAS:528:DyaK1cXhvVSit7k%3D 9571801 (Pubitemid 28122303)
7. HK Gulati BS Chadha HS Saini 2007 Production and characterization of thermostable alkaline phytase from Bacillus laevolacticus isolated from rhizosphere soil J Indust Microbiol Biotechnol 34 91 98 1:CAS:528: DC%2BD28XhtlShs7fP
8. A Hara S Ebina A Kondo T Funagua 1985 A new type of phytase from Typha latifolia L Agric Biol Chem 49 3539 3544 1:CAS:528:DyaL28XkslKjsA%3D%3D
9. T Holm S Arvidson B Lindholm B Pavlu 1970 Enzyme laboratory-scale production Process Biochem 5 62 66
10. U Horn W Strittmatter A Krebber U Knupfer M Kujau R Wenderoth K Muller S Matzku A Pluckthun D Riesenberg 1996 High volumetric yield of functional dimeric miniantibodies in Escherichia coli, using an optimized expression vector and high-cell-density fermentation under non-limited growth condition Appl Microbiol Biotechnol 46 524 532 10.1007/s002530050855 1:CAS:528:DyaK2sXht1Sqtb8%3D 9008885 (Pubitemid 26426411)
11. RE Huber G Kurz K Wallenfels 1976 A quantitation of the factors which affect the hydrolase and transgalactosidase activities of -galactosidase (E. coli) on lactose Biochem 15 1994 2001 10.1021/bi00654a029 1:CAS:528: DyaE28XktFKhs70%3D
12. J Kerovuo M Lauraeus P Nurminen N Kalkkinen J Apajalahti 1998 Isolation, characterization, molecular gene cloning and sequencing of novel phytase from Bacillus subtilis Appl Environ Microbiol 64 2079 2085 1:CAS:528: DyaK1cXjslWhsLo%3D 9603817
13. J Kerovuo N Von Weymarn M Povelainen S Auer A Miasnikov 2000 A new efficient expression system for Bacillus and its application to production of recombinant phytase Biotech Lett 22 1311 1317 10.1023/A:1005694731039 1:CAS:528:DC%2BD3cXnt1aqt70%3D
14. YO Kim HK Kim KS Bae JH Yu TK Oh 1998 Purification and properties of a thermostable phytase from Bacillus sp. DS11 Enzyme Microb Technol 22 2 7 10.1016/S0141-0229(97)00096-3 1:CAS:528:DyaK2sXotVSiur4%3D (Pubitemid 28033398)
15. YO Kim HK Kim JK Lee JH Yu TK Oh 1998 Cloning of the thermostable phytase gene (phy) from Bacillus DS11 and its over expression in Escherichia coli FEMS Microbiol Lett 162 185 191 10.1111/j.1574-6968.1998.tb12997.x 1:CAS:528:DyaK1cXisFalt7o%3D 9595681
16. DH Kim BC Oh WC Choi JK Lee TK Oh 1999 Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive Biotech Lett 21 925 927 10.1023/A:1005602717835 1:CAS:528:DyaK1MXotFSit7c%3D (Pubitemid 30040986)
17. YO Kim JK Lee BC Oh TK Oh 1999 High-level expression of a recombinant thermostable phytase in Bacillus subtilis Biosci Biotechnol Biochem 63 2205 2207 10.1271/bbb.63.2205 1:CAS:528:DC%2BD3cXlsFKktQ%3D%3D
18. S Kleist G Miksch B Hitzmann M Arndt K Friehs E Flaschel 2003 Optimization of the extracellular production of a bacterial phytase with Escherichia coli by using different fed-batch fermentation strategies Appl Microbiol Biotechnol 61 456 462 1:CAS:528:DC%2BD3sXjvFClt7w%3D 12764560
