Molecular cytogenetic characterization of the human cerebral microvessel endothelial cell line hCMEC/D3

Cytogenetic and Genome Research

Volumn 126, Issue 4, 2010, Pages 313-317

  Mkrtchyan, H ^a   Scheler, S ^b,c   Klein, I ^b   Fahr, A ^b   Couraud, P O ^d   Romero, I A ^d   Weksler, B ^d   Liehr, T ^a  

^a Jena University Hospital   (Germany)

^b FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^c NOVARTIS PHARMA AG   (Switzerland)

^d INSERM   (France)

Author keywords

HCMEC D3; Karyotype; Microvessel endothelial cell line; Multicolor banding (MCB)

Indexed keywords

ARTICLE; BLOOD BRAIN BARRIER; CELL LINE; CEREBRAL MICROVESSEL ENDOTHELIAL CELL LINE; CHROMOSOME ABERRATION; CHROMOSOME REARRANGEMENT; CYTOGENETICS; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; KARYOTYPE; PRIORITY JOURNAL; BRAIN; CYTOLOGY; ENDOTHELIUM CELL; KARYOTYPING; MICROVASCULATURE; VASCULARIZATION;

BRAIN; CELL LINE; CHROMOSOME ABERRATIONS; ENDOTHELIAL CELLS; HUMANS; KARYOTYPING; MICROVESSELS;

EID: 75749114784     PISSN: 14248581     EISSN: None     Source Type: Journal    
DOI: 10.1159/000253080     Document Type: Article

References (27)

