Conditional c-myb knockout in adult hematopoietic stem cells leads to loss of self-renewal due to impaired proliferation and accelerated differentiation

Proceedings of the National Academy of Sciences of the United States of America

Volumn 106, Issue 51, 2009, Pages 21689-21694

  Lieu, Yen K ^a   Reddy, E Premkumar ^a  

^a TEMPLE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Bone marrow; Development; Hematopoiesis; Proto oncogene

Indexed keywords

METHYLCELLULOSE; TRANSCRIPTION FACTOR;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BLOOD CELL; BONE MARROW; CELL DIFFERENTIATION; CELL PROLIFERATION; CONTROLLED STUDY; GENE EXPRESSION; HEMATOPOIESIS; HEMATOPOIETIC STEM CELL; IN VITRO STUDY; MEGAKARYOCYTE; MOUSE; NONHUMAN; ONCOGENE C MYB; PRIORITY JOURNAL; PROTO ONCOGENE;

ANIMALS; CELL DIFFERENTIATION; CELL PROLIFERATION; GENE EXPRESSION; HEMATOPOIETIC STEM CELLS; MICE; MICE, KNOCKOUT; PROTO-ONCOGENE PROTEINS C-MYB;

MUS;

EID: 76049120037     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0907623106     Document Type: Article

References (28)

1. Kondo M, et al. (2003) Biology of hematopoietic stem cells and progenitors: implications for clinical application. Annu Rev Immunol 21:759-806.
2. Baluda MA, Reddy EP (1994) Anatomy of an integrated avian myeloblastosis provirus: structure and function. Oncogene 9:2761-2774.
3. Mucenski ML, et al. (1991) A functional c-myb gene is required for normal murine fetal hepatic hematopoiesis. Cell 65:677-689.
4. Oh IH, Reddy EP (1999) The myb gene family in cell growth, differentiation and apoptosis. Oncogene 18:3017-3033.
5. Thomas MD, Kremer CS, Ravichandran KS, Rajewsky K, Bender TP (2005) c-Myb is critical for B cell development and maintenance of follicular B cells. Immunity 23:275-286.
6. Bender TP, Kremer CS, Kraus M, Buch T, Rajewsky K (2004) Critical functions for c-Myb at three checkpoints during thymocyte development. Nat Immunol 5:721-729.
7. Badiani P, Corbella P, Kioussis D, Marvel J, Weston K (1994) Dominant interfering alleles define a role for c-Myb in T-cell development. Genes Dev 8:770-782.
8. Pearson R, Weston K (2000) c-Myb regulates the proliferation of immature thymocytes following beta-selection. EMBO J 19:6112-6120.
9. Lieu YK, Kumar A, Pajerowski AG, Rogers TJ, Reddy EP (2004) Requirement of c-myb in T cell development and in mature T cell function. Proc Natl Acad Sci USA 101:14853-14858.
10. Xiao C, et al. (2007) MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell 131:146-159.
11. Garcia P, et al. (2009) Reduced c-Myb activity compromises HSCs and leads to a myeloproliferation with a novel stem cell basis. EMBO J 28:1492-1504.
12. Sandberg ML, et al. (2005) c-Myb and p300 regulate hematopoietic stem cell proliferation and differentiation. Dev Cell 8:153-166.
13. Kuhn R, Schwenk F, Aguet M, Rajewsky K (1995) Inducible gene targeting in mice. Science 269:1427-1429.
14. Kiel MJ, Yilmaz OH, Iwashita T, Terhorst C, Morrison SJ (2005) SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell 121:1109-1121.
15. Sicurella C, et al. (2001) Defective stem cell factor expression in c-myb null fetal liver stroma. Blood Cells Mol Dis 27:470-478.
16. Mikkola HK, et al. (2003) Haematopoietic stem cells retain long-term repopulating activity and multipotency in the absence of stem-cell leukaemia SCL/tal-1 gene. Nature 421:547-551.
17. Ness SA (1999) Myb binding proteins: regulators and cohorts in transformation. Oncogene 18:3039-3046.
18. Emambokus N, et al. (2003) Progression through key stages of haemopoiesis is dependent on distinct threshold levels of c-Myb. EMBO J 22:4478-4488.
19. Morrison SJ, Hemmati HD, Wandycz AM, Weissman IL (1995) The purification and characterization of fetal liver hematopoietic stem cells. Proc Natl Acad Sci USA 92:10302-10306.
20. Allen RD III, Bender TP, Siu G (1999) c-Myb is essential for early T cell development. Genes Dev 13:1073-1078.
21. Sumner R, Crawford A, Mucenski M, Frampton J (2000) Initiation of adult myelopoiesis can occur in the absence of c-Myb whereas subsequent development is strictly dependent on the transcription factor. Oncogene 19:3335-3342.
22. Hock H, et al. (2004) Gfi-1 restricts proliferation and preserves functional integrity of haematopoietic stem cells. Nature 431:1002-1007.
23. Peled A, et al. (1999) Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4. Science 283:845-848.
24. Wilson A, et al. (2004) c-Myc controls the balance between hematopoietic stem cell self-renewal and differentiation. Genes Dev 18:2747-2763.
25. Domen J, Cheshier SH, Weissman IL (2000) The role of apoptosis in the regulation of hematopoietic stem cells: Overexpression of Bcl-2 increases both their number and repopulation potential. J Exp Med 191:253-264.
26. Miller CL, et al. (1996) Studies of W mutant mice provide evidence for alternate mechanisms capable of activating hematopoietic stem cells. Exp Hematol 24:185-194.
27. Sharma Y, Astle CM, Harrison DE (2007) Heterozygous kit mutants with little or no apparent anemia exhibit large defects in overall hematopoietic stem cell function. Exp Hematol 35:214-220.
28. Pear WS, et al. (1998) Efficient and rapid induction of a chronic myelogenous leukemialike myeloproliferative disease in mice receiving P210 bcr/abl-transduced bone marrow. Blood 92:3780-3792.