19. SF Lassen J Breinholt PR Ostergaard R Brugger A Bischoff M Wyss CC Fuglsang 2001 Expression, gene cloning, and characterization of five novel phytase from four Basidomycete fungi: Peniophora lycii, Agrocybe pediades a Ceriporia sp., and Trametes pubescens Appl Environ Microbiol 67 4701 4707 10.1128/AEM.67.10.4701-4707.2001 1:CAS:528:DC%2BD3MXns1Witrs%3D 11571175
20. HW Lopez F Leenhardt C Coudray C Remesy 2002 Mineral and phytic acid interactions: is it a real problem for human nutrition? Int J Food Sci Tech 37 727 739 10.1046/j.1365-2621.2002.00618.x 1:CAS:528:DC%2BD38XotFygtL0%3D (Pubitemid 36840478)
21. VT Maddaiah AA Kurnick BL Reid 1964 Phytic acid studies Proc Soc Exp Biol Med 115 391 393 1:CAS:528:DyaF2cXnslentQ%3D%3D 14121619
22. DD Maenz CM Engele-Schaan RW Newkirk HL Classen 1999 The effect of minerals and mineral chelators on the formation of phytase-resistant and phytase-susceptible forms of phytic acid in solution and in a slurry of canola meal Anim Feed Sci Technol 81 177 192 10.1016/S0377-8401(99)00085-1 1:CAS:528:DyaK1MXmsVWmur4%3D (Pubitemid 29485487)
23. Mallin MA (2000) Impact of industrial animal production on river and estuaries. Am Sci (January-February) 88:26-73
24. G Miksch S Kleist K Frieh E Flaschel 2002 Overexpression of phytase from Escherichia coli and its extracellular production in bioreactors Appl Microbiol Biotechnol 59 685 694 10.1007/s00253-002-1071-z 1:CAS:528:DC%2BD38XoslyktLk%3D 12226725
25. B Muller-Hill HV Rickenberg K Wallenfels 1964 Specificity of the induction of the enzymes of the lac operon in Escherichia coli J Mol Biol 10 303 318 10.1016/S0022-2836(64)80049-8
26. Y Ni R Chen 2009 Extracellular recombinant protein production from Escherichia coli Biotechnol Lett 31 1661 1670 10.1007/s10529-009-0077-3 1:CAS:528:DC%2BD1MXht1eksb7O 19597765
27. 2+) solubilities in the presence of phytate J Sci Food Agric 40 79 85 10.1002/jsfa.2740400110 1:CAS:528:DyaL2sXltleisbc%3D
28. BC Oh WC Choi S Park YO Kim TK Oh 2004 Biochemical properties and substrate specificities of alkaline and histidine acid phytase Appl Microbiol Biotechnol 63 362 372 10.1007/s00253-003-1345-0 1:CAS:528:DC%2BD2cXitF2isQ%3D%3D 14586576 (Pubitemid 38134773)
29. JM Pages J Anba C Lazdunski 1987 Conditions leading to secretion of a normally periplasmic protein in Escherichia coli J Bacteriol 169 1386 1390 1:CAS:528:DyaL2sXhslalurk%3D 3549684
30. V Purva CB Uttam 2004 Production studies and catalytic properties of phytases (myo-inositolhexakisphosphate phosphohydrolase): an overview Enzyme Micob Technol 35 3 4 10.1016/j.enzmictec.2004.03.010 (Pubitemid 38759788)
31. SO Ramchuran E Nordberg Karlsson S Velut L De Mare P Hagander O Holst 2002 Production of heterologous thermostable glycoside hydrolases and presence of host-cell proteases in substrate limited fed-batch cultures of Escherichia coli BL21(DE3) Appl Microbiol Biotechnol 60 408 416 10.1007/s00253-002-1132-3 1:CAS:528:DC%2BD38Xpt1Cjurs%3D 12466880
32. SO Ramchuran E Nordberg Karlsson O Holst 2003 Effect of post induction, nutrient feed composition and use of lactose as inducer during production of thermostable xylanase in Escherichia coli J Biosci Bioeng 99 477 484 10.1263/jbb.99.477
