Activated caspases are present in frozenthawed canine sperm and may be related to post thaw sperm quality

Zygote

Volumn 17, Issue 4, 2009, Pages 297-305

  Sokolowska, A ^b   García, B MacÍas ^b   Fernández, L González ^b   Ortega Ferrusola, C ^b   Tapia, J A ^b   Pena F J ^a,b  

^a Avd de la Universidad s/n   (Spain)

^b UNIVERSITY OF EXTREMADURA   (Spain)

Author keywords

Apoptosis; Canine; Caspases; Flow cytometry; Freezing; Mitochondria; Sperm

Indexed keywords

CANIS FAMILIARIS;

BENZOXAZOLE DERIVATIVE; CASPASE; DRUG DERIVATIVE; ETHIDIUM; ETHIDIUM HOMODIMER; QUINOLINE DERIVATIVE; YO PRO 1; YO-PRO 1;

ANIMAL; ANIMAL DISEASE; APOPTOSIS; ARTICLE; CRYOPRESERVATION; CYTOLOGY; DOG; FLOW CYTOMETRY; MALE; METABOLISM; MITOCHONDRION; SPERM PRESERVATION; SPERMATOZOON; SPERMATOZOON MOTILITY;

ANIMALS; APOPTOSIS; BENZOXAZOLES; CASPASES; CRYOPRESERVATION; DOGS; ETHIDIUM; FLOW CYTOMETRY; MALE; MITOCHONDRIA; QUINOLINIUM COMPOUNDS; SEMEN PRESERVATION; SPERM MOTILITY; SPERMATOZOA;

EID: 74149089855     PISSN: 09671994     EISSN: 14698730     Source Type: Journal    
DOI: 10.1017/S0967199409005401     Document Type: Article

References (33)

