Introducing a true internal standard for the Comet assay to minimize intra-and inter-experiment variability in measures of DNA damage and repair

Nucleic Acids Research

Volumn 37, Issue 22, 2009, Pages

  Zainol, Murizal ^a,b   Stoute, Julia ^a   Almeida, Gabriela M ^a,c   Rapp, Alexander ^d   Bowman, Karen J ^a   Jones, George D D ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b INSTITUTE FOR MEDICAL RESEARCH   (Malaysia)

^c UNIVERSITY OF PORTO   (Portugal)

^d DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; BROXURIDINE; BROXURIDINE ANTIBODY; DNA; UNCLASSIFIED DRUG;

ARTICLE; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DNA REPAIR; FILTER; GENETIC VARIABILITY; GENOTOXICITY; HUMAN; HUMAN CELL; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL; RADIATION DOSE; RADIATION EXPOSURE; RADIATION RESPONSE; X IRRADIATION;

CELL LINE, TUMOR; COMET ASSAY; DATA INTERPRETATION, STATISTICAL; DNA DAMAGE; DNA REPAIR; HUMANS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS;

EID: 75649106826     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkp826     Document Type: Article

References (21)

1. Singh, N.P., McCoy, M.T., Tice, R.R. and Schneider, E.L. (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res., 175, 184-191.
2. Ostling, O. and Johanson, K.J. (1984) Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun., 123, 291-298.
3. Olive, P.L., Banath, J.P. and Durand, R.E. (1990) Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the comet assay. Radiat. Res., 122, 86-94.
4. Collins, A.R., Oscoz, A.A., Brunborg, G., Gaivao, I., Giovannelli, L., Kruszewski, M., Smith, C.C. and Stetina, R. (2008) The comet assay: topical issues. Mutagenesis, 23, 143-151.
5. Tice, R.R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J.C. and Sasaki, Y.F. (2000) Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen., 35, 206-221.
6. Collins, A.R. (2004) The Comet assay for DNA damage and repair-principles, applications, and limitations. Mol. Biotechnol., 26, 249-261.
7. McKenna, D.J., McKeown, S.R. and McKelvey-Martin, V.J. (2008) Potential use of the comet assay in the clinical management of cancer. Mutagenesis, 23, 183-190.
8. Wasson, G.R., McKelvey-Martin, V.J. and Downes, C.S. (2008) The use of the comet assay in the study of human nutrition and cancer. Mutagenesis, 23, 153-162.
9. Kassie, F., Parzefall, W. and Knasmuller, S. (2000) Single cell gel electrophoresis assay: a new technique for human biomonitoring studies. Mutat. Res. Rev. Mutat. Res., 463, 13-31.
10. Azqueta, A., Shaposhnikov, S. and Collins, A.R. (2009) DNA oxidation: investigating its key role in environmental mutagenesis with the comet assay. Mutat. Res. Genet. Toxicol. Environ. Mutagen., 674, 101-108.
11. Collins, A.R. (2009) Investigating Oxidative DNA damage and its repair using the comet assay. Mutat. Res. Rev. Mutat. Res., 681, 24-32.
12. Moneef, M.A., Sherwood, B.T., Bowman, K.J., Kockelbergh, R.C., Symonds, R.P., Steward, W.P., Mellon, J.K. and Jones, G.D. (2003) Measurements using the alkaline comet assay predict bladder cancer cell radiosensitivity. Br. J. Cancer, 89, 2271-2276.
13. Hartley, J.A., Spanswick, V.J., Brooks, N., Clingen, P.H., McHugh, P.J., Hochhauser, D., Pedley, R.B., Kelland, L.R., Alley, M.C., Schultz, R. et al. (2004) SJG-136 (NSC 694501), a novel rationally designed DNA minor groove interstrand cross-linking agent with potent and broad spectrum antitumor activity: part 1: cellular pharmacology, in vitro and initial in vivo antitumor activity. Cancer Res., 64, 6693-6699.
14. Almeida, G.M., Duarte, T.L., Steward, W.P. and Jones, G.D.D. (2006) Detection of oxaliplatin-induced DNA crosslinks in vitro and in cancer patients using the alkaline comet assay. DNA Repair, 5, 219-225.
15. Trzeciak, A.R., Barnes, J. and Evans, M.K. (2008) A modified alkaline comet assay for measuring DNA repair capacity in human populations. Radiat. Res., 169, 110-121.
16. Tiano, L., Littarru, G.P., Principi, F., Orlandi, M., Santoro, L., Carnevali, P. and Gabrielli, O. (2005) Assessment of DNA damage in Down Syndrome patients by means of a new, optimised single cell gel electrophoresis technique. Biofactors, 25, 187-195.
17. De Boeck, M., Touil, N., De Visscher, G., Vande, P.A. and Kirsch-Volders, M. (2000) Validation and implementation of an internal standard in comet assay analysis. Mutat. Res., 469, 181-197.
18. Cebulska-Wasilewska, A., Wiechec, A., Panek, A., Binkova, B., Sram, R.J. and Farmer, P.B. (2005) Influence of environmental exposure to PAHs on the susceptibility of lymphocytes to DNA-damage induction and on their repair capacity. Mutat. Res. Genet. Toxicol. Environ. Mutagen., 588, 73-81.
19. Vaghef, H., Nygren, P., Edling, C., Bergh, J. and Hellman, B. (1997) Alkaline single-cell gel electrophoresis and human biomonitoring for genotoxicity: a pilot study on breast cancer patients undergoing chemotherapy including cyclophosphamide. Mutat. Res. Genet. Toxicol. Environ. Mutagen., 395, 127-138.
20. McKeown, S.R., Robson, T., Price, M.E., Ho, E.T.S., Hirst, D.G. and McKelvey-Martin, V.J. (2003) Potential use of the alkaline comet assay as a predictor of bladder tumour response to radiation. Br. J. Cancer, 89, 2264-2270.
21. Webb, C.F., Jones, G.D.D., Ward, J.F., Moyer, D.J., Aguilera, J.A. and Ling, L.L. (1993) Mechanisms of radiosensitization in bromodeoxyuridine-substituted cells. Int. J. Radiat. Biol., 64, 695-705.