Cloning of the thermostable phytase gene (phy) from Bacillus sp. DS11 and its overexpression in Escherichia coli

FEMS Microbiology Letters

Volumn 162, Issue 1, 1998, Pages 185-191

  Kim, Young Ok ^a,b   Lee, Jung Kee ^a   Kim, Hyung Kwoun ^a   Yu, Ju Hyun ^b   Oh, Tae Kwang ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Yonsei University   (South Korea)

Author keywords

Bacillus; Overexpression; Phytase; Phytate

Indexed keywords

AMINO ACID; CYANOGEN BROMIDE; DNA; NUCLEOTIDE; OLIGONUCLEOTIDE; PHOSPHATE; PHOSPHORUS; PHYTASE; PHYTATE; POLYPEPTIDE; PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS; ESCHERICHIA COLI; GENE; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SOUTHERN BLOTTING;

6-PHYTASE; AMINO ACID SEQUENCE; BACILLUS; BASE SEQUENCE; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; HEAT; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; RECOMBINANT FUSION PROTEINS; RESTRICTION MAPPING; SEQUENCE ANALYSIS; SEQUENCE ANALYSIS, DNA;

EID: 0032080595     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(98)00119-0     Document Type: Article

References (23)

1. Lolas, G.M. and Markakis, P. (1997) Phytase of navy beans. J. Food Sci. 42, 1094-1097.
2. Sharma, C.B., Goel, M. and Irshad, M. (1978) Myo-inositol hexaphosphate as a potential inhibitor of α-amylases of different origins. Phytochemistry 17, 201-204.
3. Graf, E. (Ed.) (1986) Phytic Acid, Chemistry and Applications. Pillsbury, Pilatus Press, Minneapolis, MN.
4. Yamada, K., Minoda, Y. and Yamamoto, S. (1986) Phytase from Aspergillus terreus. Part I. Production, purification and some general properties of the enzyme. Agric. Biol. Chem. 32, 1275-1282.
5. Gibson, D. (1987) Production of extracellular phytase from Aspergillus ficuum on starch media. Biotechnol. Lett. 9, 305-310.
6. Shieh, T.R. and Ware, J.H. (1968) Survey of microorganisms for the production of extracellular phytase. Appl. Microbiol. 6, 1348-1351.
7. Greaves, M.P., Anderson, G. and Webley, D.M. (1967) Hydrolysis of inositol phosphates by Aerobacter aerogenes. Biochim. Biophys. Acta 132, 412-418.
8. Cosgrove, D.J., Irving, G.C.J. and Bromfield, S.M. (1970) Inositol phosphate phosphatases of microbiological origin. The isolation of soil bacteria having inositol phosphate phosphatase activity. Aust. J. Biol. Sci. 23, 339-343.
9. Greiner, R., Konietzny, U. and Jany, K.I.-D. (1993) Purification and characterization of two phytase from Escherichia coli. Arch. Biochem. Biophys. 303, 107-113.
10. Powar, V.K. and Jagannathan, V. (1982) Purification and properties of phytate-specific phosphatase from Bacillus subtilis. J. Bacteriol. 151, 1102-1108.
11. Shimizu, M. (1992) Purification and characterization of phytase from Bacillus subtilis (natto) N-77. Biosci. Biotechnol. Biochem. 56, 1266-1269.
12. Van Hartingseldt, W., Van Zeijl, C.M.J., Harteveld, G.M., Gouka, R.J. Suykerbuyk, M.E.G., Luiten, R.G.M., Van Paridon, P.A., Selten, G.C.M., Veenstra, A.E., Van Gorcom, R.F.M. and Van den Hondel, C.A.M.J.J. (1993) Cloning, characterization and overexpression of the phytase-encoding gene (phy A) of Aspergillus niger. Gene 127, 87-94.
13. Ehrlich, K.C., Montalbano, B.G., Mullaney, E.J., Dishinger, H.C. and Ullah, A.H.J. (1993) Identification and cloning of a second phytase gene (phy B) from Aspergillus niger (ficuum), Biochem. Biophys. Res. Commun. 195, 53-57.
14. Piddington, C.S., Paloheimo, H.M., Cantrell, M., Miettinen-Oinonen, A., Nevalainen, H. and Rambosek, J. (1993) The cloning and sequencing of the genes encoding phytase (phy) and pH 2.5-optimum acid phosphatase (aph) from Aspergillus niger ver. awamori. Gene 133, 55-62.
15. Pasamontes, L., Haiker, M., Wyss, M., Tessier, M. and Van Loon, A.P.G.M. (1997) Gene cloning, purification, and characterization of a heat stable phytase from the fungus Aspergillus fumigatus. Appl. Environ. Microbiol. 63, 1696-1700.
16. Mitchell, D.B., Vogel, K., Weimann, B.J., Pasamontes, L. and Van Loon, A.P.G.M. (1997) The phytase subfamily of histidine acid phosphatase: isolation of genes for two novel phytases from the fungi Aspergillus terreus and Myceliophthora thermophila. Microbiology 143, 245-252.
17. Kim, Y.O., Kim, H.K., Yu, J.H. and Oh, T.K. (1997) Purification and properties of a thermostable phytase from Bacillus sp. DS11 enzyme. Microb. Technol. 22, 2-7.
18. Marmur, J. (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3, 208-218.
19. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
20. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5468.
21. Das, A., Merril, C. and Adhya, S. (1978) Interaction of RNA polymerase and rho in transcription termination: coupled ATPase. Proc. Natl. Acad. Sci. USA 75, 4828-4832.
22. Roels, S. and Losick, R. (1995) Adjacent and divergently oriented operons under the control of the sporulation regulatory protein GerE in Bacillus subtilis. J. Bacteriol. 177, 6763-6275.
23. Neubauer, P., Hofmann, K., Holst, O., Mattiasson, B. and Kruschke, P. (1992) Maximizing the expression of a recombinant gene in Escherichia coli by manipulation of induction time using lactose as inducer. Appl. Microbiol. Biotechnol. 36, 739-744.