Increase of host cell longevity by the expression of 30K protein originating from silkworm hemolymph in an insect cell-baculovirus system

Enzyme and Microbial Technology

Volumn 35, Issue 6-7, 2004, Pages 581-586

  Kim, Eun Jeong ^a   Park, Tai Hyun ^a  

^a Seoul National University   (South Korea)

Author keywords

30K protein; Apoptosis; Baculovirus; Insect cell; Silkworm; Viability

Indexed keywords

BACULOVIRUS TRANSFER VECTORS; GENE CODING;

DNA; GENES; PROTEINS; VIRUSES;

CELLS;

BETA GALACTOSIDASE; INSECT PROTEIN; PROTEIN 30K; UNCLASSIFIED DRUG; VIRUS DNA;

ANIMAL CELL; APOPTOSIS; BACULOVIRUS EXPRESSION SYSTEM; CELL VIABILITY; CONFERENCE PAPER; CONTROLLED STUDY; FLOW CYTOMETRY; GENE CONSTRUCT; GENETIC CODE; HEMOLYMPH; HOMOLOGOUS RECOMBINATION; HOST CELL; INSECT CELL; LONGEVITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; SILKWORM; VIRUS RECOMBINANT; WILD TYPE;

AUTOGRAPHA CALIFORNICA NUCLEOPOLYHEDROVIRUS; BOMBYX MORI; INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 7544243939     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2004.08.024     Document Type: Conference Paper

References (25)

1. L.K. Miller Baculoviruses: high level expression on insect cells Curr Opin Genet Dev 3b 1993 97 101
2. M.S. Kwon, T. Dojima, and E.Y. Park Comparative characterization og growth and recombinant protein production among three insect cell lines with four kinds of serum free media Biotechnol Bioprocess Eng 8 2003 142 146
3. E.Y. Lee Kinetic analysis of the effect of cell density on hybridoma cell growth in batch culture Biotechnol Bioprocess Eng 7 2002 117 120
4. C.R. Halberstadt, B.O. Palsson, A.R. Midgley, and R.L. Curl Optimization and mathematical modeling of the transtubular bioreactor for the production of monoclonal antibodies from a hybridoma cell line Biotechnol Bioprocess Eng 7 2002 163 170
5. Y.G. Park, T. Tosha, S. Fujita, B. Zhu, H. Iwata, and H.-W. Ryu Effect of low temperature preservation and cell density on metabolic function in a bioartificial liver Biotechnol Bioprocess Eng 8 2003 41 46
6. J.H. Kim, E.J. Kim, and T.H. Park Fed-batch culture of insect cells with exponential feeding of amino acid and yeastolate solution Bioprocess Eng 23 2000 367 370
7. J.H. Kim, and T.H. Park Substrates that limit high density cultures of Spodoptera frugiperda cells Biotechol Tech 13 1999 425 429
8. S.H. Lee, and T.H. Park Growth limiting factors influencing high density culture of insect cells in Grace's medium Biotechnol Lett 16 1994 327 332
9. S.H. Lee, and T.H. Park Two-stage cultivation of insect cells for production of recombinant protein Biotechnol Tech 9 1995 719 724
10. S.H. Ha, T.H. Park, and S.-E. Kim Silkworm hemolymph as a substitute for fetal bovine serum in insect cell culture Biotechnol Tech 10 1996 401 406
11. E.J. Kim, J.-Y. Choi, S.-E. Kim, and T.H. Park Oxidation-deficient silkworm hemolymph as a medium supplement for insect cell culture Biotechnol Bioprocess Eng 3 1998 87 90
12. E.J. Kim, and T.H. Park Reduction of FBS concentration through adaptation process in mammalian cell culture and addition of silkworm hemolymph in insect cell culture J Microbiol Biotechnol 9 1999 227 229
13. S.H. Ha, and T.H. Park Utilization of silkworm hemolymph for production of recombinant protein in an insect cell-baculovirus system Biotechnol Lett 19 1997 1087 1091
14. W.J. Rhee, E.J. Kim, and T.H. Park Kinetic effect of silkworm hemolymph on the delayed host cell death in an insect cell-baculovirus system Biotechnol Prog 15 1999 1028 1032
15. W.J. Rhee, and T.H. Park Silkworm hemolymph inhibits baculovirus-induced insect cell apoptosis Biochem Biophys Res Commun 271 2000 186 190
16. E.J. Kim, W.J. Rhee, and T.H. Park Isolation and characterization of an apoptosis-inhibiting component from the hemolymph of Bombyx mori Biochem. Biophys. Res Commun 285 2001 224 228
17. S. Izumi, J. Fujie, S. Yamada, and S. Tomino Molecular properties and biosynthesis of major plasma proteins in Bombyx mori Biochim Biophys Acta 670 1981 222 229
18. E.J. Kim, H.J. Park, and T.H. Park Inhibition of apoptosis by recombinant 30K protein originating from silkworm hemolymph Biochem Biophys Res Commun 308 2003 523 528
19. H.J. Park, E.J. Kim, T.Y. Koo, and T.H. Park Purification of anti-apoptotic recombinant 30K protein produced in Escherichia coli and its anti-apoptotic effect in mammalian and insect cell systems Enzyme Microb Technol 33 2003 466 471
20. E.J. Kim, W.J. Rhee, and T.H. Park Inhibition of apoptosis by a Bombyx mori gene Biotechnol Prog 20 2004 324 329
21. E.J. Kim, and T.H. Park Anti-apoptosis engineering Biotechnol Bioprocess Eng 8 2003 76 82
22. U.K. Laemmli Cleavage of structural proteins during the as assembly of the head of bacteriophage T4 Nature 227 1970 680 685
23. M.S. Peterson, M.-D. Kim, K.C. Han, J.H. Kim, and J.H. Seo Flow cytometric analysis of human lysozyme production in recombinant Saccharomyces cerevisiae Biotechnol Bioprocess Eng 7 2002 52 55
24. W.J. Rhee, E.J. Kim, and T.H. Park Silkworm hemolymph as a potent inhibitor of apoptosis in Sf9 cells Biochem Biophys Res Commun 295 2002 779 783
25. S.S. Choi, W.J. Rhee, and T.H. Park Inhibition of human cell apoptosis by silkworm hemolymph Biotechnol Prog 18 2002 874 878