1. Camps J, Mrasek K, Prat E, Weise A, Starke H, et al: Molecular cytogenetic characterisation of the colorectal cancer cell line SW480. Oncol Rep 11: 1215-1218 (2004).
2. Chattopadhyay N, Zastre J, Wong HL, Wu XY, Bendayan R: Solid lipid nanoparticles enhance the delivery of the HIV protease inhibitor, atazanavir, by human brain endothelial cell line. Pharm Res 25: 2262-2271 (2008).
3. Cucullo L, Couraud PO, Weksler B, Romero IA, Hossain M, et al: Immortalized human brain endothelial cells and flow-based vascular modeling: a marriage of convenience for rational neurovascular studies. J Cereb Blood Flow Metab 28: 312-328 (2008).
4. Dauchy S, Miller F, Couraud PO, Weaver RJ, Weksler B, et al: Expression and transcriptional regulation of ABC transporters and cytochromes P450 in hCMEC/D3 human cerebral microvascular endothelial cells. Biochem Pharmacol 77: 897-909 (2009).
5. Fischer S, Nishio M, Peters SC, Tschernatsch M, Walberer M, et al: Signalling mechanism of extracellular RNA in endothelial cells. FASEB J 23: 2100-2109 (2009).
6. Huang W, Eum SY, András IE, Hennig B, Toborek M: PPARalpha and PPARgamma attenuate HIV-induced dysregulation of tight junction proteins by modulations of matrix metalloproteinase and proteasome activities. FASEB J 23: 1596-1606 (2009).
7. Huang W, Rha GB, Han MJ, Eum SY, András IE, et al: PPARalpha and PPARgamma effectively protect against HIV-induced inflammatory responses in brain endothelial cells. J Neurochem 107: 497-509 (2008).
8. Hurst LA, Bunning RA, Couraud PO, Romero IA, Weksler BB, et al: Expression of ADAM-17, TIMP-3 and fractalkine in the human adult brain endothelial cell line, hCMEC/ D3, following pro-inflammatory cytokine treatment. J Neuroimmunol 210: 108-112 (2009).
9. ISCN 2009: An International System for Human Cytogenetic Nomenclature. Shaffer LG, Slovak ML, Campbell LJ (eds). (S Karger, Basel 2009).
10. Karst C, Trifonov V, Romanenko SA, Claussen U, Mrasek K, et al: Molecular cytogenetic characterization of the mouse cell line WMP2 by spectral karyotyping and multicolor banding applying murine probes. Int J Mol Med 17: 209-213 (2006).
11. Kautenburger T, Beyer-Sehlmeyer G, Festag G, Haag N, Kühler S, et al: The gut fermentation product butyrate, a chemopreventive agent, suppresses glutathione S-transferase theta (hGSTT1) and cell growth more in human colon adenoma (LT97) than tumor (HT29) cells. J Cancer Res Clin Oncol 131: 692-700 (2005).
12. Kuechler A, Weise A, Michel S, Schaeferhenrich A, Pool-Zobel BL, et al: Precise breakpoint characterization of the colon adenocarcinoma cell line HT-29 clone 19A by means of 24-color fluorescence in situ hybridization and multicolor banding. Genes Chromosomes Cancer 36: 207-210 (2003).
13. Liehr T, Claussen U: Multicolor-FISH approaches for the characterization of human chromosomes in clinical genetics and tumor cytogenetics. Curr Genomics 3: 213-235 (2002).
14. Lim JC, Kania KD, Wijesuriya H, Chawla S, Sethi JK, et al: Activation of beta-catenin signaling by GSK-3 inhibition increases P-glycoprotein expression in brain endothelial cells. J Neurochem 106: 1855-1865 (2008).
15. Mrasek K, Heller A, Rubtsov N, Trifonov V, Starke H, et al: Reconstruction of the female Gorilla gorilla karyotype using 25-color FISH and multicolor banding (MCB). Cytogenet Cell Genet 93: 242-248 (2001).
16. Poller B, Gutmann H, Krähenbühl S, Weksler B, Romero I, et al: The human brain endothelial cell line hCMEC/D3 as a human bloodbrain barrier model for drug transport studies. J Neurochem 107: 1358-1368 (2008).
17. Rosenhahn J, Weise A, Michel S, Hennig K, Hartmann I, et al: Cytogenetic characterisation and proteomic profiling of the Imatinib-resistant cell line KCL22-R. Int J Oncol 31: 121-128 (2007).
18. Sade H, Holloway K, Romero IA, Male D: Transcriptional control of occluding expression in vascular endothelia: regulation by Sp3 and YY1. Biochim Biophys Acta 1789: 175-184 (2009).
19. Schaeferhenrich A, Beyer-Sehlmeyer G, Festag G, Kuechler A, Haag N, et al: Human adenoma cells are highly susceptible to the genotoxic action of 4-hydroxy-2-nonenal. Mutat Res 526: 19-32 (2003a).
20. Schaeferhenrich A, Sendt W, Scheele J, Kuechler A, Liehr T, et al: Putative colon cancer risk factors damage global DNA and TP53 in primary human colon cells isolated from surgical samples. Food Chem Toxicol 41: 655-664 (2003b).
21. Tai LM, Holloway KA, Male DK, Loughlin AJ, Romero IA: Amyloid-beta-induced occluding down-regulation and increased permeability in human brain endothelial cells is mediated by MAPK activation. J Cell Mol Med 2. Apr. Epub ahead of print (2009a)
22. Tai LM, Loughlin AJ, Male DK, Romero IA: Pglycoprotein and breast cancer resistance protein restrict apical-to-basolateral permeability of human brain endothelium to amyloid-beta. J Cereb Blood Flow Metab 29: 1079-1083 (2009b).
23. Weise A, Heller A, Starke H, Mrasek K, Kuechler A, et al: Multitude multicolor chromosome banding (mMCB)-a comprehensive onestep multicolor FISH banding method. Cytogenet Genome Res 103: 34-39 (2003).
24. Weise A, Mrasek K, Fickelscher I, Claussen U, Cheung SW, et al: Molecular definition of high-resolution multicolor banding probes: first within the human DNA sequence anchored FISH banding probe set. J Histochem Cytochem 56: 487-493 (2008).
25. Weksler BB, Subileau EA, Perrière N, Charneau P, Holloway K, et al: Blood-brain barrierspecific properties of a human adult brain endothelial cell line. FASEB J 19: 1872-1874 (2005).
26. Wolburg H, Lippoldt A: Tight junctions of the bloodbrain barrier: development, composition, and regulation. Vascul Pharmacol 38: 323-337 (2002).
27. Zastre JA, Chan GN, Ronaldson PT, Ramaswamy M, Couraud PO, et al: Up-regulation of P-glycoprotein by HIV protease inhibitors in a human brain microvessel endothelial cell line. J Neurosci Res 87: 1023-1036 (2009).