Cloning, overexpression, folding and purification of a biosynthetically derived three disulfide scorpion toxin (BTK-2) from Mesobuthus tamulus

Protein Expression and Purification

Volumn 70, Issue 2, 2010, Pages 137-142

  Kumar, G Senthil ^a   Sarma, Siddhartha P ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

Animal toxins; Biosynthetic production; Disulfide rich peptides; Fusion proteins; Heteronuclear NMR; Isotopic labeling

Indexed keywords

BTK 2 TOXIN, MESOBUTHUS TAMULUS; BTK-2 TOXIN, MESOBUTHUS TAMULUS; DISULFIDE; PEPTIDE; PROTEINASE; SCORPION VENOM; TEV PROTEASE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; GEL CHROMATOGRAPHY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE; PROTEIN FOLDING; SCORPION;

AMINO ACID SEQUENCE; ANIMALS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; DISULFIDES; ENDOPEPTIDASES; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PEPTIDES; PROTEIN FOLDING; SCORPION VENOMS; SCORPIONS;

ANIMALIA; ESCHERICHIA COLI; MESOBUTHUS TAMULUS; SCORPIONES;

EID: 76749161359     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.10.017     Document Type: Article

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