1. Cisternas, P. & Moreno, R.D. (2006). Comparative analysis of apoptotic pathways in rat mouse and hamster spermatozoa. Mol. Reprod. Develop. 73, 1318-1325
2. Cyali, S., Sakkas, D., Vigue, L., Demir, L. & Huszar, G. (2004). Cellular maturity and apoptosis in human sperm: creatine kinase, caspase-3 and BCL-XL levels inmature and diminished maturity sperm. Mol. Hum. Reprod. 10, 365-372
3. Desagher, S. & Martinou, J.C. (2000). Mitochondria as the central control point of apoptosis. Trends Cell Biol. 10, 369-377
4. Grunewald, S., Paasch, U., Said, T.M., Sharma, RK., Glandler, H.J. & Agarwal, A. (2005). Caspase activation in human spermatozoa in response to physiological and pathological stimuli. Fertil. Steril. Suppl. 1, 1106-1112
5. Holt, W.V. & Van Look, K.J.W. (2004). Concepts in sperm heterogeneity, sperm selection and sperm competition as biological foundations for laboratory tests of semen quality. Reproduction 127, 527-535.
6. Hu, X.Y., Xu, Y.M., Qiao, Y.,Wu, D.L., Sa, Y.L., Fu, Q., Yu, J.J., Zhang, X.R., Zhang, J., Gu, B.J., Chen, R. & Xie, H. (2006). Reduced semen quality in chronic prostatitis patients that induce the release of apoptotic protein Omi/HtrA2 from spermatozoa. Prostate cancer and prostatic diseases. doi:10.1038/sj.pcan.4500919.
7. Idziorek, T., Estaquier, J., de Bels, F. & Ameisen, JC. (1995). YOPRO-1 permits cytofluorometric analysis of programmed cell dead (apoptosis) without interfering with cell viability. J. Immunol. Meth. 185, 249-285
8. Konrad, L., Keilani, M.M., Laible, L., Nottelmann, U. & Hofmann, R. (2006). Effects of TGF betas and a specific antagonist on apoptosis of immature ratmale germ cells in vitro. Apoptosis 11, 739-748
9. Marchetti, C., Gallego, M.A., Defossez, A., Formstecher, P. & Marchetti, P. (2004). Staining of human sperm with fluorochrome labelled inhibitor of caspases: correlation with apoptosis and sperm parameters. Hum. Reprod. 19, 1127-1134
10. Marti, E., Perez Pe, R., Muino Blanco, T. & Cebrian Perez, JA. (2006). Comparative study of four different sperm washing methods using apoptotic makers in ram spermatozoa. J. Androl. 27, 746-753
11. Martin, G., Cagnon, N., Sabido, O., Sion, B., Grizard, G., Durand, P.&Levy, R. (2007).Kinetics of occurrence of some features of apoptosis during the cryopreservation process of bovine spermatozoa. Hum. Reprod. 22, 380-388
12. Martin, G., Sabido, O, Durand, Ph. & Levy, R. (2004). Cryopreservation induces an apoptosis like mechanism in bull sperm. Biol. Reprod. 71, 28-37.
13. Martin, G., Sabido, O., Durand, P. & Levy, R. (2005). Phosphatidylserine externalization in human semen induced by calcium ionophore A23187: relationship with apoptosis, membrane scrambling and the acrosome reaction. Biol. Reprod. 20, 3459-3468
14. Mazur, P. (1984). Freezing of living cells: mechanism and implications. Am. J. Physiol. 16, C125-42.
15. Ńünez Martinez, I., Moŕan, J.M. & Pena, F.J. (2007). Sperm indexes obtained using computer assisted morphometry provide a forecast of the freezeability of canine sperm. Int. J. Androl. 30, 182-189
16. Ńünez Martinez, I., Moran, J.M. & Pẽna, F.J. (2005). Do computer assisted derived morphometric sperm characteristics reflect DNA status in canine spermatozoa? Reprod. Dom. Anim. 40, 37-43.
17. Ńünez Martinez, I., Moran, J.M. & Pẽna, F.J. (2006). A three step statistical procedure to identify sperm kinematic subpopulations in canine ejaculates: changes after cryopreservation. Reprod. Dom. Anim. 41, 408-415
18. Ńünez-Mart́inez, I., Moŕan, J.M. & Pẽ na, F.J. (2006). Two step cluster procedure after principal component analysis identifies sperm subpopulations in canine ejaculates and its relation to cryoresistance. J. Androl. 27, 596-603.
19. Ormerod, M.G., Sun, X.M., Snowden, R.T., Davies, R., Fearnhead, H. & Cohen, G.M. (1993). Increased membrane permeability of apoptotic thymocites: a flow cytometric study. Cytometry 14, 595-602.
20. Ortega-Ferrusola, C., Sotillo-Gaĺan, Y., Varela-Ferńandez, E., Gallardo-Bolã nos, J.M.,Gonźalez-Ferńandez, L., Tapia, J.A. & Pẽ na, F.J. (2008). Detection of apoptosis like changes during the cryopreservation process in equine sperm. J. Androl. 29, 213-221
21. Paasch, U., Sharma, R.K., Gupta, A.K., Grunewald, S., Mascha, E.J., Thomas, A.J., Glander, H.J. & Agarwal, A. (2004). Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa. Biol. Reprod. 71, 1828-1837
22. Pẽ na, A. & Linde-Forsberg, C. (2000). Effect of spermatozoal concentration and post-thaw dilution rate on survival after thawing of dog spermatozoa. Theriogenology 54, 703-718 (Pubitemid 30848278)
23. Pẽ na, A.I. (2004). Canine fresh and cryopreserved evaluation. Anim. Reprod. Sci. 82-3, 209-224
24. Pẽ na, F.J., Johanisson, A., Wallgren, M. & Rodriguez Martinez, H. (2003). Antioxidant supplementation in vitro improves boar sperm motility, and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate. Anim. Reprod. Sci. 78, 85-88
25. Pẽ na, F.J., Johannisson, A., Wallgren, M. & Rodriguez Martinez,H. (2003).Assessment of fresh and frozen thawed boar semen using an annexin assay: a new method of evaluating sperm membrane integrity. Theriogenology 60, 677-689
26. Pẽ na, F.J., Johannisson, A., Wallgren, M. & Rodriguez Martinez, H. (2004). Antioxidant supplementation of boar spermatozoa from different fractions of the ejaculate improves cryopreservation: changes in sperm membrane lipid architecture. Zygote 12, 117-127
27. Pẽ na, F.J., Johannisson, A., Wallgren, M. & Rodriguez Martinez, H. (2005). A new and simple method to evaluate early membrane changes in frozen-thawed boar spermatozoa. Int. J. Androl. 28, 107-114
28. Pena, F.J., Ńünez Martinez, I. & Moŕan, J.M. (2006). Semen technologies in dog breeding: an update. Reprod. Dom. Anim. 41(S2), 21-29
29. Said, T.M., Paasch, U., Glander, H.J. & Agarwal, A. (2004). Role of caspases in male infertility. Hum. Reprod. Update 10, 39-51.
30. Watson, P.F. (2000). The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci. 60-1, 481-492
31. Wronski, R.,Golob, N.,Grygar, E.&Winsdish, M. (2002). Two color, fluorescence-based microplate assay for apoptosis detection. Biotechniques 32, 666-668
32. Wundrich, K., Paasch, U., Leicht, M. & Glander, H.J. (2006). Activation of caspases in human spermatozoa during cryopreservation: an inmunoblot study. Cell Tissue Bank 7, 81-90.
33. Zheng, S., Turner, T.T. & Lysiak, J.J. (2006). Caspase 2 activity contributes to the initialwave of germ cell apoptosis during the first round of spermatogenesis. Biol. Reprod. 74, 1026-1033