Substrates that limit high density cultures of Spodoptera frugiperda cells

Biotechnology Techniques

Volumn 13, Issue 6, 1999, Pages 425-429

  Kim, Jai Hyun ^a   Park, Tai Hyun ^b  

^a Sungkyunkwan University   (South Korea)

^b Seoul National University   (South Korea)

Author keywords

High density culture; Limiting substrates; Spodoptera frugiperda

Indexed keywords

AMINO ACID ANALYSIS; CULTIVATION; CULTURE MEDIUM; FETAL BOVINE SERUM; HIGH DENSITY CULTURE; SECONDARY METABOLITE;

EID: 0032789732     PISSN: 0951208X     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008964318308     Document Type: Article

References (13)

1. Bedard C, Tom R, Kamen A (1993) Growth, nutrient consumption, and end-product accumulation in Sf-9 and BTI-EAA insect cell cultures: insights into growth limitation and metabolism. Biotechnol. Prog. 9: 625-624.
2. Clements AN, Grace TDC (1967) The utilization of sugars by insect cells in culture. J. Insect Physiol. 13: 1327-1332.
3. Drews M, Paalme T, Vilu R (1995) The growth and nutrient utilization of the insect cell line Spodoptera frugiperda Sf9 in batch and continuous culture. J. Biotechnol. 40: 187-198.
4. Ha SH, Park TH (1997) Efficient production of recombinant protein in Spodoptera frugiperda/AcNPV system utilizing silkworm hemolymph. Biotechnol. Lett. 19: 1087-1091.
5. Ha SH, Park TH, Kim S-E (1996) Silkworm hemolymph as a substitute for fetal bovine serum in insect cell cuture. Biotechnol. Tech. 10: 401-106.
6. Kim EJ, Park TH (1999) Reduction of FBS concentration through adaptation process in mammalian cell culture and addition of silkworm hemolymph in insect cell culture. J. Microbiol. Biotechnol. 9: 227-229.
7. King GA, Daugulis AJ, Faulkner P, Goosen MFA (1988) Growth of baculovirus-infected insect cells in microcapsules to a high cell and virus density. Biotechnol. Lett. 10: 683-688.
8. Kloppinger J, Fertig G, Fraune E, Miltenburger HG (1990) High cell density perfusion culture of insect cells for production of baculovirus and recombinant proteins. In: Spier AE, Griffiths JB, Meignier B, eds. Production of Biologicals from Animal Cells in Culture. Oxford: Butterworth-Heinemann Press, pp. 470-475.
9. Lee SH, Park TH (1994) Growth limiting factors influencing high density culture of insect cells in Grace's medium. Biotechnol. Lett. 16: 327-332.
10. Lee SH, Park TH (1995) Two-phase cultivation of insect cells for production of recombinant protein. Biotechnol. Techn. 9: 719-724.
11. Maiorella B, Inlow D, Shauger A, Harano D (1988) Large-scale insect cell-culture for recombinant protein production. Bio/technol. 6: 1406-1410.
12. Murhammer DW, Goochee CF (1988) Scaleup of insect cell cultures: protective effects of Pluronic F-68. Bio/technol. 6: 1411-1418.
13. Summers MD, Smith GE (1987) A Manual Method for Baculovirus Vectors and Insect Cell Culture Procedures. College Station: Texas A&M University.