Recombinant adeno-associated virus serotype 2 effectively transduces primary rat brain astrocytes and microglia

Brain Research Protocols

Volumn 14, Issue 1, 2004, Pages 18-24

  Gong, Yan ^a   Chen, Shuzhen ^b   Sonntag, Christopher F ^b   Sumners, Colin ^b   Klein, Ronald L ^c   King, Michael A ^b,d   Hughes, Jeffrey A ^a   Meyer, Edwin M ^b  

^a UNIVERSITY OF FLORIDA   (United States)

^b UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

^c LOUISIANA STATE UNIVERSITY HEALTH SCIENCES CENTER   (United States)

^d VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

Astrocytes; Green fluorescent protein; Microglia; Primary neurons; Recombinant adeno associated virus 2

Indexed keywords

BETA ACTIN; GREEN FLUORESCENT PROTEIN;

ADENO ASSOCIATED VIRUS; ANIMAL CELL; ARTICLE; ASTROCYTE; BRAIN CELL; BRAIN NERVE CELL; CELL CULTURE; CELL INTERACTION; CELL TYPE; CHICKEN; COCULTURE; CONTROLLED STUDY; GENETIC MANIPULATION; MICROGLIA; NONHUMAN; PRIORITY JOURNAL; RAT; SEROTYPE; VIRUS RECOMBINANT;

ANIMALS; ASTROCYTES; CELLS, CULTURED; DEPENDOVIRUS; GREEN FLUORESCENT PROTEINS; HUMANS; MICROGLIA; NEURONS; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT PROTEINS; TRANSDUCTION, GENETIC;

EID: 7444235514     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.brainresprot.2004.08.001     Document Type: Article

References (17)

1. J.S. Bartlett, R.J. Samulski, and T.J. McCown Selective and rapid uptake of adeno-associated virus type 2 in brain Hum. Gene Ther. 9 1998 1181 1186
2. S. Chen, D. Luo, W.J. Streit, and J.K. Harrison TGF-beta1 upregulates CX3CR1 expression and inhibits fractalkine-stimulated signaling in rat microglia J. Neuroimmunol. 133 2002 46 55
3. Y.C. Chou, W.C. Luttge, and C. Sumners Characterization of glucocorticoid type II receptors in neuronal and glial cultures from rat brain J. Neuroendocrinol. 2 1990 29 38
4. A. Chtarto, H.U. Bender, C.O. Hanemann, T. Kemp, E. Lehtonen, M. Levivier, J. Brotchi, T. Velu, and L. Tenenbaum Tetracycline-inducible transgene expression mediated by a single AAV vector Gene Ther. 10 2003 84 94
5. M. Cucchiarini, X.L. Ren, G. Perides, and E.F. Tervilliger Selective gene expression in brain microglia mediated via adeno-associated virus type 2 and type 5 vectors Gene Ther. 10 2003 657 667
6. B.L. Davidson, C.S. Stein, J.A. Heth, I. Martins, R.M. Kotin, T.A. Derksen, J. Zabner, A. Ghodsi, and J.A. Chiorini Recombinant adeno-associated virus type 2, 4, and 5 vectors: transduction of variant cell types and regions in the mammalian central nervous system Proc. Natl. Acad. Sci. U. S. A. 97 2000 3428 3432
7. D. Grimm, A. Kern, K. Rittner, and J.A. Kleinschmidt Novel tools for production and purification of recombinant adenoassociated virus vectors Hum. Gene Ther. 9 1998 2745 2760
8. R.L. Klein, M.E. Hamby, Y. Gong, A.C. Hirko, S. Wang, J.A. Hughes, M.A. King, and E.M. Meyer Dose and promoter effects of adeno-associated viral vector for green fluorescent protein expression in the rat brain Exp. Neurol. 176 2002 66 74
9. D.M. Kube, and A. Srivastava Quantitative DNA slot blot analysis: inhibition of DNA binding to membranes by magnesium ions Nucleic Acids Res. 25 1997 3375 3376
10. S. Kugler, P. Lingor, U. Scholl, S. Zolotukhin, and M. Baehr Differential transgene expression in brain cells in vivo and in vitro from AAV-2 vectors with small transcriptional control units Virology 311 2003 89 95
11. H. Lassmann, M. Schmied, K. Vass, and W.F. Hickey Bone marrow derived elements and resident microglia in brain inflammation Glia 7 1993 19 24
12. K. Qing, C. Mah, J. Hansen, S. Zhou, V. Dwarki, and A. Srivastava Human fibroblast growth factor receptor 1 is a co-receptor for infection by adeno-associated virus 2 Nat. Med. 5 1999 71 77
13. P.D. Robbins, and S.C. Ghivizzani Viral vectors for gene therapy Pharmacol. Ther. 80 1998 35 47
14. M.J. Ruitenberg, R. Eggers, G.J. Boer, and J. Verhaagen Adeno-associated viral vectors as agents for gene delivery: application in disorders and trauma of the central nervous system Methods 28 2002 182 194
15. C. Summerford, and R.J. Samulski Membrane-associated heparan sulfate proteoglycan is a receptor for adeno-associated virus type 2 virions J. Virol. 72 1998 1438 1445
16. C. Summerford, J.S. Bartlett, and R.J. Samulski AlphaVbeta5 integrin: a co-receptor for adeno-associated virus type 2 infections Nat. Med. 5 1999 78 82
17. S. Zolotukhin, B.J. Byrne, E. Mason, I. Zolotukhin, M. Potter, K. Chesnut, C. Summerford, R.J. Samulski, and N. Muzyczka Recombinant adeno-associated virus purification using novel methods improves infectious titer and yield Gene Ther. 6 1999 973 985