33. DECS Rao KV Rao VD Reddy 2008 Cloning and expression of Bacillus phytase gene (phy) in Escherichia coli and recovery of active enzyme from the inclusion bodies J Appl Microbiol 105 1128 1137 10.1111/j.1365-2672.2008.03833.x 1:CAS:528:DC%2BD1cXht12lurnE 18479345
34. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
35. JJ Scott FA Loewus 1986 A calcium-activated phytase from pollen of Lilium longiflorum Plant Physiol 82 333 335 10.1104/pp.82.1.333 1:CAS:528: DyaL28XlvVKntrY%3D 16665018
36. P Shah K Bhavsar SK Soni JM Khire 2009 Strain improvement and up scaling of phytase production by Aspergillus niger NCIM 563 under submerged fermentation conditions J Ind Microbiol Biotechnol 36 373 380 10.1007/s10295-008-0506-7 1:CAS:528:DC%2BD1MXitlWrsL0%3D 19082644
37. M Shimizu 1992 Purification and characterization of phytase from Bacillus subtillis (nato) N-77 Biosci Biotech Biochem 56 1266 1269 10.1271/bbb.56.1266 1:CAS:528:DyaK38Xls1Kns74%3D
38. O Simon F Igbasan 2002 In vitro properties of phytase from various microbial origins Inter J Food Sci Tech 37 813 822 10.1046/j.1365-2621.2002. 00621.x 1:CAS:528:DC%2BD38XotFygtb4%3D (Pubitemid 36840485)
39. CH Stahl DB Wilson XG Lei 2003 Comparison of extracellular Escherichia coli AppA phytase expressed in Streptomyces lividans and Pichia pastoris Biotech Lett 25 827 831 10.1023/A:1023568826461 1:CAS:528:DC%2BD3sXjt1Ogu78%3D (Pubitemid 36614890)
40. K Sunitha JK Lee TK Oh 1999 Optimization of medium components for phytase production by E. coli using response surface methodology Bioproc Eng 21 477 481 1:CAS:528:DyaK1MXnsVajs7c%3D
41. I Suominen M Karp M Lahde A Kopio T Glumoff P Meyer P Mantsala 1987 Extracellular production of cloned α-amylase by Escherichia coli Gene 61 165 176 10.1016/0378-1119(87)90111-9 1:CAS:528:DyaL1cXht1Grsb0%3D 3327755 (Pubitemid 18067592)
42. MI Viitanen A Vasala P Neubauer T Alatossava 2003 Cheese whey-induced high-cell-density production of recombinant proteins in Escherichia coli Microb Cell Fact 2 2 10.1186/1475-2859-2-2 12740045 (Pubitemid 38751439)
43. A Vohra T Satyanarayana 2004 A cost-effective molasses medium for enhanced cell-bound phytase production by Pichia anomala J Appl Microbiol 97 471 476 10.1111/j.1365-2672.2004.02327.x 1:CAS:528:DC%2BD2cXoslGktLg%3D 15281926 (Pubitemid 39208187)
44. A Vuolanto N Von Weymarn J Kerovuo H Ojamo M Leisola 2001 Phytase production by high cell density culture of recombinant Bacillus subtilis Biotech Lett 23 761 766 10.1023/A:1010369325558 1:CAS:528:DC%2BD3MXltVGrtLs%3D (Pubitemid 32578552)
45. M Wyss R Brugger A Kronenberger R Remy R Fimbel G Oesterhelt M Lehmann AP Van Loon 1999 Biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties Appl Environ Microbiol 65 367 373 1:CAS:528:DyaK1MXpvFCntw%3D%3D 9925555
46. M Yamabhai S Emrat S Sukasem P Pesatcha N Jaruseranee B Buranabanyat 2008 Secretion of recombinant Bacillus hydrolytic enzymes using Escherichia coli expression systems J Biotechnol 133 50 57 10.1016/j.jbiotec.2007.09.005 1:CAS:528:DC%2BD2sXhtlals7%2FM 17950946 (Pubitemid 350101872)