Assessment of DNA damage in Down Syndrome patients by means of a new, optimised single cell gel electrophoresis technique

BioFactors

Volumn 25, Issue 1-4, 2005, Pages 187-195

  Tiano, Luca ^a   Littarru, Gian Paolo ^a   Principi, Federica ^a   Orlandi, Marco ^a   Santoro, Lucia ^a   Carnevali, Paola ^a   Gabrielli, Orazio ^a  

^a UNIVERSITÀ POLITECNICA DELLE MARCHE   (Italy)

Author keywords

Comet assay; DNA damage; Down Syndrome

Indexed keywords

LIQUID NITROGEN;

CHILD; CLINICAL ARTICLE; COMET ASSAY; COMPUTER PROGRAM; CONFERENCE PAPER; CONTROLLED STUDY; CRYOPRESERVATION; DNA DAMAGE; DOWN SYNDROME; HUMAN; HUMAN CELL; IMAGE ANALYSIS; LONGITUDINAL STUDY; LYMPHOCYTE; METHODOLOGY; PRIORITY JOURNAL; QUALITY CONTROL; SCORING SYSTEM; STATISTICAL SIGNIFICANCE;

EID: 33746089903     PISSN: 09516433     EISSN: None     Source Type: Journal    
DOI: 10.1002/biof.5520250122     Document Type: Conference Paper

References (21)

1. S. Brendler-Schwaab, A. Hartmann, S. Pfuhler and G. Speit, The in vivo comet assay: use and status in genotoxicity testing, Mutagenesis 20 (2005), 245-254.
2. A.R. Collins, The comet assay for DNA damage and repair: principles, applications, and limitations, Mol Biotechnol 26 (2004), 249-261.
3. A. Collins, M. Dusinska, M. Franklin et al., Comet assay in human biomonitoring studies: reliability, validation and applications, Env Mol Mutagen 30 (1997), 139-146.
4. J.B. de Haan, B. Susil, M. Pritchard and I. Kola, An altered antioxidant balance occurs in Down syndrome fetal organs: implications for the "gene dosage effect" hypothesis, J Neural Transm Suppl (2003), 67-83.
5. D.W. Fairbairn, P.L. Olive and K.L. O'Neill, The comet assay: a comprehensive review, Mutat Res 339 (1995), 37-59.
6. F. Faust, F. Kassie, S. Knasmuller, S. Kevekordes and V. Mersch-Sundermann, Use of primary blood cells for the assessment of exposure to occupational genotoxicants in human biomonitoring studies, Toxicology 198 (2004), 341-350.
7. F. Faust, F. Kassie, S. Knasmuller, R.H. Boedecker, M. Mann and V. Mersch-Sundermann, The use of the alkaline comet assay with lymphocytes in human biomonitoring studies, Mutat Res 566 (2004), 209-229.
8. O. Garcia, T. Mandina, A.I. Lamadrid, A. Diaz, A. Remigio, Y. Gonzalez, J. Piloto, J.E. Gonzalez and A. Alvarez, Sensitivity and variability of visual scoring in the comet assay. Results of an inter-laboratory scoring exercise with the use of silver staining, Mutat Res 556 (22-11-2004), 25-34.
9. A. Hartmann, E. Agurell, C. Beevers, S. Brendler-Schwaab, B. Burlinson, P. Clay, A. Collins, A. Smith, G. Speit, V. Thybaud and R.R. Tice, 4th International Comet Assay Workshop, Recommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop, Mutagenesis 18 (2003), 45-51.
10. R.C. Iannello, P.J. Crack, J.B. de Haan and I. Kola, Oxidative stress and neural dysfunction in Down syndrome J Neural Transm Suppl 57 (1999), 257-267.
11. S.V. Jovanovic, D. Clements and K. Macleod, Biomarkers of oxidative stress are significantly elevated in down syndrome, Free Rad Biol Med 25 (1998), 1044-1048.
12. P. Moller, Genotoxicity of environmental agents assessed by the alkaline comet assay, Basic Clin Pharmacol Toxicol 96 (Suppl 1) (2005), 1-42.
13. J. O'Brien and A. Zini, Sperm DNA integrity and male infertility, Urology 65 (2005), 16-22.
14. O. Ostling and K.J. Johanson, Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells, Biochem Biophys Res Commun 123 (1984), 291-298.
15. M. Porrini, P. Riso, A. Brusamolino, C. Berti, S. Guarnieri and F. Visioli, Daily intake of a formulated tomato drink affects carotenoid plasma and lymphocyte concentrations and improves cellular antioxidant protection, Br J Nutr 93 (2005), 93-99.
16. R. Seidl, S. Greber, E. Schuller, G. Bernert, N. Cairns and G. Lubec, Evidence against increased oxidative DNA-damage in Down syndrome, Neurosci Lett 235 (1997), 137-140.
17. N.P. Singh, M.T. McCoy, R.R. Tice and E.L. Schneider, A simple technique for quantitation of low levels of DNA damage in individual cells, Exp Cell Res 175 (1988), 184-191.
18. R.R. Tice, E. Agurell, D. Anderson, B. Burlinson, A. Hartmann, H. Kobayashi, Y. Miyamae, E. Rojas, J.C. Ryu and Y.F. Sasaki, Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing, Environ Mol Mutagen 35 (2000), 206-221.
19. X. Zhao, G. Aldini, E.J. Johnson, H. Rasmussen, K. Kraemer, H. Woolf, N. Musaeus, N.I. Krinsky, R.M. Russell and K.J. Yeum, Modification of lymphocyte DNA damage by carotenoid supplementation in postmenopausal women, Am J Clin Nutr 83 (2006), 163-169.
20. M. Montanari, D. Capelli, A. Poloni, D. Massidda et al., Long-term hematologic reconstitution after autologous peripheral blood progenitor cell transplantation: a comparison between controlled-rate freezing and uncontrolled-rate freezing at -80°C, Transfusion 43 (2003), 42-49.
21. H. Tovalin, M. Valverde, M.T. Morandi, S. Blanco, L. Whitehead and E. Rojas, DNA damage in outdoor workers occupationally exposed to environmental air pollutants, Occup Environ Med 63 (2006), 230-236.