Reduction of FBS concentration through adaptation process in mammalian cell culture and addition of silkworm hemolymph in insect cell culture

Journal of Microbiology and Biotechnology

Volumn 9, Issue 2, 1999, Pages 227-229

  Kim, Eun Jeong ^a   Park, Tai Hyun ^a  

^a Seoul National University   (South Korea)

Author keywords

Adaptation; Animal cell; Fetal bovine serum; Silkworm hemolymph

Indexed keywords

ADAPTATION; ANIMAL CELL; ARTICLE; CELL CULTURE; CELL GROWTH; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; FETAL CALF SERUM; HEMOLYMPH; INSECT; MAMMAL CELL; NONHUMAN; SILKWORM;

EID: 0041859270     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (7)

1. Ha, S. H. and T. H. Park. 1997. Efficient production of recombinant protein in Spodoptera frugiperda/AcNPV system utilizing silkworm hemolymph. Biotechnol. Lett. 19: 1087-1091.
2. Ha, S. H., T. H. Park, and S.-E. Kim. 1996. Silkworm hemolymph as a substitute for fetal bovine serum in insect cell culture. Biotechnol. Tech. 10: 401-406.
3. Hu, W.-S., W. Zhou, and L. F. Europa. 1998. Controlling mammalian cell metabolism in bioreactors. J. Microbiol. Biotechnol. 8: 8-13.
4. Lee, S. H. and T. H. Park. 1994. Two-phase cultivation of insect cells for production of recombinant protein. Biotechnol. Tech. 10: 719-724.
5. Lee, S. H. and T. H. Park. 1995. Growth limiting factors influencing high density culture of insect cells in Graces medium. Biotechnol. Lett. 4: 327-332.
6. Murhammer, D. W. and C. F. Goochee. 1988. Scale-up of insect cell cultures: Prospective effects of Pluronal F-68. Bio/technol. 6: 1411-1418.
7. Zhang, J., N. Kalogerakis, L. Behie, and K. Latrou. 1992. Investigation of reduced serum and serum-free media for the cultivation of insect cells (Bm 5) and the production of baculovirus (BmNPV). Biotechnol. Bioeng. 40: 1165-1172.