Purification of recombinant 30K protein produced in Escherichia coli and its anti-apoptotic effect in mammalian and insect cell systems

Enzyme and Microbial Technology

Volumn 33, Issue 4, 2003, Pages 466-471

  Park, Hye Jung ^a   Kim, Eun Jeong ^a   Koo, Tai Young ^a   Park, Tai Hyun ^a  

^a Seoul National University   (South Korea)

Author keywords

30K protein; Apoptosis; Inclusion body; Purification; Silkworm hemolymph

Indexed keywords

AFFINITY CHROMATOGRAPHY; ANIMAL CELL CULTURE; CLONING; COLUMN CHROMATOGRAPHY; ESCHERICHIA COLI;

GENE CODINGS;

PROTEINS;

HISTIDINE; PROTEIN 30K; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

APOPTOSIS; BACTERIAL GENE; CARBOXY TERMINAL SEQUENCE; CELL INCLUSION; CELL PROTECTION; CONFERENCE PAPER; CULTURE MEDIUM; DRUG EFFECT; ESCHERICHIA COLI; GENE FUSION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; INSECT CELL; MAMMAL CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DENATURATION; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN PURIFICATION; QUANTUM YIELD; SEPARATION TECHNIQUE;

BOMBYX; BOMBYX MORI; ESCHERICHIA COLI; INSECTA; MAMMALIA;

EID: 0042932424     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(03)00149-2     Document Type: Conference Paper

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