A dual-activation, adenoviral-based system for the controlled induction of DNA double-strand breaks by the restriction endonuclease SacI

BioTechniques

Volumn 47, Issue 4, 2009, Pages 847-854

  Maslov, Alexander Y ^a   Metrikin, Maya ^b   Vijg, Jan ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Aging; DNA damage; Double strand breaks; Restriction endonuclease

Indexed keywords

ADENOVIRUS VECTOR; DOUBLE STRANDED DNA; DOXYCYCLINE; GENOMIC DNA; RESTRICTION ENDONUCLEASE; SACI RESTRICTION ENDONUCLEASE; TAMOXIFEN; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; COLORING AGENT; DNA; ENDODEOXYRIBONUCLEASE SACI; SELECTIVE ESTROGEN RECEPTOR MODULATOR; TETRAZOLIUM; THIAZOLE DERIVATIVE; THIAZOLYL BLUE; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; C57BL 6 MOUSE; CELL FATE; CELL SURVIVAL; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DNA RESTRICTION; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC TRANSDUCTION; HUMAN CELL CULTURE; IMMUNOHISTOCHEMISTRY; MAMMAL CELL; MOUSE; NONHUMAN; STREPTOMYCES; STREPTOMYCES ACHROMOGENES; WESTERN BLOTTING; ADENOVIRUS; ANIMAL; ANIMAL EMBRYO; C57BL MOUSE; CELL CULTURE; CULTURE TECHNIQUE; DOSE RESPONSE; DOUBLE STRANDED DNA BREAK; DRUG EFFECT; ENZYME ACTIVATION; FIBROBLAST; FLUORESCENT ANTIBODY TECHNIQUE; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; METABOLISM; TIME; TRANSGENE;

MAMMALIA;

ADENOVIRIDAE; ANIMALS; ANTI-BACTERIAL AGENTS; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CELLS, CULTURED; COLORING AGENTS; COMET ASSAY; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA; DNA BREAKS, DOUBLE-STRANDED; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; DOXYCYCLINE; EMBRYO, MAMMALIAN; ENZYME ACTIVATION; FIBROBLASTS; FLUORESCENT ANTIBODY TECHNIQUE, DIRECT; GENETIC VECTORS; IMMUNOHISTOCHEMISTRY; MICE; MICE, INBRED C57BL; SELECTIVE ESTROGEN RECEPTOR MODULATORS; TAMOXIFEN; TETRAZOLIUM SALTS; THIAZOLES; TIME FACTORS; TRANSFECTION; TRANSGENES;

EID: 75549090551     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113237     Document Type: Article

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