Development of a bacterial cloning vector for expression of scorpion toxins for biotechnological studies

Protein Expression and Purification

Volumn 57, Issue 1, 2008, Pages 88-94

  Stehling, Eliana Guedes ^a   da Silveira, Wanderley Dias ^a   Campos, Tatiana Amabile ^a   Brocchi, Marcelo ^a   Pertinhez, Thelma Aguiar ^b   Spisni, Alberto ^b  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b UNIVERSITY OF PARMA   (Italy)

Author keywords

Molecular cloning; Scorpion; Toxin

Indexed keywords

EPITOPE; HISTIDINE; OLIGONUCLEOTIDE; PROTEINASE; SCORPION VENOM; VIRUS FUSION PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BACTERIOPHAGE LAMBDA; BACTERIUM; BACTERIUM TRANSFORMATION; BIOSYNTHESIS; BIOTECHNOLOGY; CHEMISTRY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PLASMID; SCORPION; SOLUBILITY; VIRUS CAPSID;

AMINO ACID SEQUENCE; ANIMALS; BACTERIA; BACTERIOPHAGE LAMBDA; BASE SEQUENCE; BIOTECHNOLOGY; CAPSID; CLONING, MOLECULAR; ENDOPEPTIDASES; EPITOPES; ESCHERICHIA COLI; GENETIC VECTORS; HISTIDINE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OLIGONUCLEOTIDES; PLASMIDS; SCORPION VENOMS; SCORPIONS; SOLUBILITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRANSFORMATION, BACTERIAL; VIRAL FUSION PROTEINS;

BACTERIA (MICROORGANISMS); ENTEROBACTERIA PHAGE LAMBDA; MIRIDAE; SCORPIONES; TOBACCO ETCH VIRUS;

EID: 36248995795     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.08.001     Document Type: Article

References (22)

1. Catterall W.A. Neurotoxins that act on voltage-sensitive sodium channels in excitable membranes. Ann. Rev. Pharmacol. Toxicol. 20 (1980) 15-43
2. Catterall W.A., and Epstein P.N. Ion chan. Diabetol. 35 (1992) 23-33
3. Gordon D., Saravin P., Gurevitz M., and Zinn-Justin S. Functional anatomy of scorpion toxins affecting sodium channels. J. Toxicol. Toxin Rev. 17 (1998) 131-159
4. Gordon D., Martin-Eauclaire M.F., Cestele S., Kopeyan C., Carlier E., Ben Kalifa R., Pelhate M., and Rochat H. Scorpion toxins affecting sodium current inactivation bind to distinct homologous receptor sites on rat brain and insect sodium channels. J. Biol. Chem. 271 (1996) 8034-8045
5. Miller C. Charybdotoxin family of K+ channel-blocking peptides. Neuron 15 (1995) 5-10
6. Possani L.D., Becerril B., Delepierre M., and Tytgat J. Scorpion toxins specific for Na+ channels. Eur. J. Biochem. 264 (1999) 287-300
7. Garcia M.L., Hanner M., Knaus H.G., Koch R., Schmalhofer W., Slaughter R.S., and Kaczorowski G.J. Pharmacology of potassium channels. Adv. Pharmacol. 39 (1997) 425-471
8. Tytgat J., Chandy K.G., Garcia M.L., Gutman G.A., Martin-Eauclaire M.F., van der Walt J.J., and Possani L.D. An unified nomenclature for short-chain peptides isolated from scorpion venoms: alpha-KTx molecular subfamilies. Trends Pharmacol. Sci. 11 (1999) 444-447
9. Legros C., Ceard B., Bougis P.E., and Martin-Eauclaire M.F. Evidence for a new class of scorpion toxins active against K1 channels. FEBS Lett. 431 (1998) 375-380
10. Gurrola G.B., Rosati B., Rocchetti M., Pimienta G., Zaza A., Arcangeli A., Olivotto M., Possani L.D., and Wanke E. A toxin to nervous, cardiac, and endocrine ERG K1 channels isolated from Centruroides noxius scorpion venom. FASEB J. 13 (1999) 953-962
11. Abdel-Mottaleb Y., Coronas F., de Roodt A., Possani L., and Tytgat J. A novel toxin from the venom of the scorpion Tityus trivittatus is the first member of a new α-KTX subfamily. FEBS Lett. 580 (2006) 592-596
12. Giangiacomo K.M., Garcia M.L., and McManus O.B. Mechanism of iberiotoxin block of the large-conductance calcium-activated potassium channel from bovine aortic smooth muscle. Biochemistry 31 (1992) 6719-6727
13. Forrer P., and Jaussi R. High-level expression of soluble heterologous proteins in the cytoplasm of Escherichia coli by the fusion to the bacteriophage lambda head protein D. Gene 224 (1998) 45-52
14. Prodromou C., and Pearl L.H. Recursive PCR: a novel technique for total gene synthesis. Protein Eng. 5 (1992) 827-829
15. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: a Laboratory Manual (1989), Cold Spring Harbour, NewYork
16. Kelly S.M., and Price N.C. The use of circular dichroism in the investigation of protein structure and function. Curr. Protein Pept. Sci. 1 (2000) 349-384
17. Phan J., Zdanov A., Evdokimov A.G., Tropea J.E., Peters H.P.K., Kapust R.B., Li M., Wlodawer A., and Waugh D.S. Structural basis for the substrate specificity of tobacco etch virus protease. J. Biol. Chem. 277 (2002) 50564-50572
18. Fu Y.J., Yin L.Y., Wang W., Chai B.F., and Liang A.H. Synthesis, expression and purification of a type of chlorotoxin-like peptide from the scorpion, Buthus martensii Karsch, and its acute toxicity analysis. Biotechnol. Lett. 27 (2005) 1597-1603
19. Yu J.B., Ji P., Zha X.M., Shen W.D., and Wu X.F. Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli. Sheng. Wu. Gong. Cheng. Xue. Bao. 18 (2002) 106-108
20. Selisko B., Cosío G., García C., Becerril B., Possani L.D., and Horjales E. Bacterial expression, purification and functional characterization of a recombinant chimeric Fab derived from murine mAb BCF2 that neutralizes the venom of the scorpion Centruroides noxius Hoffmann. Toxicon 43 (2004) 43-51
21. Devaux C., Moreau E., Goyffon M., Rochat H., and Billiald P. Construction and functional evaluation of a single-chain antibody fragment that neutralizes toxin AahI from the venom of the scorpion Androctonus australis hector. Eur. J. Biochem. 268 (2001) 694-702
22. Rider H.C., Kupryszewski G., Rekowski P., and Lammek B. Origin of the positive 225-230 nm circular dichroism band in proteins. Its application to conformational analysis. Biophys. Chem. 31 (1988) 45-51